培养基中无机盐浓度及培养方式对栀子组培苗增殖生长的影响

doi:10.11924/j.issn.1000-6850.2013-2944

中国农学通报 ›› 2014, Vol. 30 ›› Issue (13): 256-259.doi: 10.11924/j.issn.1000-6850.2013-2944

培养基中无机盐浓度及培养方式对栀子组培苗增殖生长的影响

魏楠楠李阳朴炫春廉美兰

收稿日期:2013-11-10 修回日期:2013-11-26 出版日期:2014-05-05 发布日期:2014-05-05

Effect of Inorganic Salts Concentrations in Medium and Culture Methods on Proliferation and Growth of Gardenia jasminoides Ellis

Received:2013-11-10 Revised:2013-11-26 Online:2014-05-05 Published:2014-05-05

摘要/Abstract

摘要： 以栀子(Gardenia jasminoides Ellis)组培苗作为试验材料，研究培养基中无机盐浓度及培养方式对栀子组培苗增殖的影响，为栀子的大规模生产和快速繁殖提供理论依据。试验结果表明：MS培养基适合栀子的增殖培养；栀子增殖培养时总氮浓度以60 mmol/L为宜；NO3^-/NH4⁺为40/20有利于栀子不定苗的增殖；反应器间歇浸没培养为最适宜的培养方式。

关键词: 农地流转, 农地流转, 乡村社会经济转型, 非农就业机会

Abstract: The study was conducted to investigate the effect of inorganic salts concentrations and culture methods on the proliferation of Gardenia jasminoides, in order to supply theoretical bases for large-scale and rapid production. Gardenia jasminoides plantlets in vitro were used as experiment materials. The results showed that the standard MS strength (1.0 MS) was optimal. In addition, 60 mmol/L nitrogen and 40 mmol/L NO3^- mixed 20 mmol/L NH4⁺ in medium were the most appropriate for proliferation of Gardenia jasminoides plantlets in vitro. Ebb and flood bioreactor culture was the most suitable culture method.

魏楠楠李阳朴炫春廉美兰. 培养基中无机盐浓度及培养方式对栀子组培苗增殖生长的影响[J]. 中国农学通报, 2014, 30(13): 256-259.

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培养基中无机盐浓度及培养方式对栀子组培苗增殖生长的影响

Effect of Inorganic Salts Concentrations in Medium and Culture Methods on Proliferation and Growth of Gardenia jasminoides Ellis

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