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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (33): 151-156.doi: 10.11924/j.issn.1000-6850.casb16040018

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

阿魏侧耳(Pleuratus ferulae)产漆酶条件的优化及其酶学性质研究

金晓婷,张志勇,杨 栋,余向阳   

  1. 南京财经大学食品科学与工程学院,江苏省农业科学院食品质量安全与检测研究所,江苏省农业科学院食品质量安全与检测研究所,江苏省农业科学院食品质量安全与检测研究所
  • 收稿日期:2016-04-05 修回日期:2016-10-25 接受日期:2016-05-26 出版日期:2016-11-25 发布日期:2016-11-25
  • 通讯作者: 余向阳
  • 基金资助:
    国家自然科学基金项目“食用菌漆酶调控其体内残留农药代谢降解的分子机制研究”(31471797)。

Optimization of Laccase Production and Its Enzymatic Properties of Pleuratus ferulae

  • Received:2016-04-05 Revised:2016-10-25 Accepted:2016-05-26 Online:2016-11-25 Published:2016-11-25

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摘要： 为获得酶活较高的酶液用于农药污染的修复，探讨阿魏侧耳(Pleuratus ferulae)产漆酶的条件。对碳源、氮源、pH诱导剂和金属离子这5 个培养条件进行优化，并对酶学性质进行研究。结果表明：阿魏侧耳产漆酶的最佳培养时间为9 天，最佳碳源为可溶性淀粉，最佳氮源为蛋白胨，培养基最适的初始pH 6.0，0.1 mmol/L愈创木酚和1 mmol/L Mn2 对阿魏侧耳产漆酶有明显的促进作用，使酶活分别提高了32.2%和30.8%。对阿魏侧耳产的漆酶进行酶学性质研究，其最适反应温度为45℃，在40、45℃下酶活相对稳定，48 h 后能保持较高酶活。最适反应pH 6.0，在pH 4.0~7.0 时，漆酶均有较高活性，在pH 5.0~7.0时有较高稳定性，48 h 后相对酶活保持在70%以上。阿魏侧耳在发酵条件优化后具有较高的产漆酶能力，所产漆酶的最适温度相对较高，最适pH为中性偏酸性。

关键词: 草地早熟禾, 草地早熟禾, 立枯丝核菌侵染, 生理响应

Abstract: The study aims to optimize fermentation conditions such as carbon source, nitrogen source, temperature, pH value, inducer and metal iron for laccase production by Pleuratus ferulae, and investigate its enzymatic properties, in order to earn enzyme solution of high activity for pesticides degradation. The results showed that the optimal time of incubation was 9 days, the optimal carbon source and nitrogen source was soluble starch and peptone, the initial optimal pH of culture medium was 6.0, 0.1 mmol/L guaiacol and 1 mmol/L Mn2 had a great effect on the production of laccase. The study for the enzymatic property of Pleuratus ferulae showed that the optimum temperature was 45℃ and relatively stable at 40 and 45℃ with a high activity after 48 hours. The optimum pH value for the laccase was 6.0，the high laccase activity also could be obtained at the pH value range of 4.0-7.0 and kept stable at the pH value from 5.0 to 7.0, the laccase activity remained 70% after 48 hours. Pleuratus ferulae had high capacity of laccase production after the optimization of fermentation conditions, its optimum temperature was a little high relatively and the optimum pH value was natural and weak-acid.