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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (21): 311-315.doi: 10.11924/j.issn.1000-6850.2013-3094

• 食品 营养 检测 安全 • 上一篇    下一篇

解淀粉芽孢杆菌NCPSJ7抑制青霉病病原菌及防治梨采后青霉病的效果

裘纪莹 黄玲玲 陈蕾蕾 张奇志 王未名 陈相艳   

  • 收稿日期:2013-11-25 修回日期:2013-12-26 出版日期:2014-07-25 发布日期:2014-07-25
  • 基金资助:
    国家自然科学基金青年科学基金项目“生防木霉SMF2产蛋白酶对植物病原真菌的拮抗机理研究”(31100039)；山东省优秀中青年科学家科研奖励基金“拮抗芽孢杆菌NCPSJ7产新型抗菌蛋白的分离纯化及其抑菌机理研究”(BS2013SW039)；山东省农业重大应用技术创新课题“果蔬生物防腐保鲜关键技术研究”(鲁财农指［2012］79号)；国家（中日）国际科技合作专项“水果生物保鲜及内部品质无损检测合作研究”(2013DFG32760)。

Biological Control of Blue Mold Disease on Pear Fruit by Bacillus amyloliquefaciens NCPSJ7

  • Received:2013-11-25 Revised:2013-12-26 Online:2014-07-25 Published:2014-07-25

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摘要： 为了试验广谱抗菌解淀粉芽孢杆菌NCPSJ7抑制青霉病致病菌及防治梨采后青霉病的效果。以NCPSJ7发酵液、发酵上清液、菌悬液及无菌胞外抗菌蛋白为试验材料，分别采用牛津杯法、果实打孔接种法检验NCPSJ7不同处理液对病原菌ACCC37275的平板抑制效果、果体抑制效果，采用果实喷洒防腐试验检验对梨果实的防腐保鲜效果。结果表明，NCPSJ7不同处理液对梨青霉病病原菌ACCC37275均有抑制作用，同时对梨采后青霉病均有不同程度的防治作用。具体地来说，发酵液、发酵上清液、菌悬液等带菌处理液对病原菌ACCC37275的抑制效果较好，而无菌胞外抗菌蛋白对梨果实的防腐保鲜作用较好。所以从实际应用角度考虑，NCPSJ7胞外抗菌蛋白更适用于梨采后青霉病的生物防治。

关键词: 遗传分析, 遗传分析

Abstract: The aim was to estimate the biological control effects of pear blue mold disease and its pathogen by Bacillus amyloliquefaciens NCPSJ7, which displayed a broad antimicrobial spectrum. Fermentation broth, fermentation supernatant, bacterial suspension, and sterile antibacterial protein of strain NCPSJ7 were selected as test samples. The inhibition effects of the test samples on pathogen ACCC37275 were studied using oxford cup diffusion or inoculation pathogen on punched fruit methods. Moreover, a fruit preservation test was adopted to investigate the effects of the test samples on the biological control of pear blue mold disease. It showed that compared with the sterile antibacterial protein, fermentation broth, fermentation supernatant, and bacterial suspension possessed better inhibition effects on the growth of pathogen mycelia, but relatively poorer control effects on pear blue mold disease. Therefore, from the practical point of view, the antibacterial protein secreted by B. amyloliquefaciens NCPSJ7 was more suitable for biological control of blue mold disease on pear fruit.