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中国农学通报 ›› 2014, Vol. 30 ›› Issue (7): 306-308.doi: 10.11924/j.issn.1000-6850.2013-2445

所属专题: 植物保护

• 植物保护 农药 • 上一篇    下一篇

华重楼软腐病的病原鉴定与病害分析

张国辉 任永权 李娇梅 黄同敏 周文湖   

  • 收稿日期:2013-09-12 修回日期:2013-10-31 出版日期:2014-03-05 发布日期:2014-03-05
  • 基金资助:
    黔科合ZY字“独脚莲(重楼)林下规范化种植研究与开发”([2012]3001-8号);贵州省教育厅优秀科研创新团队项目“黔东南生物多样性保护和利用创新团队”(黔教合人才团队字[2013]26)。

Pathogen Identification and Damage Analysis on Soft Rot of Paris polyphylla var. chinensis

  • Received:2013-09-12 Revised:2013-10-31 Online:2014-03-05 Published:2014-03-05

摘要: 华重楼是重要的中药材,研究华重楼软腐病的病原、发生规律和防治方法,为其栽培管理和病害预防提供依据。采用田间调查法观察发病植株的症状,采用组织分离法分离和纯化得到病原菌,通过显微观察法鉴定出其病原菌。华重楼软腐病的发病部位是茎、叶和花,发病后植株弯折,严重腐烂。茎部病斑初期椭圆形,呈水浸状,后期出现小黑点。叶先由叶柄处开始发病,病部布满白色霉状物,后期叶片软腐。花先由花梗和外花被处发病,发病症状和病症与叶类似。病原菌菌丝发达,有分枝、匍匐丝和假根,无隔膜。孢囊梗直立,顶端产生单生的球形孢子囊。华重楼软腐病的病原菌为匍枝根霉(Rhizopus stolonifer),雨后要注意通风排水,并用多菌灵和甲基托布津防治。

关键词: 综合利用, 综合利用

Abstract: The pathogen and prevention of soft rot disease on Paris polyphylla var. chinensis is studied to cultivate this important Chinese medicinal herb. Field investigation was conducted to observe the symptom on the sick plants of P. polyphylla var. chinensis, and tissue separation was conducted to separate and purify the pathogen, and then the pathogen was identified through microscopic observation. The pathogenic sites on soft rot disease on P. polyphylla var. chinensis were stem, leaf and flower, the plant bent and seriously rotted after disease attack. Early stem lesion was oval and water-soaked, and then black dots appeared. The onset of the disease on leaf was petiole, at first petiole was covered by white mold, and the whole leaf was water-soaked later. The onset of the disease on flower was pedicel and then outer perianth lobe, and sign and symptom were similar to those on leaves. Pathogen hyphae without diaphragm developed well, was consisted of branches, creeping silk and rhizoid. Sporangiophore upright stood, and the single spherical sporangium was on the top of sporangiophore. The pathogen of soft rot disease on P. polyphylla var. chinensis was Rhizopus stolonifer. Ventilation and drainage were necessary after rain, and carbendazim and thiophanate methyl were effective pesticides for prevention and cure the soft rot disease on P. polyphylla var. chinensis.