欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (4): 62-67.doi: 10.11924/j.issn.1000-6850.2014-2125

• 林学 园艺 园林 • 上一篇    下一篇

芋花粉活力测定及最佳贮藏方法的选择

孙亚林1,柯卫东1,黄新芳1,董红霞1,张龙辉2,何燕红2   

  1. (1武汉市蔬菜科学研究所,武汉 430065；2园艺植物生物学教育部重点实验室/华中农业大学园艺林学学院,武汉 430070）
  • 收稿日期:2014-08-05 修回日期:2015-01-29 接受日期:2014-12-03 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 何燕红
  • 基金资助:
    科技部“十二五”国家科技支撑技术计划项目“水生蔬菜高效生产技术研究与示范”(2012BAD-2700)；武汉市青年科技晨光计划“芋杂交技术与遗传规律的研究”(201271031406)。

Determination of Taro Pollen Viability and Choice of the Best Storage Method

Sun Yalin1, Ke Weidong1, Huang Xinfang1, Dong Hongxia1, Zhang Longhui2, He Yanhong2   

  1. (1Wuhan Vegetable Research Institute, Wuhan 430065;2Key Laboratory of Horticultural Plant Biology, Ministry of Education/ College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070)
  • Received:2014-08-05 Revised:2015-01-29 Accepted:2014-12-03 Online:2015-03-20 Published:2015-03-20

摘要： 为确保芋杂交育种实验的有效开展，研究芋花粉活力测定和花粉贮藏的方法。实验结果表明，柱头稀释液培养法和培养基萌发法均能有效测定芋的花粉活力；芋花粉萌发的最佳培养基配方是： 10 mg/L硼酸+0.01%无水氯化钙+5%蔗糖。芋花粉的寿命短，但不同芋品种的花粉寿命不同；芋花粉在15%空气湿度或-20℃干燥条件下可以贮藏相对较长的时间。

关键词: 气候产量, 气候产量

Abstract: The methods to test pollen viability and to store the pollen of taro were studied in order to ensure taro crossbreeding experiment. The results showed that both stigma dilution culture method and medium culture method could be used to test the pollen viability of taro, and the best medium of pollen germination was 10 mg/L boric acid 0.01% calcium chloride anhydrous 5% sucrose. Taro pollen had short life, but different varieties of taro had different pollen lifetimes. Pollen could be stored longer in the 15% air humidity and -20℃ under drying.