橡胶籽油替代豆油对丝尾鳠幼鱼生长性能、消化酶活性和抗氧化功能的影响

doi:10.11924/j.issn.1000-6850.casb18010119

摘要/Abstract

摘要： 旨在研究饲料中橡胶籽油替代豆油对丝尾鳠幼鱼生长性能、消化酶活性和抗氧化功能的影响，并确定其适宜替代比例。以橡胶籽油分别替代0%(R0)、25%(R25)、50%(R50)、75%(R75)和100%(R100)豆油，配制5种等氮等能饲料，进行8周饲养试验。试验表明：（1）R25替代组体增重和日增重系数均显著高于其他处理组(P?0.05)；（2）R25、R50和R75替代组肠道脂肪酶活性显著高于R100替代组；R25替代组肝脏胰蛋白酶活性显著高于其他处理组；类似地，R25替代组肝脏脂肪酶活性显著高于R75和R100替代组；（3）随着橡胶籽油替代豆油比例的提高，肝脏ALT、AST和GGT活性呈先上升后下降趋势，其中以R25替代组最高；R100替代组肝脏GDH活性显著低于R0组；（4）R100替代组血浆POD活性显著低于R50和R75替代组；类似地，R100替代组血浆T-AOC活性显著低于R25和R75替代组；R100替代组肝脏MDA含量显著高于其他处理组。结果表明，橡胶籽油替代25%~75%豆油对丝尾鳠饲料利用率和抗氧化功能均无明显负面影响，且替代25%明显提高丝尾鳠生长性能；但完全替代明显抑制丝尾鳠抗氧化功能。

关键词: 发芽糙米, 发芽糙米, 有色米, 游离氨基酸

Abstract: This study aims at investigating the effects of replacing soybean oil with rubber seed oil on growth performance, digestive enzyme activity and antioxidant capacity of Asian red- tailed catfish (Hemibagrus wyckioides), and determining the appropriate replacement rate of soybean oil by rubber seed oil. Five isoproteic and isolipidic experimental diets (R0, R25, R50, R75 and R100) were formulated with rubber seed oil replacing 0%, 25%, 50%, 75% and 100% soybean oil in diets, respectively. The feeding trial lasted for 8 weeks. The results showed that: (1) the weight gain and daily gain coefficient of fish fed R25 diet were significantly higher than that of the other diets (P<0.05); (2) the intestinal lipase activity was higher in R25, R50 and R75 diets, compared to R100 diet; the intestinal trypsin activity of R25 diet was significantly higher than that of the other diets; similarly, the hepatic lipase activity was higher in R25 diet compared with fish fed R75 and R100 diets; (3) with the increasing of rubber seed oil replacement level, the hepatic alanine transaminase, aspartate transaminase and glutamyl transpeptidase activities firstly increased and then decreased, the highest values were observed in R25 diet; the hepatic glutamate dehydrogenase activity of R100 diet was significantly lower than that of R0 diet; (4) the plasma peroxidase of R100 diet was significantly lower than that in R25 and R75 diets; similarly, the plasma total antioxidant capacity of R100 diet was significantly lower than that of R25 and R75 diets; the hepatic malondialdehyde content was significantly higher in R100 diet compared with fish fed the other diets. These results indicate that replacing 25%-75% soybean oil with rubber seed oil has no obvious negative effect on feed utilization and antioxidant capacity, and replacing 25% soybean oil is beneficial to improve the growth performance, but total replacement markedly depresses the antioxidant capacity of Hemibagrus wyckioides.

张新党,林贝贝,施洪跃,余俊,邓君明. 橡胶籽油替代豆油对丝尾鳠幼鱼生长性能、消化酶活性和抗氧化功能的影响[J]. 中国农学通报, 2019, 35(11): 146-154.

参考文献

[1]胡水鑫,王骥腾,韩涛,等.水产饲料脂肪来源及前景分析[J].安徽农学通报,2012,18(9):183-185.
[2]艾庆辉,严晶,麦康森.鱼类脂肪与脂肪酸的转运及调控研究进展[J].水生生物学报,2016,40(4):859-868.
[3]顾夕章,高启平,谢骏,等.饲料中亚麻籽替代部分大豆油对异育银鲫鱼苗生长性能、肌肉组成及血清生化指标的影响[J].动物营养学报,2012, 24(11):2272-2278.
[4]肖桂秀.橡胶树地域性优良品种选育[C].全国林木遗传育种大会.2008.
[5]童长征.天然橡胶期货市场分析[J].橡胶科技,2017,15(2):5-10.
[6]彭艳,范武波,孙娟.关于橡胶籽综合利用情况的研究报告[J].中国热带农业,2012(4):6-7.
[7]杨晓琴,郑志锋,郑云武,等.橡胶籽油的分析表征[J].食品工业科技,2016,37(24):128-131.
[8]王坤,陶琳丽,詹瑰然,等.橡胶籽油替代豆油对虹鳟幼鱼生长性能、血液及肝脏生化指标的影响[J].云南农业大学学报:自然科学,2017,32(4):627-636.
[9]刘明典,陈大庆,段辛斌,等.澜沧江云南段鱼类区系组成与分布[J].中国水产科学,2011,18(1):156-170.
[10]Ziv G, Baran E, Nam S, et al. Trading-off fish biodiversity, food security, and hydropower in the Mekong River Basin[J]. Proceedings of the National Academy of Sciences of the United States of America,2012,109(15):5609-5614.
[11]Hee N H, Rainboth W J. The bagrid catfish genus Hemibagrus (Teleostei: Siluriformes) in central Indochina with a new species from the Mekong River[J]. Raffles Bulletin of Zoology,1999,47(2):555-576.
[12]薛晨江,李晓双.丝尾鳠苗种培育技术[J].科学养鱼,2012(4):7-7.
[13]阴双雨.澜沧江丝尾鳠生物学和资源[D].华中农业大学,2011.
[14]陈云芬.土著丝尾鳠人工繁育获成功.云南日报,2010,4(6): 2.
[15]Deng J, Mai K, Chen L, et al. Effects of replacing soybean meal with rubber seed meal on growth, antioxidant capacity, non-specific immune response, and resistance to Aeromonas hydrophila, in tilapia (Oreochromis niloticus × O.aureus)[J]. Fish Shellfish Immunology,2015,44(2): 436-444.
[16]师梅梅.荔枝草提取物的体外抗氧化活性研究[D].陕西师范大学,2008.
[17]姜召山,王锐,刘辉宇.亚麻酸对鲫鱼生长性能和鱼体成分的影响[J].粮食与饲料工业,2010(6):56-58.
[18]关勇.亚麻籽油对吉富罗非鱼生长、体组成、脂质代谢及抗氧化能力的影响[D].西南大学,2014.
[19]Wu F C, Chen H Y. Effects of dietary linolenic acid to linoleic acid ratio on growth, tissue fatty acid profile and immune response of the juvenile grouper Epinephelus malabaricus[J]. Aquaculture,2012,s324-325(1):111-117.
[20]Gersande B, Judithg M, Patrick K. Influence of different dietary 18:3n-3/18:2n-6 ratio on growth performance, fatty acid composition and hepatic ultrastructure in Eurasian perch, Perca fluviatilis[J]. Aquaculture,2008,284(1):144-150.
[21]Gangdhara B N,Eesha M C,Varghese T J,et al. Effect of varying protein and lipid levels on the growth of Rohu, Labeo rohita[J]. Asian Fisheries Society,1997,10(2):139-147.
[22]Caballero M J, Obach A, Rosenlund G, et al. Impact of different dietary lipid sources on growth, lipid digestibility, tissue fatty acid composition and histology of rainbow trout, Oncorhynchus mykiss[J]. Aquaculture,2002,214(1-4):253-271.
[23]Jutfelt F, Olsen R E, Bj?rnsson B T, et al. Parr-smolt transformation and dietary vegetable lipids affect intestinal nutrient uptake, barrier function and plasma cortisol levels in Atlantic salmon[J]. Aquaculture,2007,273(2-3):298-311.
[24]Ferreira M W, Dearaujo F G, Costa D V, et al. Influence of dietary oil sources on muscle composition and plasma lipoprotein concentration in Nile tilapia, Oreochromis niloticus[J]. Journal of the World Aquaculture Society,2011,42(1):24.
[25]Fernandez M L, West K L. Mechanisms by which dietary fatty acids modulate plasma lipids[J]. Journal of Nutrition,2005,135(9):2075.
[26]Segner H, Verreth J. Metabolic enzyme activities in larvae of the African catfish, Clarias gariepinus: changes in relation to age and nutrition[J]. Fish Physiology Biochemistry,1995,14(5):385.
[27]向枭,周兴华,曾本和,等.蛋氨酸水平对罗非鱼蛋白质代谢及血清生化指标的影响[J].中国粮油学报,2016,31(11):92-98.
[28]Barcellos L J G, Kreutz L C, Souza C D, et al. Hematological changes in jundiá ( Rhamdia quelen, Quoy and Gaimard Pimelodidae ) after acute and chronic stress caused by usual aquacultural management, with emphasis on immunosuppressive effects[J]. Aquaculture,2004,237(1-4): 229-236.
[29]Authman M M, Abbas W T, Abumourad I M, et al. Effects of illegal cyanide fishing on vitellogenin in the freshwater African catfish, Clarias gariepinus (Burchell, 1822)[J]. Ecotoxicology Environmental Safety,2013,91(4):61.
[30]Smutna M, Vorlova L. Pathobiochemistry of ammonia in the internal environment of fish (Review)[J]. Acta Veterinaria Brno,2002,71(2):169-181.
[31]文远红,米海峰,张璐,等.棕榈油替代大豆油对吉富罗非鱼幼鱼生长性能、肌肉营养组成和血清生化指标的影响[J].动物营养学报,2016,28(3):953-960.
[32]Kenari A A, Mozanzadeh M T, Pourgholam R. Effects of total fish oil replacement to vegetable oils at two dietary lipid levels on the growth, body composition, haemato-immunological and serum biochemical parameters in caspian brown trout (Salmo trutta caspius Kessler, 1877)[J]. Aquaculture Research,2011,42(8):1131-1144.
[33]Kjaer M A, Vegusdal A, Gj?en T, et al. Effect of rapeseed oil and dietary n-3 fatty acids on triacylglycerol synthesis and secretion in Atlantic salmon hepatocytes[J]. Biochim Biophys Acta,2008,1781(3):112-122.
[34]邓君明.动植物蛋白源对牙鲆摄食、生长和蛋白质及脂肪代谢的影响[D].中国海洋大学,2006.
[35]Ritzhaupt L K, Bahr J M. A decrease in FSH receptors of granulosa cells during follicular maturation in the domestic hen[J]. Journal of Endocrinology,1987,115(2):303-310.
[36]Torstensen B E, ?yvind Lie, Fr?yland L. Lipid metabolism and tissue composition in Atlantic salmon (Salmo salar, L.)—Effects of capelin oil, palm oil, and oleic acid-enriched sunflower oil as dietary lipid sources[J]. Lipids,2000,35(6):653.
[37]何美莹,徐荣.橡胶种子、橡胶种子油的营养及食用安全性研究[J].热带农业科技,2013(3):1-6.
[38]满虹,钟国胜,D.D.Stosic.橡胶树籽是畜禽的良好饲料[J].中国畜牧兽医,1983(2):22-23.
[39]吴强强,李国富,吴江,等.牛至油对建鲤生长性能和血清抗氧化能力的影响[J].湖南农业科学,2010(5):136-138.
[40]赖建东,魏日凤,连玲丽,等.炭疽病病原菌侵染对茶树叶片生理特性的影响[J].福建农林大学学报(自然版),2016,45(2):162-168.
[41]赵珂立.氧化应激对动物自由基代谢、消化道损伤及抗氧化剂干预作用的研究[D].上海交通大学,2011.
[42]徐晓琴.血清谷胱甘肽过氧化物酶比色测定法[J].南京医科大学学报(自然科学版),1998(4):330-331.
[43]赵磊,龙晓文,吴旭干,等.育肥饲料中混合植物油替代鱼油对中华绒螯蟹成体雄蟹性腺发育、脂质代谢、抗氧化及免疫性能的影响[J].动物营养学报,2016(2):455-467.
[44]Hansen A C, Rosenlund G, ?rjan Karlsen, et al. Total replacement of fish meal with plant proteins in diets for Atlantic cod (Gadus morhua, L.) I-Effects on growth and protein retention[J]. Aquaculture,2007,272 (1):599-611.
[45]顾夕章,高启平,谢骏,等.饲料中亚麻籽替代部分大豆油对异育银鲫鱼苗生长性能、肌肉组成及血清生化指标的影响[J].动物营养学报,2012,24(11):2272-2278.
[46]Martins D A, Lmp V, Lall S P. Partial replacement of fish oil by flaxseed oil in Atlantic halibut (Hippoglossus hippoglossus L.) diets: effects on growth, nutritional and sensory quality[J]. Aquaculture Nutrition,2011,17(6):671-684.