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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (13): 36-42.doi: 10.11924/j.issn.1000-6850.2014-1819

• 林学 园艺 园林 • 上一篇    下一篇

盐胁迫对深色有隔内生真菌D575和D377生长及营养代谢的影响

宋小双,邓勋   

  1. 黑龙江省林科院森林保护研究所,黑龙江省林科院森林保护研究所
  • 收稿日期:2014-07-01 修回日期:2015-03-30 接受日期:2015-02-25 出版日期:2015-06-02 发布日期:2015-06-02
  • 通讯作者: 宋小双
  • 基金资助:
    国家自然科学基金项目“深色有隔内生真菌与外生菌根菌互作对樟子松促生抗逆协同作用机制”(31200484)、“外生菌根菌与木霉互作对针叶苗木促生抗逆作用机制”(31170597)；国家“十二五”科技攻关项目专题“樟子松枯梢病微生物种群生态调控技术”(2012BAD19B0801)；黑龙江省森工总局项目“平贝土传病害生态控制技术”(SGZJY2012016)、“药用植物主要病虫害无公害防治技术”(SGZJY2010014)。

Effects of Salt Stress on the Growth and Nutritional Metabolism of Two Dark Septate Endophyte (DSE) Fungus D575 and D377

  • Received:2014-07-01 Revised:2015-03-30 Accepted:2015-02-25 Online:2015-06-02 Published:2015-06-02

摘要： 为了研究樟子松根部深色有隔内生真菌的耐盐性，笔者采用菌物学、生物化学等方法，研究了樟子松根部深色有隔内生(dark septate endophyte, DSE)真菌D575 和D377 在菌株生长和营养代谢方面对盐胁迫的响应。结果表明，在NaCl 和Na2SO4胁迫条件下，菌株D575 和D377 的生长和营养代谢与盐浓度呈明显的相关性，不同盐浓度条件下菌株生长和营养代谢差异显著(P＜0.05)，在CNaCl≤0.2 mol/L 和CNa2SO4 ≤0.2 mol/L 的低浓度盐胁迫条件下，D377 和D575 菌丝生长和对碳、磷相对利用率受抑制程度均较低，生长速率同对照相比相差不大，随着盐胁迫浓度的增高（CNaCl≥0.3 mol/L 和CNa2SO4 ≥0.3 mol/L），菌株生长受到明显抑制，代谢缓慢。菌株间比较，DSE菌株D575对盐胁迫的耐受性好于菌株D377。

关键词: 黄化曲叶病, 黄化曲叶病, 计算机视觉, 图像处理, RGB, HIS, YCbCr, 特征值提取

Abstract: The paper aims to research the salt resistance of dark septate endophyte (DSE) fungus. Mycology and biochemistry methods were adopted to research the salt tolerance of two dark septate endophyte fungus D377 and D575. The results showed that: under the stresses of NaCl and Na2SO4, the growth and nutritional metabolism were significantly correlated to salt concentrations. Under low salt concentration stress (CNaCl≤0.2 mol/L and CNa2SO4 ≤0.2 mol/L), the inhibitions on strain growth and utilization rates of carbon and phosphorus were lower, and had no significant different compared to control. Under the high salt concentration stress (CNaCl≥0.3 mol/L and CNa2SO4 ≥0.3 mol/L), the inhibitions on strain growth and utilization rates of carbon and phosphorus were higher. DSE strain D575 had a better salt tolerance than D377.