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中国农学通报 ›› 2018, Vol. 34 ›› Issue (10): 131-137.doi: 10.11924/j.issn.1000-6850.casb17030160

所属专题: 烟草种植与生产

• 食品 营养 检测 安全 • 上一篇    下一篇

UPLC法同时测定烤烟中的新绿原酸、绿原酸、隐绿原酸、莨菪亭、芸香苷和莰菲醇基-3-芸香糖苷

李 力,李东亮,邓发达,罗 诚,沈 怡,薛 芳   

  1. 四川中烟工业有限责任公司,四川中烟工业有限责任公司,四川中烟工业有限责任公司,四川中烟工业有限责任公司,四川中烟工业有限责任公司,四川中烟工业有限责任公司
  • 收稿日期:2017-03-24 修回日期:2017-05-02 接受日期:2017-05-25 出版日期:2018-04-10 发布日期:2018-04-10
  • 通讯作者: 罗 诚
  • 基金资助:
    四川中烟工业有限责任公司科研项目“打叶复烤与醇化对烟叶品质影响研究”(川渝烟工技研[2013]164 号:1-08)。

Analysis of 6 Polyphenols in Flue-cured Tobacco by UPLC

  • Received:2017-03-24 Revised:2017-05-02 Accepted:2017-05-25 Online:2018-04-10 Published:2018-04-10

摘要: [目的]为了快速、准确地测定烟草中绿原酸、芸香苷和莨菪亭等多酚类化合物的含量,[方法]建立了一种同时测定了烤烟中的新绿原酸、绿原酸、隐绿原酸、莨菪亭、芸香苷和莰菲醇基-3-芸香糖苷的超高效液相色谱(UPLC)分析方法。方法采用HSS T3高强度硅胶色谱柱(HSS: High Strength Silica),梯度洗脱。[结果]实验方法的加标回收率在94.4 ~ 106.8%之间,检出限为3.7 ~ 9.9 μg/g,相对标准偏差0.9 ~ 3.4%(n=5),标准溶液的相关系数均大于0.9995。采用实验方法测定了不同来源的18个烤烟样品,结果发现烤烟中6种多酚的含量从高到低依次为绿原酸>芸香苷>隐绿酸>新绿原酸>莰菲醇基-3-芸香糖苷>莨菪亭。[结论]与传统的高效液相色谱法(HPLC)相比,实验方法简单,分析时间短,能够满足对烤烟中多酚含量的测定要求。

关键词: 墨吉明对虾, 墨吉明对虾, 副溶血弧菌, 人工感染

Abstract: To determine chlorogenic acid, rutin, scopletin and other polyphenols in tobacco accurately, an ultra performance liquid chromatography (UPLC) method for the determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, scopletin, rutin, and kaempferol- 3-O-rutinoside in flue-cured tobacco was developed by using a HSS T3 column with gradient elution. The precision of measurement for this method ranged from 0.9% to 3.4% (n=5) in terms of relative standard deviation. The recoveries of standard addition and limits of detection were in the range of 94.4%-106.8% and 3.7-9.9 μg/g, respectively. The linear correlative coefficients for the 6 polyphenols were better than 0.9995. The content of polyphenols in 18 flue-cured tobacco leaves from different cultivars and production areas was determined by this method. The results showed that the content distribution of the 6 polyphenols was chlorogenic acid> rutin> cryptochlorogenic acid> neochlorogenic acid> kaempferol- 3- O- rutinoside> scopletin. Compared with high performance liquid chromatography (HPLC), the new method is rapid, simple, and meets the requirements of determination for polyphenols in flue-cured tobacco leaves.

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