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中国农学通报 ›› 2014, Vol. 30 ›› Issue (11): 1-5.doi: 10.11924/j.issn.1000-6850.2013-3034

所属专题: 生物技术 小麦

• 畜牧 动物医学 蚕 蜂 •    下一篇

纤维素酶对香蕉茎与小麦麸混合青贮效果的影响

詹湉湉 叶杭 张文昌 陈国富 朱小清 庄益芬   

  • 收稿日期:2013-11-19 修回日期:2014-01-06 出版日期:2014-04-15 发布日期:2014-04-15
  • 基金资助:
    国家菌草工程技术研究中心开放基金课题“菌糟饲料化的发酵保存技术研究”(JCJJ13017)。

Effects of Cellulase on Efficiency of Mixed Silage of Banana Stem and Wheat Bran

  • Received:2013-11-19 Revised:2014-01-06 Online:2014-04-15 Published:2014-04-15

摘要: 试验旨在研究添加不同水平纤维素酶对香蕉茎与小麦麸混合青贮品质的影响。在调制香蕉茎与小麦麸质量比为9:1的混合青贮中,设对照组和分别添加纤维素酶0.05 g/kg (WB-CEL1)、0.15 g/kg (WBCEL2)和0.25 g/kg (WB-CEL3)的3个处理组,同时调制香蕉茎单贮。每个处理重复3次。常温下贮存60天,开封后评定青贮的品质。(1)较香蕉茎单贮,混贮对照组的pH、气体损失率(GLR)和中性洗涤纤维(NDF)降低(P<0.01);(2)与混贮对照组相比,3个水平纤维素酶组的干物质(DM)和干物质回收率(DMR)升高、氨态氮(AN/TN)降低(P<0.01);(3)与0.05 g/kg组相比,0.15 g/kg组和0.25 g/kg组的AN/TN降低、水溶性碳水化合物(WSC)升高(P<0.05);(4)与0.15 g/kg组相比,0.25 g/kg组的NDF降低(P<0.01)。表明,香蕉茎与小麦麸混贮优于香蕉茎单贮,在香蕉茎与小麦麸混贮中添加不同水平的纤维素酶均能显著提高青贮品质,以添加0.25 g/kg纤维素酶的效果最好。

关键词: 多克隆抗体, 多克隆抗体

Abstract: The experiment was to study the effect of different levels of cellulase on the quality of silage of the banana stem and wheat bran. The present test set 4 groups in the modulation of banana stem and wheat bran with mass ratio of mixed silage of 9:1, including 1 contrast group (WB) and the other 3 treatment groups adding different levels of cellulase respectively: 0.05 g/kg cellulase (WB-CEL1), 0.15 g/kg cellulase (WB-CEL2) and, 0.25 g/kg cellulase (WB-CEL3). At the same time modulation of banana stem single storage was set (CON). Each treatment was repeated 3 times. After ensilage for 60 days under normal temperature, the four groups were unsealed for assessing the quality of silage. The results were as follows: (1)Compared with the control group, pH, GLR and NDF of the WB group reduced (P<0.01). (2)Compared with the WB group, DM and DMR of three CEL groups increased (P<0.01), AN/TN reduced (P<0.01). (3)Compared with the 0.05 g/kg group, AN/TN of the 0.15 g/kg group and the 0.25 g/kg group reduced (P<0.05), WSC increased (P<0.05). (4) Compared with the 0.15 g/kg group, NDF of the 0.25 g/kg group reduced (P<0.01). This study showed that the mixed silage of banana stem and wheat bran was better than the single storage of the banana stem. The quality of silage could be significantly improved with the different levels of cellulase in banana stem and wheat bran, and adding 0.25 g/kg cellulase was the best.