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中国农学通报 ›› 2015, Vol. 31 ›› Issue (16): 47-51.doi: 10.11924/j.issn.1000-6850.casb14100095

所属专题: 园艺

• 林学 园艺 园林 • 上一篇    下一篇

氯化钙对低温胁迫下番茄幼苗的影响

廉 洁,谷建田,张喜春   

  1. (北京农学院植物科技学院/北京农业应用技术重点实验室,北京 102206)
  • 收稿日期:2014-10-23 修回日期:2015-05-18 接受日期:2015-03-25 出版日期:2015-07-27 发布日期:2015-07-27
  • 通讯作者: 张喜春
  • 基金资助:
    中国绿色食品协会项目“北京市绿色农业蔬菜新品种选育”(2007163);科技部“中俄主要蔬菜基因资源多样化比较研究”(2011DFR31180-3)。

Effect of Exogenous Calcium Chloride on Tomato Seedlings Under Chilling Injury Stress

Lian Jie, Gu Jiantian, Zhang Xichun   

  1. (College of Plant Science and Technology, Beijing University of Agriculture/Beijing Key Laboratory of New Technology in Agricultural Application, Beijing 102206)
  • Received:2014-10-23 Revised:2015-05-18 Accepted:2015-03-25 Online:2015-07-27 Published:2015-07-27

摘要: 为探究氯化钙如何影响番茄冷胁迫过程,得出氯化钙提高番茄幼苗耐冷性机制,进行了冷害指数调查、生理指标测定。使用光照培养箱低温处理耐冷型‘O-33-1’和冷敏感型‘NY2000’,依照氯化钙处理浓度的不同记为CK、T1、T2、T3,对不同处理调查冷害指数,测定叶绿素含量,测定丙二醛(MDA)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性。研究表明,冷胁迫外源喷施氯化钙时,‘O-33-1’冷害指数、MDA含量、POD含量先下降后升高,叶绿素a含量、叶绿素b含量、SOD活性先升高后下降;‘NY2000’冷害指数先下降后上升,SOD活性先升高后下降,MDA含量不断升高,叶绿素a含量、叶绿素b含量基本不变,POD则表现得没有规律。不同品种响应氯化钙提高耐冷性表现出差异;低温胁迫下,叶绿素的降解受到抑制从而提高了番茄幼苗耐冷性;耐冷性的提高与外源氯化钙导致抗氧化酶活性提高有关。

关键词: 政策建议, 政策建议

Abstract: This experiment aimed to study the role of calcium chloride in improving the chilling tolerance mechanism of tomato seedlings. The author conducted the chilling injury index survey and the measurement of physiological indexes. The materials, which were cold-resistant ‘O-33-1’ and cold-sensitive ‘NY2000’, were treated with light culture box for low temperature treatment. The treatments were named CK, T1, T2, T3 according to different concentrations of calcium chloride. Cold damage index, Chl content, MDA, POD, SOD were measured. When applied with calcium chloride, the cold damage index, MDA and POD of ‘O-33-1’ decreased first and then increased, Chla, Chlb and SOD increased first and then decreased. The cold damage index of ‘NY2000’ decreased first and then increased, SOD increased first and then decreased, MDA content kept an increasing trend, Chla and Chlb were stable. Different tomato varieties had different responses toward exogenous calcium chloride treatments. Under chilling stress, exogenous calcium chloride treatments could protect the stabilization of membrane via enhancing the antioxidant enzymes activities, thus enhance chilling tolerance.