欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (31): 316-320.doi: 10.11924/j.issn.1000-6850.2014-1946

所属专题： 生物技术

• 植物保护 农药 • 上一篇    

魔芋灰霉病致病菌及其生理特性的研究

降巧龙,杨朝柱,赵青华,刘二喜,覃宇,刘小芳,潘亚妮   

  1. 湖北省恩施州农业科学院,恩施土家族苗族自治州农业科学院,恩施州农业科学院,恩施土家族苗族自治州农业科学院,湖北省恩施州农业科学院,湖北省恩施州农业科学院,湖北恩施自治州农业科学院
  • 收稿日期:2014-07-15 修回日期:2014-07-15 接受日期:2014-09-09 出版日期:2014-11-20 发布日期:2014-11-20
  • 通讯作者: 降巧龙
  • 基金资助:
    恩施州农科院青年科技创新基金“魔芋灰霉病致病菌分子鉴定与防控药物筛选”(2013-118-4-15)；湖北省魔芋产业发展专项“魔芋种芋繁育基地建设及高效栽培技术集成示范”(014-010)；2013 湖北省农业科技创新中心项目“魔芋新品种选育与示范”(014-620-001-003)

Biology Characteristics of Botrytis cinerea Pathogens on Amorphophallus Konjac

  • Received:2014-07-15 Revised:2014-07-15 Accepted:2014-09-09 Online:2014-11-20 Published:2014-11-20

摘要： 为探明魔芋灰霉病的发病规律，对其发病症状、致病菌及其生理特性进行了较系统的研究。结果表明，魔芋灰霉病菌为葡萄孢菌属(Botryotinia)；其分生孢子萌发的温度范围为10~30℃，最适温度为20~25℃；在pH 4~9条件下均能萌发，最适pH为5；在各种营养液中均能萌发，在2%的蔗糖液中萌发最好，其次为2%的葡萄糖和魔芋甘露低聚糖；分生孢子的致死温度为57℃、5 min。魔芋灰霉病菌菌丝在4种培养基上均能生长，其中PDA培养基最适宜菌丝生长，最适的产孢培养基为PDA+魔芋甘露低聚糖；菌丝在5~30℃均能生长和产孢，适宜温度为20~25℃；在pH 4~9均能生长及产孢，最适pH为5。

关键词: 气候变暖, 气候变暖, 气温, 积温, 降水, 日照, 甘肃中部

Abstract: In order to clarify the pathogenesis of konjac Botrytis cinerea, the symptom, pathogen and biology characteristics were studied systematically. The results showed that, Botryotinia was pathogen of konjac Botrytis cinerea, and the conidia germination temperature was from 10 to 30℃. The optimum temperature was 20-25℃. pH of conidia germination was from 4 to 9, and the optimum pH was 5. Conidia could germinate in various nutritive liquid. 2% of sucrose solution was the best, followed by 2% of glucose or konjac glucomannan solution. Lethal temperature of conidia was 57℃ for 5 min. Botrytis cinerea could grow in 4 kinds of medium. PDA was the most appropriate medium for mycelia growth, and PDA plus konjac mannan was the best medium for sporulation. Pathogen could grow and sporulate at 5-30℃ and pH from 4 to 9, and the optimum condition was at 20-25℃ and pH 5.