GlyCAM-1在自然发情绵羊子宫中的表达研究

doi:10.11924/j.issn.1000-6850.casb17030162

中国农学通报 ›› 2018, Vol. 34 ›› Issue (11): 123-127.doi: 10.11924/j.issn.1000-6850.casb17030162

所属专题：畜牧兽医

GlyCAM-1在自然发情绵羊子宫中的表达研究

朱伯阳,刘迪,白若雨,谷传慧,杨健,詹同彤,王相国,齐晓龙,盛熙辉,梁鸿斌,郭勇,倪和民

北京农学院动物科学技术学院,北京农学院,北京农学院,北京市动物疫病预防控制中心,北京农学院,北京农学院,北京农学院,北京农学院,北京农学院,北京奶牛中心,北京农学院,北京农学院

收稿日期:2017-03-24 修回日期:2017-04-20 接受日期:2017-05-02 出版日期:2018-04-16 发布日期:2018-04-16
通讯作者: 倪和民
基金资助:
2015 年度国家自然基金项目“MUC-1 协调GlyCAM-1 调控绵羊子宫容受态的研究”(31470118)；2013 年度北京市教委北京市属高等学校创新团队建设与教师职业发展计划项目“体细胞转基因克隆肉牛新品系培育与利用”(PXM2013_014207 000067)。

Expression of GlyCAM-1 in Uteri of Sheep in Oestrous Cycle

朱伯阳,,,,,,,,,, and

Received:2017-03-24 Revised:2017-04-20 Accepted:2017-05-02 Online:2018-04-16 Published:2018-04-16

摘要/Abstract

摘要： 旨在研究GlyCAM-1 在绵羊自然发情子宫组织中的表达变化，以期为周期发情绵羊子宫中GlyCAM-1 的表达受孕酮诱导调控的推测提供支持。运用实时荧光定量PCR和免疫印迹法对绵羊自然发情后子宫第10、12、14、16 和18 天GlyCAM-1 mRNA和蛋白表达变化进行检测。实时荧光定量PCR结果显示：在绵羊发情后的第10~18 天之间，子宫组织中GlyCAM-1 的表达呈现先下降再上升的趋势，发情后的第10 天GlyCAM-1 在转录水平表达出现峰值，并且显著的高于另外4 个时段(P<0.05)，第12 天GlyCAM-1 mRNA水平显著下降(P<0.05)，第14~16 天的表达量保持相对恒定，第18 天GlyCAM-1 mRNA水平再次显著升高(P<0.05)，但仍低于第10 天水平(P<0.05)。Western Blotting 检测结果显示：绵羊在自然发情后的第10~18 天，子宫内膜上的GlyCAM-1 蛋白表达量的变化亦呈现出先下降后上升的趋势，与其他4 个时间点相比第10 天的蛋白表达量达到峰值(P<0.05)，第12~16 天GlyCAM-1 的蛋白表达量没有显著变化(P>0.05)，GlyCAM-1 的蛋白表达量在第18 天显著升高(P<0.05)。GlyCAM-1 在转录与翻译水平的表达变化一致，呈现先下降后上升的趋势。该结果为周期发情绵羊子宫中GlyCAM-1 的表达可能受孕酮诱导调控的推测提供支持。

关键词: 玉米, 玉米, 杂交种, 遗传多样性, 聚类分析

Abstract: To study the expression of GlyCAM-1 in the uterus of natural oestrus sheep. In order to provide support for the regulation of progesterone induced GlyCAM-1 expression in ovine uterus during estrous cycle. [Methods] The mRNA and protein expressions of GlyCAM-1 in 10th, 12th, 14th, and 18th days after the natural estrus in sheep were detected by real-time fluorescent quantitative PCR and Western blotting. [Results] Real time fluorescence quantitative PCR results showed that: between the sheep estrous after 10th to 18th days, the expression of GlyCAM-1 in uterine tissue showed a rise and then decline.On the 10th days after estrus,The GlyCAM-1 expression peak at the transcriptional level showed up, and significantly higher than the other 4 periods (P<0.05), the expression of 12th days GlyCAM-1 mRNA decreased significantly (P<0.05), the expression amount of 14th to 16th days remained relatively constant, the expression of GlyCAM-1 in 18th days mRNA again increased significantly (P<0.05), but the relative expression amount is still less than 10th days expression (P<0.05). Western Blotting test results show that: in the 10th to 18th days after estrus GlyCAM-1 protein expression in sheep endometrium was also showed first decreased and then increased, compared with the other 4 time points for 10th days the amount of rotein expression reached the peak (P<0.05), the GlyCAM-1 protein expression on 12th, 14th, 16 th days showed no significant change (P > 0.05), the expression of GlyCAM-1 protein was significantly higher in 18th days (P<0.05). The expression level of GlyCAM-1 is consistent with the level of transcription and translation. [Conclusion] the transcription and translation expression of GlyCAM-1 in the uterus tissue of spontaneous estrus sheep showed a downward trend after the embryo implantation. It is suggested that the expression of GlyCAM-1 in the uterus of estrous cycle may be supported by the regulation of progesterone induction.

朱伯阳,刘迪,白若雨,谷传慧,杨健,詹同彤,王相国,齐晓龙,盛熙辉,梁鸿斌,郭勇,倪和民. GlyCAM-1在自然发情绵羊子宫中的表达研究[J]. 中国农学通报, 2018, 34(11): 123-127.

朱伯阳,,,,,,,,,, and . Expression of GlyCAM-1 in Uteri of Sheep in Oestrous Cycle[J]. Chinese Agricultural Science Bulletin, 2018, 34(11): 123-127.

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GlyCAM-1在自然发情绵羊子宫中的表达研究

Expression of GlyCAM-1 in Uteri of Sheep in Oestrous Cycle

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