TMV侵染烟草的早期响应蛋白分析

doi:10.11924/j.issn.1000-6850.casb17090051

中国农学通报 ›› 2018, Vol. 34 ›› Issue (9): 40-47.doi: 10.11924/j.issn.1000-6850.casb17090051

所属专题：生物技术；烟草种植与生产

TMV侵染烟草的早期响应蛋白分析

郭尧,盛阳阳,谢腾腾

郑州外国语新枫杨学校,河南农业大学生命科学学院,河南农业大学生命科学学院

收稿日期:2017-09-10 修回日期:2018-03-13 接受日期:2017-12-22 出版日期:2018-03-28 发布日期:2018-03-28
通讯作者: 郭红祥
基金资助:
河南省烟草公司科技项目“烟草花叶病分子监测与预警中标志基因和蛋白质的筛选”(142106000187)；河南省驻马店市公司科技项目“烟草土壤改良剂在驻马店烟田的研究与应用”(201741170020121)。

Early Responsive Proteins in Tobacco Infected by Tobacco Mosaic Virus

郭尧, and

Received:2017-09-10 Revised:2018-03-13 Accepted:2017-12-22 Online:2018-03-28 Published:2018-03-28

摘要/Abstract

摘要： 为了完善烟草花叶病的预警防控体系,分析烟草感染烟草普通花叶病毒后的蛋白质组早期变化,筛选烟草的早期响应蛋白。[方法]采用iTRAQ技术分析了蛋白质组变化,采用qPCR技术检测基因的表达。[结果]蛋白质组分析发现160个差异表达蛋白质,包括有64个上调表达蛋白和96个下调表达蛋白,这些差异表达蛋白质主要与氧化还原平衡调节、RNA降解、叶绿体组成及光合作用、逆境胁迫响应有关；qPCR结果显示这些差异蛋白质的mRNA表达变化趋势与iTRAQ数据基本一致；及时施用抗病毒药剂(氨基寡糖素和吗胍.乙酸铜)和植物诱抗剂(盐酸吗啉胍和氮苷吗啉胍)能够预防和控制烟草花叶病的发生,但同时发现PR10、LHCB、POD、HSP90、14-3-3和AGO1等蛋白的表达也受到了影响。[结论]试验筛选出一些TMV感染相关的烟草早期响应蛋白,不仅有助于阐述烟草花叶病发生的机制,也可用于筛选新的抗病毒药剂和植物诱抗剂。

关键词: 蔬菜, 蔬菜, 重金属污染, 太原市

Abstract: In order to improve the early warning and prevention system of tobacco mosaic disease, the early responsive proteins in tobacco infected by TMV were screened through detecting the proteome changes. [Methods] iTRAQ was used to analyze the proteome changes and the genes expression was detected with qPCR method. [Results] Proteomic analysis showed that there were 160 differentially expressed proteins, including 64 up-regulated and 96 down-regulated proteins. The functions of these differential proteins were related to redox balance, RNA decay, stress response, chloroplast structure and photosynthesis. qPCR results demonstrated that the change trend in mRNA expressions of these differential proteins was consistent with iTRAQ data. Tobacco mosaic disease could be effectively controlled by timely application of antiviral agents and plant resistance inducers, which induced the changes in expression of PR10, LHCB, POD, HSP90, 14-3-3 and AGO1. [Conclusion] Some early responsive proteins were identified in tobacco infected by TMV, which will be helpful for clarifying the pathogenesis mechanism of tobacco mosaic disease and screening new antiviral agents and plant resistance inducers.

中图分类号:

S435.72

郭尧,盛阳阳,谢腾腾. TMV侵染烟草的早期响应蛋白分析[J]. 中国农学通报, 2018, 34(9): 40-47.

郭尧, and . Early Responsive Proteins in Tobacco Infected by Tobacco Mosaic Virus[J]. Chinese Agricultural Science Bulletin, 2018, 34(9): 40-47.

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TMV侵染烟草的早期响应蛋白分析

Early Responsive Proteins in Tobacco Infected by Tobacco Mosaic Virus

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