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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (8): 50-53.doi: 10.11924/j.issn.1000-6850.casb15100133

• 食品 营养 检测 安全 • 上一篇    下一篇

超高效液相色谱法测定发芽糙米中γ-氨基丁酸含量

胡春苗1,2,陈丽娟2,3,谭亚玲2,3   

  1. (1云南农业大学稻作研究所,昆明 650201；2云南省高校滇型杂交粳稻分子育种重点实验室,昆明 650201；3云南农业大学农学与生物技术学院,昆明 650201）
  • 收稿日期:2015-10-30 修回日期:2015-12-21 接受日期:2015-12-25 出版日期:2016-03-18 发布日期:2016-03-18
  • 通讯作者: 陈丽娟
  • 基金资助:
    NSFC-云南联合基金重点项目“水稻雌配子育性基因的功能及其调控研究”(U11-36604)。

Determination of Gamma-aminobutyric Acid Content in Germinated Brown Rice by Ultra-high Performance Liquid Chromatography

Hu Chunmiao1,2, Chen Lijuan2,3, Tan Yaling2,3   

  1. (1Rice Research Institute, Yunnan Agricultural University, Kunming 650201;2Key Laboratory of Molecular Breeding of Japonica Hybrid Rice in Yunnan Province, Kunming 650201;3College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201)
  • Received:2015-10-30 Revised:2015-12-21 Accepted:2015-12-25 Online:2016-03-18 Published:2016-03-18

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摘要： 为发芽糙米中γ-氨基丁酸的含量检测提供高效的方法，采用超高效液相色谱柱Waters BEH C18(1.7 μm, 100 mm×2.1 mm)，以60%的乙醇为提取剂，流动相组成为乙腈和纯水，以邻苯二甲醛为衍生剂提对γ-氨基丁酸含量进行测定。该方法确定了样品最佳衍生反应时间为2 min，衍生物可在室温条件下稳定时间24 h以上或4℃低温保存。在此条件下绘制了γ-氨基丁酸的标准曲线，线性关系良好，检出限为0.5 mg/L，回收率范围98.88%~99.2%，相对标准偏差为0.6%~2.2%。该方法准确、灵敏、省时，为发芽糙米中γ-氨基丁酸的含量测定奠定了有效可靠的方法基础，可广泛应用。

关键词: 外源信号物质, 外源信号物质, 肉苁蓉, 种子萌发, 吸器形成, POD, CAT, ROS

Abstract: To provide an efficient method for the determination of gamma-aminobutyric acid (GABA) content in germinated brown rice, the content of GABA was determined by using ultra-high chromatographic column Waters BEH C18 (1.7 μm, 100 mm×2.1 mm) with 60% ethanol as the extraction agent, acetonitrile and water as mobile phase and OPA as the derivate agent. The results showed that the best derivation reaction time was 2 minutes, the sample could be stable for at least 24 hours under indoor temperature or stored at 4℃. The standard curve of GABA was plotted under the optimum condition. The mass concentration and the peak area of the low concentration standard sample showed good linear relationship, the detection limit was 0.5 mg/L, and the recovery rate was 98.88%-99.2% with the relative standard deviation of peak area being 0.6%-2.2%. The method is accurate, sensitive and time-saving, and can provide an effective and reliable tool for the determination of GABA in brown rice.