低温应激下吉富罗非鱼生化指标与氨氮排泄水平的变化

doi:10.11924/j.issn.1000-6850.casb15040160

中国农学通报 ›› 2015, Vol. 31 ›› Issue (26): 35-39.doi: 10.11924/j.issn.1000-6850.casb15040160

所属专题：水产渔业

低温应激下吉富罗非鱼生化指标与氨氮排泄水平的变化

王振华,宋红桥,吴凡,吴锦婷

(中国水产科学研究院渔业机械仪器研究所/农业部渔业装备与工程技术重点实验室,上海 200092）

收稿日期:2015-04-21 修回日期:2015-08-07 接受日期:2015-07-22 出版日期:2015-09-23 发布日期:2015-09-23
通讯作者: 王振华
基金资助:
国家科技支撑计划课题“淡水水产品保活冷鲜冷链物流关键技术研发”(2012BAD38B04)。

Effects of Cold Stress on Biochemical Parameters and Ammonia Nitrogen Excretion in GIFT Strain of Nile Tilapia (Oreochromis niloticus)

Wang Zhenhua, Song Hongqiao, Wu Fan, Wu Jinting

(Fishery Machinery and Instrument Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of Fishery Equipment and Engineering, Ministry of Agriculture, Shanghai 200092)

Received:2015-04-21 Revised:2015-08-07 Accepted:2015-07-22 Online:2015-09-23 Published:2015-09-23

摘要/Abstract

摘要： 为研究低温应激对吉富罗非鱼(GIFT，Oreochromis niloticus)生化指标与氨氮排泄水平的影响，选取平均体重(232±10) g的吉富罗非鱼作为试验对象，降温速度1℃/h，设定(24.5±0.2)℃对照组和低温(14.3±0.8)℃冷应激试验组，每组设定3个平行组，于试验第0、4.5和24 h时统计试验鱼成活率，测定试验鱼生化指标及水体pH、氨氮含量。结果表明，水温降至应激温度的第0 h，试验组罗非鱼血糖、肌糖原、乳酸水平均有上升，其中肌糖原上升显著。水中氨氮水平也有所增加，但不显著。该应激影响在24 h后通过罗非鱼自身调整消除，各生化指标最终趋于稳定。因此认为在本试验条件下，在该降温速度和暂养温度下短时间内暂养200 g左右的罗非鱼是安全的，对鱼体健康并无显著影响。

关键词: 大风, 大风, 概念模型, 环流形势

Abstract: To study the effect of cold stress on biochemical parameters and ammonia nitrogen excretion of GIFT strain of Nile tilapia (Oreochromis niloticus), GIFT strain of Nile tilapia with an average weight of (232±10) g were cultured in water with temperature decrease speed of 1.0℃/h. The fish were randomly divided into two groups: a control group reared at water temperature of (24.5±0.2)℃ and a treatment group reared at water temperature of (14.3±0.8)℃. The experiments were repeated three times. Biochemical parameters and survival rate of the fish and pH , concentration of ammonia nitrogen in water were tested at 0, 4.5 and 24 h. The results showed that the concentration of blood glucose, muscle glycogen and lactic acid increased in the treatment group, compared with those in the control group at 0 h. Concentration of ammonia nitrogen in water also increased. However, the effect of cold stress on the fish decreased after 24 h. The results indicated that it might be safe to temporarily culture tilapia weighted around 200 g at the above temperature and temperature decrease speed.

王振华,宋红桥,吴凡,吴锦婷. 低温应激下吉富罗非鱼生化指标与氨氮排泄水平的变化[J]. 中国农学通报, 2015, 31(26): 35-39.

Wang Zhenhua,Song Hongqiao,Wu Fan and Wu Jinting. Effects of Cold Stress on Biochemical Parameters and Ammonia Nitrogen Excretion in GIFT Strain of Nile Tilapia (Oreochromis niloticus)[J]. Chinese Agricultural Science Bulletin, 2015, 31(26): 35-39.

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低温应激下吉富罗非鱼生化指标与氨氮排泄水平的变化

Effects of Cold Stress on Biochemical Parameters and Ammonia Nitrogen Excretion in GIFT Strain of Nile Tilapia (Oreochromis niloticus)

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