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中国农学通报 ›› 2014, Vol. 30 ›› Issue (21): 316-320.doi: 10.11924/j.issn.1000-6850.2013-3160

• 食品 营养 检测 安全 • 上一篇    

水果及其制品中果胶不同提取方法的比较

庞荣丽 张巧莲 郭琳琳 谢汉忠 方金豹 李君   

  • 收稿日期:2013-12-03 修回日期:2014-01-03 出版日期:2014-07-25 发布日期:2014-07-25
  • 基金资助:
    农业部农业行业标准制修定项目 “水果及其制品中果胶含量的测定分光光度法” [农财发(2006)64号]。

Comparison of the Different Methods in Extracting Pectin from Fruits and Derived Products

  • Received:2013-12-03 Revised:2014-01-03 Online:2014-07-25 Published:2014-07-25

摘要: 为研究水果及其制品中果胶的提取方法,以橙子、苹果、猕猴桃、草莓酱等为试材,以半乳糖醛酸为标准物质,采用乙醇沉淀、硫酸-咔唑比色法,对水果及其制品中果胶的酸提取方式的提取条件如提取酸种类、提取液温度、提取液酸度、提取时间等进行了研究,并将用酸提取的效果和用碱提取的效果进行分析比较。结果表明,酸提取方式的最佳提取条件为用pH 0.5的硫酸溶液在85℃条件下提取60 min。在此条件下,方法的准确度高,精密度好,符合相关标准要求。通过和碱提取方式的比较发现,原果胶含量高的样品用酸提取比用碱提取效果更好,但香蕉等淀粉含量高的样品因有异常颜色反应而不能采用酸提取的方式。因而确定果胶提取方式为对于酸提取后有异常颜色反应的样品采用碱提取方式,对于原果胶含量较高的样品采用酸提取方式,而对于原果胶含量较低的样品采用酸提取方式和碱提取方式均可。

关键词: 亚细胞定位, 亚细胞定位

Abstract: In order to determine the pectin content in fruits and derived products, using orange, apple, kiwifruit, strawberry jam etc as test material, and galacturonic acid as standard substance, using ethanol precipitation, sulfuric acid- carbazole colorimetry, the conditions of acid extracting pectin from fruits and derived products were studied. The conditions included acid type, extraction solution temperature, extraction solution acidity, extraction time and other conditions. We compared the extraction efficiency of acid extraction and alkaline extraction. The test results showed that the optimum conditions of acid extraction for use of sulfuric acid solution (pH 0.5) extracting for 60 min at 85℃. Under these conditions, the method of high accuracy and precision met the requirements of relevant standards. Through the comparison of the alkali extraction method and found that, for high original pectin samples, with the acid extract was more thorough than the alkali extraction. However, for high starch samples such as banana, acid extraction method was not suitable because of abnormal color reaction. The pectin extraction method was confirmed. For samples with abnormal color reaction, alkaline extraction was used. For samples of the original high pectin, acid extraction was used. For samples with low pectin content, two extraction methods were suitable.