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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (21): 224-227.doi: 10.11924/j.issn.1000-6850.2013-3193

所属专题： 油料作物

• 生物技术科学 • 上一篇    下一篇

花生自毒物质降解菌的筛选及其降解效果初步研究

何志刚 汪仁 王秀娟 牛世伟 李波   

  • 收稿日期:2013-12-05 修回日期:2014-03-23 出版日期:2014-07-25 发布日期:2014-07-25
  • 基金资助:
    辽宁省科技攻关重大专项 “花生高产高效栽培技术研究与示范” (2011201030)。

Preliminary Study of Screening of Peanut Autotoxicity Degrading Microorganism and the Degradation Effect

  • Received:2013-12-05 Revised:2014-03-23 Online:2014-07-25 Published:2014-07-25

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摘要： 为了能够有效抑制花生连作障碍中自毒物质对花生的危害，利用选择性培养基筛选用于降解花生自毒物质苯甲酸的菌株，研究其降解与拮抗效果。采用以苯甲酸为唯一碳源，逐渐提高苯甲酸浓度的驯化方法。筛选到3株能够高效降解苯甲酸的菌株，降解率分别为95.32%，91.63%和90.15%，3菌株对花生根系分泌的自毒物质均有较强的降解效果，同时3菌株对花生根腐病菌有较强的拮抗作用，盆栽试验结果表明通过施加菌剂可以有效缓解自毒物质对花生幼苗的抑制作用，增产效果显著。筛选到的3株菌株具有解毒抗菌双重功能，为今后在农业生产中有效利用菌剂进行农作物连作障碍治理和改善土壤环境，提供理论依据。

关键词: 湖南省, 湖南省

Abstract: The aim of the study was to effectively suppress harm to peanuts in the condition of peanut cropping autotoxins. By screening the strains, which could degrade peanut autotoxicity benzoic, we investigated the degradation and antagonistic effect. Using the benzoic acid as sole carbon source, the methods of benzoic acid concentration were increased gradually. three degrading strains were screened, the degradation rate of the benzoic acid were 95.32%, 91.63% and 90.15%, the three strains on peanut root secretion from the autotoxicity had a strong degradation effect, while three strains of Fusarium oxysporumthere is a strong antagonistic. The results of pot experiment showed that by the applying of the three strains could effectively alleviate the inhibitory effect of autotoxicities of peanut seedlings, increase yield significantly. The three strains had the double function of detoxification and antibacterial. They could be effectively used for microfertilizer in the agricultural production of crops in the future and improve the soil environment, providing a theoretical basis.