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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (3): 237-243.doi: 10.11924/j.issn.1000-6850.2013-0603

• 生物技术科学 • 上一篇    下一篇

PVA降解菌株的筛选鉴定及其固定化工艺研究

惠小双 李术娜 李红亚 朱宝成   

  • 收稿日期:2013-03-06 修回日期:2013-04-23 出版日期:2014-01-25 发布日期:2014-01-25
  • 基金资助:
    河北农业大学青年科学基金(QJ201233)。

Isolation, Identification and Immobilized of Efficient PVA-degrading Bacteria

  • Received:2013-03-06 Revised:2013-04-23 Online:2014-01-25 Published:2014-01-25

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摘要： 为了得到一株降解PVA的芽孢杆菌，并对其进行固定化研究。利用PVA碘-硼酸平板法初筛，降解率测定法进行复筛，并通过正交试验探索其固定化工艺。经初筛得到8株可降解PVA的细菌，复筛出1株具有较高降解率的菌株3-9，48 h降解率为86.56%。该菌株鉴定为枯草芽孢杆菌(Bacillus subtilis subsp.subtilis)。该菌株的最佳固定化条件为：4%海藻酸钠，3%CaC12，包埋0.1 g的湿菌体，钙化时间4 h。结果表明固定化后的PVA降解菌比游离菌的降解性能提高了14.02%。本研究得到了一株高效降解聚乙烯醇的枯草芽孢杆菌，并确定其最佳的固定化条件，为PVA污水处理提供了菌种资源和理论基础。

关键词: 化感效应, 化感效应

Abstract: In order to get a strain of degrading PVA bacillus, and immobilization of the strain resources. Using PVA iodine-boric acid plate method for screening, measures degradation for rescreening, and explored the immobilization process by orthogonal experiment. Eight bacterial strains showed degrade activity to polyvinyl alcohol, and 3-9 was obtained during the rescreening. After 48 hours, PVA degradation rate up to 86.56%. This strain was identified as Bacillus subtilis subsp.. The optimum immobilization conditions for this strain are as follows: sodium alginate of 4%, CaC12 of 3%, cells of 0.1 g and calcification time was 4 hours. The results showed that degradation rate increased by 14.02% after immobilized.This research was supported by an efficient degradation of polyvinyl alcohol Bacillus subtilis subsp., and determined the optimal immobilization conditions，provided resources and theoretical basis for PVA wastewater treatment.