叶用莴苣2个Hsp70基因与其抽薹的相关性分析

doi:10.11924/j.issn.1000-6850.casb14120178

中国农学通报 ›› 2015, Vol. 31 ›› Issue (10): 58-62.doi: 10.11924/j.issn.1000-6850.casb14120178

所属专题：生物技术；园艺

叶用莴苣2个Hsp70基因与其抽薹的相关性分析

李婷,韩莹琰,范双喜,赵真真

(北京农学院植物科学技术学院,北京 102206）

收稿日期:2014-12-26 修回日期:2015-03-04 接受日期:2015-01-20 出版日期:2015-05-05 发布日期:2015-05-05
通讯作者: 范双喜
基金资助:
国家自然科学基金项目“叶用莴苣高温抽薹相关蛋白的鉴定及其互作分析”(31372057)；国家自然科学基金项目“叶用莴苣高温胁迫响应因子LsHsp70的功能研究”(31401883)；现代农业产业技术体系建设专项“北京市叶类蔬菜创新团队”(blvt-02)；北京农学院促进人才培养综合改革专项计划“叶用莴苣高温胁迫中LsHsp70家族基因的表达模式研究”(BNRC&GG201411)；北京市农业科技项目“生菜耐热新品种繁育及安全生菜技术实验示范项目资助”(20140134)；科研基地建设-科技创新平台“蔬菜创新平台建设”(PXM2014_014207_000028)。

Correlation Analysis of Bolting and Two Hsp70 Genes in Lactuca sativa L.

Li Ting, Han Yingyan, Fan Shuangxi, Zhao Zhenzhen

(Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206)

Received:2014-12-26 Revised:2015-03-04 Accepted:2015-01-20 Online:2015-05-05 Published:2015-05-05

摘要/Abstract

摘要： 为探究叶用莴苣2个Hsp70基因LsHsp70-2711和LsHsp70-3711与叶用莴苣的抽薹是否相关，采用实时荧光定量PCR技术(RT-PCR)，以2个品种的叶片为试材，分析这2个基因在耐热型品种G-S59和热敏型品种P-S11 2个叶用莴苣品种抽薹前后的相对表达量。研究结果表明：首先，这2个Hsp70基因在抽薹前后的表达量表现出显著差异，所以推测与叶用莴苣的抽薹性具有一定关联；其次，LsHsp70-2711基因在2个叶用莴苣品种中的表达趋势均为抽薹前显著高于抽薹后，且在相同处理下G-S59中的表达量又明显高于P-S11中的表达量，LsHsp70-3711基因与LsHsp70-2711基因在2个品种中的表达情况相反。叶用莴苣热激蛋白基因LsHsp70-2711和LsHsp70-3711除与叶用莴苣的耐热性相关外，还与其抽薹性具有一定相关性。

关键词: 病程相关蛋白, 病程相关蛋白

Abstract: Based on a heat-resistant type G-S59 and a heat-sensitive type P-S11, in this study, we analyzed the relationship between bolting and two heat shock protein genes in Lactuca sativa L.(LsHsp70-2711 gene and LsHsp70-3711 gene) with the help of real-time quantitative PCR (qRT-PCR). The result showed that the expression of the two Hsp70 genes in lettuce varied significantly before and after bolting, based on which we could conjecture that there was a relationship between bolting and the two Hsp70 genes in lettuce. In addition, in either type, the relative expression of LsHsp70-2711 after bolting was evidently higher than that beforehand. Moreover, when under the same treatment, the expression of LsHsp70-2711 gene in heat-resistant type G-S59 was much higher than that in heat-sensitive type P-S11 while the expression of LsHsp70-3711 gene had the opposite result. Therefore we deduced that the two Hsp70 genes not only had correlation with heat-resistance in lettuce, but also had some extent relationships with bolting.

李婷,韩莹琰,范双喜,赵真真. 叶用莴苣2个Hsp70基因与其抽薹的相关性分析[J]. 中国农学通报, 2015, 31(10): 58-62.

Li Ting,Han Yingyan,Fan Shuangxi and Zhao Zhenzhen. Correlation Analysis of Bolting and Two Hsp70 Genes in Lactuca sativa L.[J]. Chinese Agricultural Science Bulletin, 2015, 31(10): 58-62.

参考文献

[1] Vanisree Mulabagal, Mathieu Ngouajio, Ajay Nair, et al. In Vitro Evaluation of Red and Green Lettuce (Lactuca sativa) for Functional Food Properties[J]. Food Chemistry,2010,118:300-306.
[2] 许俊强,汤青林,宋明,等.蔬菜抽薹的遗传规律及机理研究[J].长江蔬菜,2011(8):7-10.
[3] 谷建田,范双喜,张喜春,等.结球莴苣耐热性鉴定方法的研究[J].华北农学报,2006,21(s):99-103.
[4] 陈青君,韩莹琰,谷建田,等.叶用莴苣种质资源的主要农艺性状鉴定与耐热性评价[J].中国蔬菜,2011(20):20-27.
[5] 龚繁荣.自然高温下耐热散叶莴苣品种的筛选[J].上海,上海农学院学报,1998,16(3):227-231.
[6] 齐妍,徐兆师,李盼松,等.植物热激蛋白70的分子作用机理及其利用研究进展[J].植物遗传资源学报2013,14(3):507-511.
[7] Feder M E, Hofmann G E. Heat-shock Proteins Molecular Chaperones and the Stress Response[J]. Annu Rev 465 Physiol,1999,61(1):243-282.
[8] 王彦波,周绪霞,许悻荣.热应激蛋白70的研究进展[J].免疫学杂志,2003,19(3):79-82.
[9] Atsushi Kobiyama, Shohei Tanaka, Yutaka Kaneko, et al. Temperature Tolerance and Expression of Heat Shock Protein 70 in the Toxic Dinoflagellate Alexandrium tamarense (Dinophyceae)[J]. Harmful Alage,2010,9:180-185.
[10] Sung D Y, Guy C. Physiological and Molecular Assessment of Altered Expressionof Hsc70-1 in Arabidopsis. Evidence for pleior topic consequences[J]. Plant Physiol,2003,132:979-987.
[11] Latijnhouwers M, Xu X M, Mller S G.. Arabidopsis stromal 70 kDa Heat Shock Proteins are Essential for Chloroplast Development[J]. Biological Sciences,2010,232(3):567-578.
[12] 王勇裴.水稻热激蛋白(Hsp)的鉴定及其Hsp70家族的功能分析[D].福建:福建农林大学,2012:1-76.
[13] Boston R S, Viitanen P V, Vierling E. Molecular chaperones and protein folding in plant[J]. Plant Mol Biol,32(1-2):191-222.
[14] 肖艳萍,宫伟娜,万方浩,等.紫茎泽兰热激蛋白70基因的克隆与序列分析[J].中国农业科技导报,2010,12(1):111-117.
[15] 安艳秋,蔺瑞明,芦冯晶,等.小麦热激蛋白基因TaHSP70克隆及其在植物防卫和抗逆反应中的表达分析[J].分子植物育种,2011,9(4):402-409.
[16] 李婷,韩莹琰,范双喜,等.叶用莴苣LsHsp70基因的克隆及表达分析[J].园艺学报,2013:5-10.
[17] Currey Christopher J, Erwin John E. Foliar Applications of Plant Growth Regulators Affect Stem Elongation and Branching of 11 Kalanchoe Species[J]. Hort Technology,2012,22(3):338-344.
[18] Gibson J L, Whipker B E. Efficacy of plant growth regulators on the growth of vigorous Osteospermum cultivars[J]. HortTechnology,2003,13:132-135.
[19] 刘慧,郝敬虹,韩莹琰,等.高温诱导叶用莴苣抽薹过程中内源激素含量变化分析[J].中国农学通报,2014,30(25):97-103.
[20] 王涛,田雪瑶,谢寅峰,等.植物耐热性研究进展[J].云南农业大学学报,2013,28(5):719-726.

叶用莴苣2个Hsp70基因与其抽薹的相关性分析

Correlation Analysis of Bolting and Two Hsp70 Genes in Lactuca sativa L.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们