‘惠’苹果自交实生苗叶片高效再生研究

doi:10.11924/j.issn.1000-6850.2014-2119

中国农学通报 ›› 2015, Vol. 31 ›› Issue (1): 83-87.doi: 10.11924/j.issn.1000-6850.2014-2119

所属专题：园艺

‘惠’苹果自交实生苗叶片高效再生研究

郝红梅¹,田义^1,2,张利义^1,2,康国栋^1,2,张彩霞^1,2,丛佩华^1,2

(¹中国农业科学院果树研究所,辽宁兴城 125100；²农业部园艺作物种质资源利用重点实验室,辽宁兴城 125100）

收稿日期:2014-08-04 修回日期:2015-01-06 接受日期:2014-09-30 出版日期:2015-03-18 发布日期:2015-03-18
通讯作者: 郝红梅
基金资助:

基金项目：国家现代农业产业技术体系建设专项资金(CARS-28)；国家科技支撑计划(2013BAD02B01)。

Study on in Vitro Efficient Regeneration System of Apple Seedling Clone from ‘Megumi’

Hao Hongmei¹, Tian Yi^1,2, Zhang Liyi^1,2, Kang Guodong^1,2, Zhang Caixia^1,2, Cong Peihua^1,2

(¹Institute of Pomology of Chinese Academy of Agricultural Sciences, Xingcheng Liaoning 125100;²Key Laboratory of Fruit Germplasm Resourses Utilization, Ministry of Agriculture, Xingcheng Liaoning 125100)

Received:2014-08-04 Revised:2015-01-06 Accepted:2014-09-30 Online:2015-03-18 Published:2015-03-18

摘要/Abstract

摘要：

农杆菌介导的苹果遗传转化是基因功能鉴定和培育抗性苹果新种质的最有效途径之一。苹果自交不亲和的特性导致其基因组高度杂合，为了获得1个杂合度较低的高效再生基因型，对苹果自交亲和品种‘惠’的自交实生苗进行了筛选，选取10个单株上的新梢进行组培，从中筛选出增殖能力最强的单株HZ-5，进一步对影响其叶片不定芽再生的主要因子进行研究。结果表明：MS+2.0 mg/L TDZ+ 0.5 mg/L NAA +30 g/L蔗糖+2.6 g/L胶立得(Gelzan)培养基配方较为适宜；暗培养时间宜控制在14~21天；叶背向下摆放优于叶背向上。当不定芽长至3 cm以上时，将其转到1/2MS+0.3 mg/L IBA+0.2 mg/L IAA+30 g/L蔗糖+6.5 g/L琼脂生根培养基上，生根率达100%。

关键词: 甘肃地方肉牛, 甘肃地方肉牛, LEP 基因, SNPs, 胴体品质, 肉质性状

Abstract: The genetic transformation mediated by Agrabacterium tumefaciens in apple is a vital method in identification functions of genes and cultivation new germplasm. Apple is highly heterozygous due to its self-incompatibility. In order to obtain a genotype with low heterozygous and high regeneration capacity, seedlings from ‘Megumi’ that is self-compatibility are studied in this experiment. The author selected soft cuttings from 10 seedlings and cultivated them in vitro several months. The results showed that HZ-5 exhibited higher proliferative capacity than others. Further, the main factors affecting the regeneration of adventitious buds from leaf explants were studied. The results showed that the appropriate medium was MS 2.0 mg/LTDZ 0.5 mg/LNAA 30 g/L sucrose 2.6 g/LGelzan, and the optimal dark treatment period was 14-21 d；When grown to 3 cm, the adventitious buds were transferred into rooting medium 1/2MS 0.3 mg/L IBA 0.2 mg/LIAA 30 g/Lsucrose 6.5 g/L agar, and the rooting rate was 100%.

郝红梅,田义,张利义,康国栋,张彩霞,丛佩华. ‘惠’苹果自交实生苗叶片高效再生研究[J]. 中国农学通报, 2015, 31(1): 83-87.

Hao Hongmei,Tian Yi,Zhang Liyi,Kang Guodong,Zhang Caixia and Cong Peihua.
Study on in Vitro Efficient Regeneration System of Apple Seedling Clone from ‘Megumi’[J]. Chinese Agricultural Science Bulletin, 2015, 31(1): 83-87.

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‘惠’苹果自交实生苗叶片高效再生研究

Study on in Vitro Efficient Regeneration System of Apple Seedling Clone from ‘Megumi’

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