欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (3): 129-138.doi: 10.11924/j.issn.1000-6850.casb2020-0010

所属专题： 生物技术； 小麦

• 生物科学 • 上一篇    下一篇

小麦TaHPPR基因的克隆与表达分析

郝小聪(), 王伟伟, 张风廷, 孙瑞, 房兆峰, 柳珊, 曹志琛, 朱文根, 赵昌平, 汪德州(), 唐益苗()   

  1. 北京市农林科学院杂交小麦工程技术研究中心,北京 100097
  • 收稿日期:2020-07-20 修回日期:2020-10-19 出版日期:2021-01-25 发布日期:2021-01-26
  • 通讯作者: 汪德州,唐益苗
  • 作者简介:郝小聪,女,1995年出生,硕士研究生,研究方向：小麦雄性不育。通信地址：100097 北京市海淀区曙光花园中路9号小麦中心 北京市农林科学院,Tel：15652585689,E-mail： 15652585689@163.com。
  • 基金资助:
    基因组学育种协同创新中心资助项目“重要麦类作物基因组育种”(KJCX201907-2);北京市农林科学院博士后基金“小麦光温敏雄性不育相关基因上游调控基因的筛选及初步分析”(2019-ZZ-005);北京市农林科学院青年科研基金“小麦光温敏雄性不育相关的MYB基因鉴定与功能分析”(QNJJ201805);国家高产转基因重大专项“优质、高产转基因小麦新品种培育”(2016ZX08002003)

TaHPPR Gene in Wheat: Cloning and Expression Analysis

Hao Xiaocong(), Wang Weiwei, Zhang Fengting, Sun Rui, Fang Zhaofeng, Liu Shan, Cao Zhishen, Zhu Wengen, Zhao Changping, Wang Dezhou(), Tang Yimiao()   

  1. Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing 100097
  • Received:2020-07-20 Revised:2020-10-19 Online:2021-01-25 Published:2021-01-26
  • Contact: Wang Dezhou,Tang Yimiao

RichHTML

15

PDF (PC)

224

摘要：

为研究羟基苯丙酮酸还原酶(HPPR)在小麦逆境胁迫中的作用,本研究利用同源克隆法获得1个小麦HPPR基因,命名为TaHPPR,并对其组织表达特性和逆境胁迫表达特性进行了分析。以‘太原806’、‘小白麦’、‘京冬8’及‘京411’为供试材料,用荧光定量方法获得组织表达和逆境胁迫表达数据。序列分析表明：TaHPPR基因含有1个975 bp的完整开放阅读框,编码324个氨基酸;TaHPPR蛋白含有一个NAD-binding domain结构域。系统进化分析表明：TaHPPR基因与二粒小麦处于同一分支,其亲缘关系最近。荧光定量表达分析表明：TaHPPR基因在小麦根、小穗（除去雄蕊）、叶鞘均有表达,其中,根中表达量最高;在低温、干旱、高盐和ABA胁迫处理下TaHPPR基因表达均有所下降;在高抗盐品种‘京冬8’中,TaHPPR基因表达升高,而在敏感品种‘小白麦’和较敏感品种‘京411’中,TaHPPR基因表达受到抑制。亚细胞定位结果显示,TaHPPR蛋白主要在线粒体中表达,过表达TaHPPR基因可能增加小麦的抗盐性。本研究分析了TaHPPR在小麦逆境应答及不同品种中盐胁迫处理下的表达特性,为研究小麦抗逆育种分子机制提供新基因资源和新的思路。

关键词: 小麦, 羟基苯丙酮酸还原酶, 非生物胁迫, 抗盐性, 亚细胞定位

Abstract:

To identify the role of hydroxyphenylpyruvate reductase (HPPR) in wheat stress, this study used homologous cloning to obtain a wheat HPPR gene which named TaHPPR. At the same time, the expressions of HPPR gene in tissues and under stress were analyzed. ‘Taiyuan 806’, ‘Xiaobaimai’, ‘Jingdong 8’ and ‘Jing 411’ were used as materials. We performed RT-qPCR analysis to determine TaHPPR gene expression levels in tissue and under stress. Sequence analysis showed that the TaHPPR gene contained a complete open reading frame of 975 bp, and encoded 324 amino acids. TaHPPR protein contained a NAD-binding domain structure. In addition, phylogenetic analysis indicated that TaHPPR gene was in the same branch of Triticum dicoccoides and their genetic relationships were extremely familiar. Expression profiling revealed that TaHPPR expressed in roots, spikelets (excluding stamens), and leaf sheaths, of which the expression level in roots was the highest. The expression of TaHPPR gene decreased under cold, drought, high salt and ABA stress treatment, In the high-salt resistance variety ‘Jingdong 8’, TaHPPR gene expression increased, while it was suppressed in the sensitive variety ‘Xiaobaimai’ and the moderate sensitive variety ‘Jing411’. The results of subcellular localization show that TaHPPR protein is mainly expressed in mitochondria, and the overexpression of TaHPPR gene may increase the salt tolerance in wheat. This study clarifies the expression characteristics of TaHPPR under adversity stress response and salt stress treatment in different varieties, and provides new gene resources and new ideas for studying the molecular mechanism of wheat resistance breeding.

Key words: wheat, hydroxyphenylpyruvate reductase, abiotic stress, salt resistance, subcellular localization

中图分类号: