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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (8): 17-22.doi: 10.11924/j.issn.1000-6850.2013-2005

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

大肠杆菌生物膜毒力基因的检测

喻华英 张亮亮   

  • 收稿日期:2013-07-23 修回日期:2013-09-22 出版日期:2014-03-15 发布日期:2014-03-15
  • 基金资助:
    国家自然基金 “新疆地区犊牛腹泻病中大肠杆菌基因型分型及 bfpA基因生物学功能探讨” (31060344)。

Checking Virulence Genes of Biofilm in Escherichia coli

  • Received:2013-07-23 Revised:2013-09-22 Online:2014-03-15 Published:2014-03-15

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摘要： 通过确定大肠杆菌形成生物膜毒力基因的分布情况，为大肠杆菌生物膜的形成机理提供理论依据。本实验利用PCR聚合酶链式反应，对25株大肠杆菌阳性菌株菌进行了相关毒力因子fimH，Kp sMTII，chuA，fliC，yja和PapC的检测筛选。其结果显示：KpsMTII在所检测25株菌中出现的频率最高，占40%；其次是fliC，占32.5%，fimH占20%，合计占52.5%；chuA占7.5%，yja和PapC均未检出。结果表明：细菌粘附结构fimH基因和fliC基因是影响大肠杆菌形成生物膜的主要粘附结构毒力基因，其次是大肠杆菌毒力因子KpsMTII基因，最后是外膜蛋白血红素chuA基因。

关键词: 分生孢子, 分生孢子

Abstract: The test was aimed of confirmed these genes distribution condition in positive biofilm with 25 strains of Escherichia coli, and then provided the theoretical basis of the mechanism of biofilm during the pathopoiesia in the Escherichia coli. The polymerase chain reaction was used of being checked virulence genes in the fimH, KpsMTII, chuA, fliC, yja and PapC in 25 strains of Escherichia coli which were positive biofilm. The results showed that, Firstly, the gene of KpsMTII aroused frequence was higjhest which account of 40%; Secondly that the gene of fliC account of 32.5% and the gene of fimH account of 20%, the two genes total was 52.5%; the gene of chuA account of 7.5 %, the genes of yja and PapC unchecked in this test. In this test the biofilm formed were firstly effected in adhere structure genes such as fimH and fliC. Secondly effected gene was KpsMTII, Finally, effected gene was outer membrane protein gene chuA in 25 strains Escherichia coli.