温敏核不育水稻eui突变体自交结实期籽粒形成生理研究

doi:10.11924/j.issn.1000-6850.2013-1758

摘要/Abstract

摘要： 为了探讨温敏核不育水稻eui突变繁殖时籽粒形成生理，以‘培矮64S’为对照，采用冷水灌溉繁殖的方法研究了温敏核不育水稻(Oryza sativa)eui突变体（‘长选3S’）自交结实期籽粒形成的生理特点。结果表明，在灌浆结实过程中，‘长选3S’和‘培矮64S’籽粒直链淀粉、支链淀粉含量在花后第7~27天增加快，随后增加减慢；籽粒中ADPG焦磷酸化酶、谷氨酰胺合成酶、谷氨酸合酶等3个酶的酶活性及可溶性蛋白质含量随灌浆进程逐渐下降；籽粒中可溶性淀粉合成酶、淀粉分支酶的酶活性随灌浆进程先升后降，呈单峰曲线变化。就整个灌浆结实期而言，‘长选3S’籽粒中ADPG焦磷酸化酶、可溶性淀粉合成酶、淀粉分支酶、谷氨酰胺合成酶、谷氨酸合酶等5个酶的酶活性及直、支链淀粉含量、可溶性蛋白质含量均比其亲本‘培矮64S’的高，而自交结实率、单株产量则比‘培矮64S’的低。差异性分析表明，2个不育系之间籽粒中上述5个酶的酶活性及直、支链淀粉含量、自交结实率及单株产量差异不显著，而可溶性蛋白质含量差异显著。

关键词: RAPD, RAPD

Abstract: In order to investigate the grain formation physiology of thermo-sensitive genic male sterile (TGMS) rice with the elongated uppermost internode (eui) gene during grain filling period. TGMS rice ‘Changxuan 3S’, possessing eui gene, was studied by using low temperature underground water to irrigate TGMS rice line ‘Changxuan 3S’ to multiply it, with its parent ‘Pei’ai 64S’ used as a control. The results showed that, With the process of grain filling, the contents of amylase and amylopectin increased quickly at 7-27 days after flowering, then increased slowly and stopped increasing at 31 days after flowering, and the soluble protein content and the activities of the ADPG pyrophosphorylase (ADPG-PPase), glutamine synthetase (GS) and glutamate synthase (GOGAT) decreased gradually, and the activities of the soluble starch synthase (SSS) and starch branching enzyme (SBE) increased gradually and then declined slowly, showing a single-peak curve in the grains of ‘Changxuan 3S’ and ‘Pei’ai 64S’. Throughout the filling stage, the contents of amylase, amylopectin and soluble protein and the activities of ADPG-PPase, SSS, SBE, GS, GOGAT in the grains of ‘Changxuan 3S’ were higher than those of ‘Pei’ai 64S’, but its bagged seed set and per plant yield were lower than those of ‘Pei’ai 64S’. Differences analysis showed that the differences of the 5 enzymes activities, amylase content, amylopectin content, bagged seed set and per plant yield were not obvious, and the difference of soluble protein content was obvious in filling grains of ‘Changxuan 3S’ and ‘Pei’ai 64S’.

肖辉海郝小花王云王文龙程新奇赵东海. 温敏核不育水稻eui突变体自交结实期籽粒形成生理研究[J]. 中国农学通报, 2014, 30(9): 48-54.

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