不同品种羊miR-1298-5p及其潜在靶基因TGF-βR1表达的比较研究

doi:10.11924/j.issn.1000-6850.casb16080015

中国农学通报 ›› 2017, Vol. 33 ›› Issue (27): 49-54.doi: 10.11924/j.issn.1000-6850.casb16080015

所属专题：生物技术；畜牧兽医

不同品种羊miR-1298-5p及其潜在靶基因TGF-βR1表达的比较研究

张桂山,徐晶,孙丽敏,白曼,项露杰,姜怀志

吉林农业大学动物科学技术学院,吉林农业大学动物科学技术学院,吉林农业大学动物科学技术学院,吉林农业大学动物科学技术学院,吉林农业大学动物科学技术学院,吉林农业大学动物科学技术学院

收稿日期:2016-08-02 修回日期:2016-09-17 接受日期:2016-09-20 出版日期:2017-10-09 发布日期:2017-10-09
通讯作者: 姜怀志
基金资助:
吉林省科技发展计划项目“肉毛兼用型细毛羊新品种选育及高效扩繁技术”(20160204018NY)。

Comparative Study on Expression of miR-1298-5p and its Potential Target Gene TGF-βR1 in Fine-wool Sheep and Cashmere Goats

Received:2016-08-02 Revised:2016-09-17 Accepted:2016-09-20 Online:2017-10-09 Published:2017-10-09

摘要/Abstract

摘要： 旨在探讨miR-1298-5p 与TGF-βR1 在不同品种羊皮肤毛囊不同发育时期的潜在关系，为研究miR-1298-5p 与TGF-βR1 对皮肤毛囊发育作用提供理论研究数据。以绒山羊和细毛羊皮肤毛囊组织为材料，通过生物信息学方法预测miR-1298-5p 的靶基因，采用qRT-PCR对miR-1298-5p 与其潜在靶基因TGF-βR1 进行核酸水平表达检测，利用Western blot 对潜在靶基因TGF-βR1 进行蛋白水平表达检测。生物信息学预测结果表明TGF-βR1 的3''UTR 存在miR-1298-5p 种子区结合位点。miR-1298-5p 在绒山羊和细毛羊皮肤毛囊发育的生长期到退行期相对表达量上调而退行期到休止期相对表达量下调，miR-1298-5p 在不同品种羊皮肤毛囊不同发育时期呈现差异性表达。绒山羊皮肤毛囊发育生长期TGF-βR1基因mRNA的相对表达量高于细毛羊，而退行期和休止期低于细毛羊；从表达趋势上看，TGF-βR1 与miR-1298-5p 表达趋势相反，呈现负调控趋势，说明TGF-βR1 可能为miR-1298-5p 的靶基因。TGF-βR1蛋白在绒山羊皮肤毛囊发育生长期的相对表达量高于细毛羊，而退行期和休止期则低于细毛羊，与核酸水平相对表达量趋势相同，但都与miR-1298-5p 表达趋势相反，更进一步说明TGF-βR1 可能为miR-1298-5p的靶基因。

关键词: 古水稻土, 古水稻土, 铁形态, 全铁, 剖面分布

Abstract: The study aimed to investigate the regulation of miR-1298-5p and its potential target gene TGF-βR1 on skin and hair follicles development at different stages in fine-wool sheep and cashmere goats for obtaining theoretical research data on miR-1298-5p and TGF-βR1 in the development of skin and hair follicles. Using skin and hair follicles tissues from cashmere goats and fine-wool sheep at different stages as materials, potential target genes of miR-1298-5p were predicted by bioinformatics analysis. Expression of miR-1298-5p and its potential target gene TGF-βR1 were determined by qRT-PCR. Expression of TGF-βR1 protein was determined by Weston blot. Bioinformatics analysis showed that 3''UTR of TGF-βR1 from fine-wool sheep and cashmere goats presented a binding site of miR-1298-5p. Relative expression of miR-1298-5p from fine-wool sheep and cashmere goats were both up regulated from anagen to catagen and down regulated from catagen to telogen, and presented difference between fine-wool sheep and cashmere goats. Relative expression of TGF-βR1 mRNA in cashmere goats was higher than that in fine-wool sheep at anagen and lower at catagen and telogen. And miR-1298-5p and TGF-βR1 showed a negative regulation trend, which implied that TGF-βR1 might be the target gene of miR-1298-5p. Relative expression of TGF-βR1 protein in cashmere goats was also higher than that in fine-wool sheep at anagen and lower at catagen and telogen, being the same as the relative expression of TGF-βR1 mRNA, which further implied that TGF-βR1 was target gene of miR-1298-5p.

张桂山,徐晶,孙丽敏,白曼,项露杰,姜怀志. 不同品种羊miR-1298-5p及其潜在靶基因TGF-βR1表达的比较研究[J]. 中国农学通报, 2017, 33(27): 49-54.

参考文献

[1] MA J B,HUANG Y. Post-transcriptional regulation of miRNA biogenesis and functional[J]. Front Biol, 2010, 5(1):32-40.
[2]　Chekulaeva M, Filipowiz W. Mechanisms of miRNA mediated Post-transcriptional regulation animal　cells[J]. Curr Opin Cell Biol, 2009, 21(3):452-460.
[3] 贾小云,金雷皓,苗潋涓,等. miR-663通过靶向TGF-β1调控羊驼黑色素细胞的黑色素生成[J]. 中国农业科学,2015,48(1)：165-173.
[4] Liu G, Liu R, Li Q, et al. Identification of microRNAs in wool follicles during anagen, catagen, and telogen phases in Tibetan sheep[J]. Plos One, 2013, 8(10): 377-380.
[5]唐晓惠,柳广斌,杜小勇等. miR-184及miR-205在绵羊不同生长时期毛囊及其他组织中的表达研究[J]. 畜牧兽医学报,2012(12),1897-1903.
[6]樊凯军,尹俊. 绒山羊70d 胚胎皮肤小分子ＲNA 文库构建及miＲNA 鉴定[J]. 生物技术,2014,24(1),32-36._[7] _朱芷葳_,_贺俊平_,_程志学_._{ｍｉｃｒｏＲＮＡｓ}_{在成年羊驼皮肤组织中的表达}_[J]. _{畜牧兽医学报}_{,2010,41(10):1342-1345.}
[8] Sood P, Krek A, Zavolan M, et al. Cell-type-specific signatures of microRNAs on target mRNA expression[J]. Proc Natl Acad Sci USA, 2006, 103(8): 2746-2751.
[9] Derynck R, Zhang Y E. Smad-dependent and Smad-independent pathways in TGF-beta family signaling. Nature, 2003, 425(6958):577-584.
[10] 曾东,余文林,杨传红,等. TGF-β各亚型与其受体在猪皮肤及毛囊中的表达[J]. 中国美容医学,2009,18(5)：641-643.
[11] Gallo E M, Loch D C, Habashi J P, et al. Angiotensin II–dependent TGF-β signaling contributes to Loeys-Dietz syndrome vascμLar pathogenesis[J]. The journal of Clinical Investigation. 2014,124(1): 448-460.
[12]Feng X H, Derynck R. Specificity and versatility in TGF-beta signaling through Smads[J]. Annual Review of Cell and Developmental Biology, 2005, 21: 659-693.
[13]Park S H. Fine tuning and cross-talking of TGF-beta signal by inhibitory smads[J]. Journal of Biochemistry and Molecular Biology,2005, 38(1): 9-16.
[14] Lebrin F, Deckers M, Bertolino P, et al. TGF-β receptor function in the endothelium[J]. Cardiovasc Res, 2005, 65(3): 599-608.
[15] James J M, Nalbandian A, Mukouyama Y S. TGFβ signaling is required for sprouting lymphangiogenesis during lymphatic network development in the skin[J]. Development. 2013,140(18): 3903-3914.
[16] Zimmerman C M, Padgett R W. Transforming growth factor beta signaling mediators and modulators[J] .Gene, 2000,249 (16) :17-30.
[17] Rojas A, Padidam M, Cress D, et al. TGFβ receptor levels regμLate the specifility of signaling pathway activation and biological effects of TGFβ[J]. Biophys Acta,2009,1793(7):1165-1173.
[18] Mazzieri R, Jurukovski V, Obata H, et al. Expression of truncated latent TGF-beta- binding protein modulates TGF-beta signaling[J].Cell Sci,2005,l18(l0)：2177-2187.
[19] 曲海娥. MIR-125b在细毛羊与绒山羊皮肤组织中差异表达及其靶基因的筛选与鉴定[D].长春:吉林大学,2014.
[20] Yuan C, Wang XL, Geng RQ, et al. Discovery of cashmere goat (Capra hircus) microRNAs in skin and hair follicles by Solexa sequencing[J]. BMC Genomics, 2013, 14(1): 1-10.

不同品种羊miR-1298-5p及其潜在靶基因TGF-βR1表达的比较研究

Comparative Study on Expression of miR-1298-5p and its Potential Target Gene TGF-βR1 in Fine-wool Sheep and Cashmere Goats

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