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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (34): 50-55.doi: 10.11924/j.issn.1000-6850.2013-0867

所属专题： 烟草种植与生产； 农业生态

• 林学 园艺 园林 • 上一篇    下一篇

烤烟品种‘Oxford207’青枯病抗性的遗传分析与分子标记初选

范江 刘勇 童治军 李永平   

  • 收稿日期:2013-03-27 修回日期:2013-05-27 出版日期:2013-12-05 发布日期:2013-12-05
  • 基金资助:
    中国烟草总公司云南省公司科技项目;云南省社会发展科技计划

Inheritance of Resistance to Ralstonia solanacearum in Flue-cured tobacco Oxford207 and Molecular Marker Screen

  • Received:2013-03-27 Revised:2013-05-27 Online:2013-12-05 Published:2013-12-05

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摘要： 为了研究烟草青枯病抗性的遗传特性和开发抗性相关分子标记，以抗青枯病的烟草品种‘Oxford207’，感病品种‘红花大金元’为亲本，构建了F1、F2和BC1群体，并采用苗期恒温水培接种法鉴定了群体青枯病的抗性。结果表明，接种后定期调查的F1、F2和BC1F1的死亡率比较接近，均稳定介于抗病亲本和感病亲本之间。F1、F2和BC1F1死亡率曲线下面积比较接近，同样介于抗病与感病亲本之间。表明‘Oxford207’的青枯病抗性不符合典型的显性或隐性基因控制模型，属于加性基因控制。采用简单重复序列(SSR)和分离群体分组分析法初步筛选了抗性相关的分子标记。从800多条SSR引物中，筛选出263个在抗感亲本间有多态性且在F1中呈共显性的引物、3个在抗感池之间有多态性的引物。

关键词: 抽样技术, 抽样技术

Abstract: Tobacco variety Oxford207 and Honghuadajingyuan(HD), which resistant to bacterial wilt(BW) and susceptible to BW respectively were used as parents. F1, F2, BC1 populations were constructed. The resistance of parents and populations were evaluated by temperature-controlled water inoculation Ralstonia solanacearum at seedling stage. The death rates of F1, F2 and BC1F1 are similar, which are steadily between resistant and susceptible parents’ regular surveys. The area under the disease curve of F1, F2 and BC1F1 are similar, which are steadily between resistant and susceptible parents’ also. Those data indicate that the resistance of Oxford207 to bacterial wilt is additive, not typical dominant and recessive. Simple sequence repeat (SSR) technology and bulked segregant analysis were applied to identify markers linked to the resistance gene. Among more than 800 pieces of SSR primers, 263 primers have polymorphism between resistant and susceptible parents and show co-dominant in F1, 3 primers have polymorphism between the resistant pool and susceptible pool of F2 individual plants.