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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (35): 107-111.doi: 10.11924/j.issn.1000-6850.2014-0630

所属专题： 生物技术； 水产渔业

• 水产 渔业 • 上一篇    下一篇

不同生长阶段尼罗罗非鱼IGF1基因表达分析

胡一丞,邹芝英,杨弘   

  1. 中国水产科学研究院淡水渔业研究中心,,中国水产科学院究院淡水渔业研究中心
  • 收稿日期:2014-03-11 修回日期:2014-03-11 接受日期:2014-05-19 出版日期:2015-03-18 发布日期:2015-03-18
  • 通讯作者: 杨弘
  • 基金资助:
    现代农业产业技术体系建设专项资金项目“罗非鱼产业技术体系”（No.CARS-49）；现代农业人才支撑计划经费；罗非鱼原良种保种选育项目

The Expression Analysis of IGF1 Gene During Different Developmental Stages in Nile Tilapia

  • Received:2014-03-11 Revised:2014-03-11 Accepted:2014-05-19 Online:2015-03-18 Published:2015-03-18

摘要： 为阐明IGF1基因在罗非鱼生长过程中的表达规律，为罗非鱼的分子选育等工作奠定了理论基础，本实验利用实时荧光定量PCR技术跟踪尼罗罗非鱼(Oreochromis niloticus)在不同生长阶段（0、20、40、60、80、125、145、165、185、205天）肝脏和肌肉胰岛素样生长因子1(IGF1)基因表达的发育性变化。结果表明：实验鱼的体重增长过程可以分为快速生长期和平稳期。在快速生长期时，肝脏IGF1基因表达水平较高，平稳期时较低，肝脏中IGF1基因表达情况与体重增长趋势基本吻合。肌肉中IGF1基因mRNA含量要低得多，而且表达模式与肝脏IGF1基因不相同。研究结果提示：IGF1 mRNA的表达具有明显的时空变化和组织特异性。肝脏中IGF1基因的表达量变化与罗非鱼体重增长趋势一致。肌肉中IGF1表达模式与肝脏中不同，提示肌肉中IGF1的分泌和作用有其自身的特异性。

关键词: 时空变化特征, 时空变化特征

Abstract: To study the expression mechanism of insulin- like growth factor 1(IGF1) gene and lay the theoretical basis for molecular selection of tilapia breeds，IGF1 gene mRNA level was analyzed during different developmental stages of Nile tilapia (Oreochromis niloticus). The expression level of IGF1 gene in liver and muscle were quantified by Real-time PCR on day 0, 20, 40, 60, 80, 125, 145, 165, 185, 205. The results showed that development of body weight in tested fish were divided into different stages: rapid growth stage (RGS) and stationary growth stage (SGS). In RGS, the hepatic gene expression level of IGF1 was higher than that in SGS, sharing the same trend with the tendency of body weight development. In muscle, the IGF1 mRNA level was evidently lower than in liver. In conclusion, IGF1 mRNA level differed with temporal and tissuespecific characteristics. The developmental changes of hepatic IGF1 mRNA might be a key factor to different growth speed in development stages in Nile tilapia. Compared with hepatic pattern, muscular IGF1 gene expression was different, which implied a distinct functional pattern of IGF1 in muscle.