绒毛白蜡smh1基因编码区的克隆及序列分析

doi:10.11924/j.issn.1000-6850.casb16120077

中国农学通报 ›› 2017, Vol. 33 ›› Issue (15): 49-55.doi: 10.11924/j.issn.1000-6850.casb16120077

所属专题：生物技术

绒毛白蜡smh1基因编码区的克隆及序列分析

张冬杰¹,燕丽萍²,吴其超¹,刘翠兰²,王因花²,吴德军²

(¹山东农业大学林学院,山东泰安 271000；²山东省林业科学研究院/山东省林木遗传改良重点实验室,济南 250000）

收稿日期:2016-12-15 修回日期:2017-05-05 接受日期:2017-02-24 出版日期:2017-05-26 发布日期:2017-05-26
通讯作者: 吴德军
基金资助:
国家“十二五”科技支撑计划专题“白蜡种质资源发掘与创新利用”(2013BAD01B06-6)；山东省科技发展计划项目“白蜡种质资源分子评价与体胚发育技术研究”(2014GNC111006)。

Cloning and Sequence Analysis of Fraxinus velutina smh1 Gene Coding Region

Zhang Dongjie¹, Yan Liping², Wu Qichao¹, Liu Cuilan², Wang Yinhua², Wu Dejun²

(¹College of Forestry, Shandong Agricultural University, Taian Shandong 271000; ²Shandong Academy of Forestry/Shandong Key Laboratory of Forest Tree Genetic Improvement, Jinan 250000)

Received:2016-12-15 Revised:2017-05-05 Accepted:2017-02-24 Online:2017-05-26 Published:2017-05-26

摘要/Abstract

摘要： ：为探究绒毛白蜡smh1 端粒结合蛋白基因在端粒中的作用以及在植物生长发育过程中的调控机制，进行绒毛白蜡smh1 端粒结合蛋白基因克隆及分析。以绒毛白蜡幼苗为试材，Illumina HiSeq 2000 高通量测序技术获得的绒毛白蜡转录组Unigene 数据为基础，采用RT-PCR方法克隆绒毛白蜡smh1 基因编码区序列并进行生物信息学分析。结果表明：从绒毛白蜡叶片中成功克隆出一个smh 类端粒结合蛋白基因编码区序列。基因编码区长度为879 bp，可编码292 个氨基酸，分子量为31.66 kDa，等电点为6.5，原子总数为4457 个，分子式为C1371H2242N3 92O439S13。Conserved Domains 数据库对蛋白质的保守结构域进行分析，结果显示该蛋白序列具有植物端粒结合蛋白Smh (single myb histone)亚家族典型的结构特征，包括位于N-端的Myb样结构域以及中间的组蛋白H1/5 样结构域。将FvSmh1 端粒结合蛋白与含有telobox 基序的其他端粒结合蛋白进行系统进化树的构建，结果显示SMH 端粒结合蛋白与TRF1 和TRF2 同属一个分支，具有较近的关系。从上述分析结果可以推断所克隆的绒毛白蜡smh1 基因可能对植物端粒结构的维持和功能的发挥具有重要作用。

关键词: 近红外光谱, 近红外光谱, 糖碱比, 氮碱比, 施木克值

Abstract: To study the function of Fraxinus velutina smh1 telomere binding protein gene in telomere and regulation mechanismin in the process of plant growth and development, we clone and analysis Fraxinus velutina smh1 telomere binding protein gene. By selecting Fraxinus velutina seedling as experiment material and based on the transcriptome Unigene data which gained by Illumina HiSeq 2000 high throughput sequencing technology, this experiment choose RT-PCR method to clone Fraxinus velutina smh1 Gene Coding Region and to make bioinformatics analysis. The results showed that a sequence of Fraxinus velutina smh gene coding region was cloned successfully. Gene coding region length is 879bp, encoding 292 amino acids with a mass of 31.66 kDa, pI of 6.5, atoms to the total number4457, molecular formula C1371H2242N392O439S13. Conserved Domains database analyzed the protein conserved domains and the result shows that this protein sequence have the typical structures of plant Smh telomere binding protein subfamily including the Myb like domain located at the N-terminal and the middle histone H1/5 like domain. Carrying on constructing phylogenetic trees between FvSmh1 and other telomere binding protein which contain telobox shows that both SMH telomere binding protein and TRF1, TRF2 belong to a same branch and have close relationship with each other. From the analysis above, it is inferred that the cloned Fraxinus velutina smh1 gene may play an important role in maintaining the structure and function of the plant telomere.

中图分类号:

S722.5

张冬杰,燕丽萍,吴其超,刘翠兰,王因花,吴德军. 绒毛白蜡smh1基因编码区的克隆及序列分析[J]. 中国农学通报, 2017, 33(15): 49-55.

Zhang Dongjie,Yan Liping,Wu Qichao,Liu Cuilan,Wang Yinhua and Wu Dejun. Cloning and Sequence Analysis of Fraxinus velutina smh1 Gene Coding Region[J]. Chinese Agricultural Science Bulletin, 2017, 33(15): 49-55.

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绒毛白蜡smh1基因编码区的克隆及序列分析

Cloning and Sequence Analysis of Fraxinus velutina smh1 Gene Coding Region

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