欢迎访问《中国农学通报》,

中国农学通报 ›› 2016, Vol. 32 ›› Issue (3): 91-99.doi: 10.11924/j.issn.1000-6850.casb15100087

所属专题: 玉米

• 生物技术科学 • 上一篇    下一篇

甘肃部分玉米自交系SSR遗传多样性分析

李世风,步迅,梁水美,刘艳艳,闫治斌,鲁守平,郭庆法,张全芳   

  1. 甘肃省敦煌种业股份有限公司,山东省农业科学院生物技术研究中心/山东省作物遗传改良与生态生理重点实验室,山东省农业科学院生物技术研究中心/山东省作物遗传改良与生态生理重点实验室,山东省农业科学院生物技术研究中心/山东省作物遗传改良与生态生理重点实验室,甘肃省敦煌种业股份有限公司,山东省农业科学院玉米研究所/小麦玉米国家工程实验室/农业部黄淮海北部玉米生物学与遗传育种重点实验室,山东省农业科学院玉米研究所/小麦玉米国家工程实验室/农业部黄淮海北部玉米生物学与遗传育种重点实验室,山东省农业科学院生物技术研究中心/山东省作物遗传改良与生态生理重点实验室
  • 收稿日期:2015-10-21 修回日期:2015-12-25 接受日期:2015-11-24 出版日期:2016-01-29 发布日期:2016-01-29
  • 通讯作者: 张全芳
  • 基金资助:
    国家玉米产业体系“现代玉米产业技术体系建设”(CARS-02-06);2014 山东省农业良种工程项目“玉米种质重要农艺性状评价、分子标记及优异种质创新”;甘肃省玉米生物育种与种子繁育重点实验室“甘肃省玉米育繁重点实验室”(145RTSF011)。

SSR Genetic Diversity Analysis of Part of Maize Inbred Lines in Gansu Province

  • Received:2015-10-21 Revised:2015-12-25 Accepted:2015-11-24 Online:2016-01-29 Published:2016-01-29

摘要: 分析甘肃省部分骨干玉米自交系的遗传多样性并划分群体结构。利用全自动DNA分析仪荧光SSR-PCR技术和66 对核心SSR引物研究了148 份玉米(Zea mays L.)自交系的遗传多样性,并分别使用模型结构聚类和遗传距离聚类法进行群体结构分析。在148 份自交系中,66 对SSR标记共检出279 个等位变异,每对引物检测到等位基因2~8 个,平均4.2273 个。多态性信息量(PIC)和标记指数(MI)的平均值分别为0.4879 和2.1808。模型结构聚类将148 份自交系分为SS 群和NSS群;遗传距离聚类分为5 个类群,根据常规测验种自交系可将5 个类群归并为SS 群和NSS群。2 种聚类方法所得结果与材料的系谱信息基本一致。甘肃省部分玉米自交系在育种实践中正在向2 个相对独立的优势类群转化,2 个类群之间的遗传多样性水平存在一定的差异。

关键词: 汛期暴雨, 汛期暴雨, 大气环流, 暴雨分型, 低涡

Abstract: The study aims to analyze the genetic diversity and divide the population structure of some maize inbred lines in Gansu Province. Genetic diversity of 148 maize inbred lines was analyzed with 66 pairs of core SSR primers and fluorescent SSR-PCR technology in automatic DNA analyzer. The population structure was analyzed by distance-based cluster and model-based cluster methods. Totally in the 148 lines, 279 alleles were detected with an average of 4.2273, ranged from 2 to 8 per locus with 66 pairs of SSR primers. The average polymorphism information content (PIC) and marker index (MI) were 0.4879 and 2.1808, respectively. All the 148 inbred lines were grouped into NSS group and SS group by model-based cluster method, and were divided into five groups by distance-based cluster method. These 5 groups could be incorporated into SS group and NSS group according to the commonly used test inbred lines. Results showed that the clustering groups were consistent with the basic materials of pedigree information. In conclusion, part of maize inbred lines in Gansu Province were transforming into two relatively independent groups in breeding practice, the genetic diversity level between the two groups had certain differences.