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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (20): 50-54.doi: 10.11924/j.issn.1000-6850.casb16070095

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

“福春1号”大白菜一代杂种纯度的SRAP鉴定

钟开勤,曾小玲,朱朝辉,陈文辉,赵瑞丽   

  1. 福州市蔬菜科学研究所,福州市蔬菜科学研究所,福州市蔬菜科学研究所,福州市蔬菜科学研究所,福州市蔬菜科学研究所
  • 收稿日期:2016-07-18 修回日期:2016-09-18 接受日期:2016-09-21 出版日期:2017-07-18 发布日期:2017-07-18
  • 通讯作者: 钟开勤
  • 基金资助:
    福建省福州市科技项目“晚抽薹春大白菜种质资源收集鉴定研究与利用”(2014-N-103)。

Purity Identification of Chinese Cabbage Hybrid‘Fuchun No.1’by SRAP Markers

  • Received:2016-07-18 Revised:2016-09-18 Accepted:2016-09-21 Online:2017-07-18 Published:2017-07-18

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摘要： ‘福春1 号’大白菜是福州市蔬菜科学研究所选育的晚抽薹春白菜新品种。为鉴定该品种的纯度，利用80 对SRAP引物组合对‘福春1 号’及其亲本混合池DNA模板进行扩增，筛选出杂交种具有亲本互补特征带的引物组合，并利用该组合对田间栽培的‘福春1 号’大白菜幼苗进行纯度检测。本研究最终筛选出引物组合M2 E4，其对杂交种扩增产生亲本互补的特征带，可将亲本和杂交种有效区分。利用这对SRAP引物组合对102 株‘福春1 号’大白菜幼苗进行纯度检测，检测纯度为94.12%。SRAP标记鉴定结果与田间调查结果基本一致。SRAP分子标记技术可用于‘福春1号’大白菜的纯度鉴定。

关键词: EOS/MODIS, EOS/MODIS, 蝗灾, NDVI, 相对变化量

Abstract: ‘Fuchun No.1’is a new late bolting spring Chinese cabbage cultivar breeded by Fuzhou Institute of Vegetable Science. In order to identify the purity of‘Fuchun No.1’, 80 pairs of SRAP primers were used to amplify the DNA templates of‘Fuchun No.1’and its parental pooling. The pairs of primers which contained bands of parental complementary characteristics were screened out and used to identify the purity of‘Fuchun No.1’seedlings cultivated in field. In the study, the pairs of primer M2＋E4 which contained bands of parental complementary characteristics were screened out and used to distinguish parents and hybrids effectively. The pairs of SRAP primer were adopted to identify the purity of 102‘Fuchun No.1’seedlings and the seed purity was 94.12% . The identification of SRAP markers is consistent with field survey results. SRAP molecular markers can be used to identify the purity of‘Fuchun No.1’.