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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (2): 91-96.doi: 10.11924/j.issn.1000-6850.2014-1753

• 水产 渔业 • 上一篇    下一篇

免疫增强剂对仿刺参肠道微生物的影响

樊英,李乐,于晓清,刘恩孚,李天保,叶海斌   

  1. 山东省海洋生物研究院,山东省海洋生物研究院,山东省海洋生物研究院,山东省海洋生物研究院,山东省海洋生物研究院,山东省海洋生物研究院
  • 收稿日期:2014-06-23 修回日期:2015-01-13 接受日期:2014-07-17 出版日期:2015-03-19 发布日期:2015-03-19
  • 通讯作者: 李天保
  • 基金资助:
    山东省现代农业产业技术体系刺参产业创新团队建设项目“病害防控岗位研究”等等

Effect of Immunostimulants on Intestinal Microorganisms of Sea Cucumber, Apostichopus japonicus

  • Received:2014-06-23 Revised:2015-01-13 Accepted:2014-07-17 Online:2015-03-19 Published:2015-03-19

摘要： 为了探讨肠道微生物和免疫增强剂的相互作用、拓展免疫增强剂的评价效果，通过植物源免疫增强剂党参来研究仿刺参肠道微生物的特性及其代谢能力变化。从摄食免疫增强剂党参后的健康仿刺参肠道中分离纯化优势菌株，经16S rDNA进行鉴定；以仿刺参“腐皮综合症”致病菌灿烂弧菌(Vibrio splendidus)为指示菌，采用十字交叉和纸片法进行体外拮抗试验，优势菌株进行生理生化特性分析，肠道内容物进行代谢能力分析。结果表明，筛选优化菌株共18株，系交替假单胞菌属、芽胞杆菌属、希瓦氏菌属、不动杆菌属、弧菌属等不同菌属；拮抗试验筛选优势菌株共4株，通过分泌酶等活性物质产生空间竞争，同时产生抑制灿烂弧菌的胞外产物，发生协同抑菌效果；4株优势菌株具有不同的底物特异性，通过透明圈观察其蛋白酶和淀粉酶活性较高，氧化酶、蔗糖、赖氨酸酶试验表现了不同的生化性质；且经党参投喂后仿刺参肠道微生物菌群代谢能力提高。总结，植物源免疫增强剂党参可调控、优化仿刺参肠道微生物特性，提高肠道代谢力，间接提高机体免疫力。

关键词: 淀粉积累, 淀粉积累

Abstract: In order to study interaction and effect between intestinal microflora and immunostimulants, it was conducted to determine their character and metabolism on intestinal microorganisms of Apostichopus japonicus through Codonopsis. Bacterium was isolated from the intestinal of healthy A. japonicus fed with codonopsis, and identified by 16S DNA. Cross-streak method and paper disk method were used in the antagonism experiment against Vibrio splendidus, and biochemical characteristics were conducted. The results showed that 18 bacteria were from different genera, they were Pseudoalteromonas sp, Bacillus sp, Shewanella sp, Acinetobacter sp, Vibrio sp. The inhibitory test showed that the inhibitory mechanism of 4 superiority strains were competing for living space and producing antibacterial substance. They had different substrate specificity, their protease and amylase activities were high. The oxidase, sucrose and lysine tests showed different biochemical characters. Codonopsis can control and optimize the intestinal function of A. japonicus, improve metabolism and their immunity.