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中国农学通报 ›› 2015, Vol. 31 ›› Issue (35): 110-115.doi: 10.11924/j.issn.1000-6850.casb15070108

所属专题: 园艺

• 食品 营养 检测 安全 • 上一篇    下一篇

螺虫乙酯在黄瓜及其土壤中残留量检测方法及残留规律研究

吴育佳,杨仁斌,聂红英   

  1. 湖南农业大学农业环境保护研究所,湖南农业大学农业环境保护研究所,湖南农业大学农业环境保护研究所
  • 收稿日期:2015-07-19 修回日期:2015-11-20 接受日期:2015-09-15 出版日期:2015-12-18 发布日期:2015-12-18
  • 通讯作者: 杨仁斌
  • 基金资助:
    农业部农药残留资助基金项目“螺虫乙酯在黄瓜上的安全性评价研究”(2012P091)。

Study on the residue of ethyl acetate in cucumber and soil by HPLC

吴育佳,杨仁斌 and 聂红英   

  • Received:2015-07-19 Revised:2015-11-20 Accepted:2015-09-15 Online:2015-12-18 Published:2015-12-18

摘要: [目的]本文旨在研究黄瓜和土壤中螺虫乙酯残留量的高效液相色谱测定方法,并于2014年用该方法测定湖南、浙江和广东三地螺虫乙酯在黄瓜及其土壤上的消解动态和残留规律试验研究的样品。[方法]样品用乙腈提取,高效液相色谱紫外检测器测定。[结果]结果表明:当添加螺虫乙酯在黄瓜及其土壤中的浓度为0.05mg/kg~1.0mg/kg时,平均添加回收率分别为95.75%~98.91%、89.37%~99.78%;变异系数分别为1.26%~2.89%、1.62%~1.99%,该方法对黄瓜和土壤中螺虫乙酯的最低检出浓度为 0.025mg/kg;在自然环境条件下,螺虫乙酯在湖南、浙江和广州三地的黄瓜和土壤中的半衰期分别为2.67~8.82d,1.95~6.19d。[结论]按推荐使用剂量对黄瓜施药,末次施药后第5天螺虫乙酯在三地的黄瓜和土壤中的残留量均未检出,黄瓜中螺虫乙酯残留量均低于瓜果类的MRL值(0.2mg/kg)。

关键词: 降水再分配, 降水再分配, 物候期, 杜仲人工林

Abstract: This paper aimed to study the determination method of spirotetramat residue in cucumber and soil by high performance liquid chromatography, and the degradation dynamics and residue rule were analyzed by this method in Hunan, Zhejiang and Guangdong in 2014. The samples were extracted by acetonitrile and detected with HPLC UV-detector. The results showed that, when the adding concentration of spirotetramat was 0.05-1.0 mg/kg in cucumber and soil, the average spiked recoveries were 95.75%-98.91% and 89.37%-99.78%, the coefficients of variation were 1.26%-2.89% and 1.62%-1.99%, and the minimum detectable concentration by this method was 0.025 mg/kg. The half-life period of spirotetramat in cucumber and soil in Hunan, Zhejiang and Guangzhou was 2.67-8.82 d and 1.95-6.19 d respectively under natural environmental condition. Spirotetramat could not be detected in cucumber and soil after 5 days of the last application with recommended dosage, and the residue quantity of spirotetramat was below the MRL value of melon and fruit (0.2 mg/kg).

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