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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (24): 294-298.doi: 10.11924/j.issn.1000-6850.2014-0347

• 农学 农业基础科学 • 上一篇    下一篇

青稞籽粒中β-葡聚糖在发芽过程中的变化

张伊迪 周选围   

  • 收稿日期:2014-02-17 修回日期:2014-04-18 出版日期:2014-08-25 发布日期:2014-08-25
  • 基金资助:
    西藏圣龙实业有限公司资助 “西藏特有生物资源的开发和利用” (2013 3100 31 001210)。

Analysis of the β-glucan Content Changes in Germination Process of Highland Barley Kernels

  • Received:2014-02-17 Revised:2014-04-18 Online:2014-08-25 Published:2014-08-25

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摘要： 确定青稞的处理条件对发芽率的影响，测定与分析发芽青稞中β-葡聚糖的含量的变化。不同温度和时间条件下，统计青稞籽粒的发芽率，并收集样品；采用苯酚-硫酸法，对不同发芽条件下青稞中的β-葡聚糖的含量进行了测定并分析。27℃浸泡6 h后，在27℃条件下培养15 h发芽率最高，在22℃培养条件下β-葡聚糖含量最高为10.01%；通过方法学考察，含量在0~1.0 mg范围内线性关系良好，回归方程为Y=3.9015X-0.0215，R2=0.9939(n=7)，平均回收率为96.38%，RSD=1.53%(n=5)。以高含量β-葡聚糖为目的青稞加工，可选择在27℃条件下6 h浸泡，22℃培养发芽作为处理条件。

关键词: 苗期效应, 苗期效应

Abstract: In order to investigate the effect of different process conditions on germination rate of highland barley, determine and analyze the content ofβ-glucan in highland barley germination process, in the present study, highland barley germination rate was analyzed under different germination conditions, such as treating temperature and culture time, while various samples were collected. Subsequently highland barleyβ-glucan was determined and analyzed by phenol-sulfate method. The results showed that the best germination rate was obtained under the conditions of soaking for 6 h at 27℃, then culture for 15 h at 27℃. The highest content ofβ- glucan was 10.01% on the conditions of soaking for 6 h at 27℃and culture for 15 h at 22℃. The methodological study showed that theβ-glucan had a good linear relation in the range of 0-1 mg and the regression eguation was Y=3.9015X-0.0215, R=0.9939 (n=7). The average recovery was 96.38% with the RSD of 1.53% (n=5). Therefore, to get the high content of highland barleyβ-glucan, the grains should be treated on the conditions of soaking for 6 h at 27℃and culture for 15 h at 22℃.