桑树DNA连接酶Ⅰ基因MuLigI的获取和序列分析

doi:10.11924/j.issn.1000-6850.casb14120166

中国农学通报 ›› 2015, Vol. 31 ›› Issue (8): 113-117.doi: 10.11924/j.issn.1000-6850.casb14120166

所属专题：生物技术；园艺

桑树DNA连接酶Ⅰ基因MuLigI的获取和序列分析

王桃妮,孔卫青,杨金宏

(安康学院陕西省蚕桑重点实验室,陕西安康 725000）

收稿日期:2014-12-24 修回日期:2015-01-06 接受日期:2015-01-07 出版日期:2015-04-07 发布日期:2015-04-07
通讯作者: 孔卫青＊
基金资助:
陕西省青年科技新星项目“桑树类病毒在非自然宿主中的复制潜能研究”(2013KJXX-96)；大学生创新创业训练计划项目“桑花叶萎缩类病毒与桑树互作蛋白的筛选研究”(201211397004)。

Acquisition and Sequence Analysis of MuLigⅠ Gene from Mulberry (Morus alba L.)

Wang Taoni, Kong Weiqing, Yang Jinhong

(The Key Sericultural Laboratory of Shaanxi, Ankang University, Ankang Shaanxi 725000)

Received:2014-12-24 Revised:2015-01-06 Accepted:2015-01-07 Online:2015-04-07 Published:2015-04-07

摘要/Abstract

摘要： 为进一步研究DNA连接酶Ⅰ在类病毒复制中的作用，以感染桑花叶萎缩类病毒的桑树叶片为材料，通过在同源基因保守区设计简并引物进行PCR扩增和cDNA末端扩增，获取桑树DNA 连接酶Ⅰ基因。结果获得桑树DNA连接酶Ⅰ基因MuLigI长2730 bp的mRNA序列，基因编码区长2367 bp，编码788个氨基酸，含典型的DBD结构域(173~351 aa)和CD催化结构域(405~771 aa)，N端含有核定位(NLS)和线粒体定位信号(MLS)。研究所得桑树DNA连接酶Ⅰ基因具有保守的结构域和活性位点，可能和番茄的DNA连接酶Ⅰ基因一样，参与了桑花叶萎缩类病毒的复制过程。

关键词: 干沟, 干沟, DEM, 戈壁, 集水区

Abstract: For further study on the role of DNA ligase Ⅰ in the replication of viroid, the DNA ligase Ⅰ gene was obtained from MMDVd-infected mulberry leaf. The gene was amplified by degenerate primer designed in the conserved domain according to the homologous genes and RACE methods. The results showed that the mRNA sequence of MuLig Ⅰ was 2 730 bp in length. The open reading frame (ORF) was 2367 bp and encoding 788 amino acid residues. There were conserved DBD domain (173-351 aa), catalytic domain (405-771 aa), and mitochondrion and nuclear location signal (MLS and NLS) in MuLigⅠ. The MuLig Ⅰ gene in this study contained conserved structural domain and active site, and like the DNA ligase Ⅰ gene of tomato, might be involved in the replication of MMDVd.

王桃妮,孔卫青,杨金宏. 桑树DNA连接酶Ⅰ基因MuLigI的获取和序列分析[J]. 中国农学通报, 2015, 31(8): 113-117.

Wang Taoni,Kong Weiqing and Yang Jinhong. Acquisition and Sequence Analysis of MuLigⅠ Gene from Mulberry (Morus alba L.)[J]. Chinese Agricultural Science Bulletin, 2015, 31(8): 113-117.

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桑树DNA连接酶Ⅰ基因MuLigI的获取和序列分析

Acquisition and Sequence Analysis of MuLigⅠ Gene from Mulberry (Morus alba L.)

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