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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (33): 192-195.doi: 10.11924/j.issn.1000-6850.casb15070026

所属专题： 生物技术； 小麦

• 生物技术科学 • 上一篇    下一篇

山西小麦品种(系)多酚氧化酶基因的等位变异分布

田志刚   

  1. 山西省科学技术情报研究所
  • 收稿日期:2015-07-05 修回日期:2015-08-24 接受日期:2015-09-21 出版日期:2015-11-26 发布日期:2015-11-26
  • 通讯作者: 田志刚
  • 基金资助:
    山西省农科院院攻关“山西省冬小麦品种旗叶光能转化特性研究”(2013YGG54)。

Allelic Variation of the Polyphenol Oxidase Genes in Shanxi Wheat Varieties (Lines)

  • Received:2015-07-05 Revised:2015-08-24 Accepted:2015-09-21 Online:2015-11-26 Published:2015-11-26

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摘要： 为了明确山西小麦品种（系）多酚氧化酶(PPO)基因的等位变异分布，选育PPO活性低的小麦品种。利用PPO基因的功能性标记PPO16、PPO18 和PPO 29 对121 份山西小麦品种（系）进行分析。结果表明：Ppo-A1a 和Ppo-A1b 基因型的品种（系）分别为56 份和65 份，频率分别为46.3%和53.7%；Ppo-D1a和Ppo-D1b 基因型的品种（系）分别为50 份和71 份，频率分别为41.3%和58.7%；两基因组合类型Ppo-A1a/Ppo-D1a、Ppo-A1a/Ppo-D1b、Ppo-A1b/Ppo-D1a 和Ppo-A1b/Ppo-D1b 的小麦品种（品系）分别有43 份、50 份、37 份和56 份，分布频率分别为35.5%、41.3%、30.6%和46.3%。本研究在山西小麦品种（系）中检测出37 份低PPO活性基因Ppo-A1b/Ppo-D1a 组合类型的品种（系），为常规育种获得低PPO活性的小麦品种提供依据。

关键词: 海上大风, 海上大风, 数值模拟, 冷锋过境, 动量下传

Abstract: In order to clarify the allelic variation of polyphenol oxidase (PPO) in Shanxi wheat varieties (lines), and select and breed the wheat varieties with low activity of PPO, a total of 121 Shanxi wheat varieties (lines) were tested with the functional markers PPO16, PPO18 and PPO29 of PPO. The results showed that the wheat varieties (lines) with Ppo- A1a genotype and Ppo- A1b had 56 and 65 accessions, respectively, and the frequencies were 46.3% and 53.7%, respectively. The wheat varieties (lines) with Ppo-D1a genotype and Ppo-D1b had 50 and 71 accessions, respectively, and the frequencies were 41.3% and 58.7%, respectively. The wheat varieties (lines) with two-genotype combination types Ppo-A1a/Ppo-D1a, Ppo-A1a/Ppo-D1b, Ppo-A1b/ Ppo-D1a, Ppo-A1b/Ppo-D1b had 43, 50, 37, 56 accessions, respectively, and the frequency distributions were 35.5%, 41.3%, 30.6% and 46.3%, respectively. 37 accessions of the wheat varieties with low activity of PPO (Ppo-A1b/Ppo-D1a) were detected, which provided the basis for obtaining the wheat varieties with low activity of PPO in conventional breeding.