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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (35): 97-103.doi: 10.11924/j.issn.1000-6850.casb15070162

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

一种产中性蛋白酶的凝结芽孢杆菌Liu-g1活菌制剂的制备方法

郭茜,张红星,谢远红,熊丽霞,刘慧,连正兴,孔保华   

  1. 北京农学院,北京农学院,北京农学院,北京农学院,北京农学院,中国农业大学,东北农业大学
  • 收稿日期:2015-07-28 修回日期:2015-09-16 接受日期:2015-09-16 出版日期:2015-12-18 发布日期:2015-12-18
  • 通讯作者: 刘慧
  • 基金资助:
    国家“十二五”科技支撑计划项目“动物源食品HACCP体系建设及致病菌高通量检测技术”(2012BAD28B02-01)；转基因生物新品种培育重大专项“抗病转基因羊新品种培育”(2014ZX08008-005)；北京市属高等学校高层次人才引进与培养项目“天然乳酸菌素对畜产品中重要致病菌的抑菌机制及作用研究”(CIT&TCD20140315)。

Preparation method of producing neutral protease by a living bacteria bacillus coagulans Liu-g1

  • Received:2015-07-28 Revised:2015-09-16 Accepted:2015-09-16 Online:2015-12-18 Published:2015-12-18

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摘要： 为提高发酵液中产中性蛋白酶凝结芽孢杆菌Liu-g1 的活菌数量，采用单因素和正交实验研究不同培养基配方和不同发酵条件对凝结芽孢杆菌Liu-g1 活菌数量的影响。结果表明：优化培养基配方为大豆粕1%、玉米淀粉1%、碳酸钙0.2%。选用最优培养基配方，在发酵温度为45℃、接种量2%、装液量50 mL、发酵液pH 7.5、发酵时间为48 h、发酵转速为180 r/min时，发酵剂活菌数量最高，为9.53×109 CFU/mL。在此优化条件下，发酵剂的活菌数量是优化前的28.88倍。,发酵剂的活菌数量是优化前的28.88倍。

关键词: 农田边界, 农田边界, 生态服务, 农田边界缓冲带, 生物多样性保护, 生境提升, 可持续农业

Abstract: In order to increase the number of viable bacteria of Bacillus coagulans Liu-g1 in fermentation liquid for producing protease, through single factor and orthogonal experiments, the authors studied the effects of different culture media and fermentation conditions on the number of Bacillus coagulans Liu-g1 viable bacteria. The results showed that: the optimal culture medium formula was soybean meal 1%, corn starch 1%, calcium carbonate 0.2%. With the optimal formula of culture medium, under the fermentation temperature of 45℃, inoculation quantity of 2%, the volume of liquid of 50 mL, pH 7.5, fermentation time of 48 h, the speed of fermentation of 180 r/min, the highest number of viable bacteria of fermentation agent could be obtained, which was 9.53×109 CFU/mL. Under this condition, the number of viable bacteria of fermentation agent was 28.88 times as much as that before optimization.