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中国农学通报 ›› 2021, Vol. 37 ›› Issue (21): 20-27.doi: 10.11924/j.issn.1000-6850.casb2020-0792

• 农学·农业基础科学 • 上一篇    下一篇

高铁胁迫对甜菜幼苗生长及保护系统的影响

耿贵1,2(), 王堽1, 董寅壮1, 范国凯2, 於丽华2, 王宇光2()   

  1. 1黑龙江大学生命科学学院,哈尔滨 150080
    2黑龙江大学现代农业与生态环境,哈尔滨 150080
  • 收稿日期:2020-12-15 修回日期:2021-02-05 出版日期:2021-07-25 发布日期:2021-07-29
  • 通讯作者: 王宇光
  • 作者简介:耿贵,男,1963年出生,黑龙江牡丹江人,博士生导师,研究员,博士,研究方向:植物生理生态学与甜菜栽培。通信地址:150080 黑龙江省哈尔滨市学府路74号 黑龙江大学,Tel:0451-86608913,E-mail: genggui01@163.com
  • 基金资助:
    国家自然科学基金“甜菜T510品系BvBHLH93转录因子功能及其耐盐调控机制分析”(31701487);国家糖料产业技术体系“甜菜种植制度”(CARS-170209);黑龙江省自然科学基金“转录因子BvWRKY62调控甜菜耐盐性的分子机理研究”(YQ2020037C)

Iron Enrichment and Sugar Beet Seedlings: Effect on the Growth and Protection System

Geng Gui1,2(), Wang Gang1, Dong Yinzhuang1, Fan Guokai2, Yu Lihua2, Wang Yuguang2()   

  1. 1College of Life Science, Heilongjiang University, Harbin 150080
    2College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
  • Received:2020-12-15 Revised:2021-02-05 Online:2021-07-25 Published:2021-07-29
  • Contact: Wang Yuguang

摘要:

为了探究高铁胁迫下甜菜幼苗生长和生理变化规律,本研究设置7个处理,即0.06、0.12、0.24、0.48、0.96、1.92、3.84 mmol/L,6次重复,并对甜菜进行水培试验。初步分析不同浓度Fe2+胁迫下甜菜幼苗中各项生理指标的变化。结果发现,随着Fe2+胁迫浓度的升高甜菜生长受抑制,地上部分叶面积变小,叶片相对电导率增大,相对含水量降低。地下部分表现出根系变短,面积变小,颜色变暗。与此同时高铁胁迫下甜菜植株抗氧化酶活性升高,参与渗透调节的可溶性糖和脯氨酸积累量均有不同程度的上升。变化趋势随着Fe2+的增加,先升高后降低,且在Fe2+胁迫浓度为0.96 mmol/L时达到最大值。说明甜菜植株抗氧化酶和渗透调节物质在高铁胁迫中起到了重要的调节作用,但当Fe2+超过一定浓度时,这种调节机制会明显受到抑制。

关键词: 甜菜, 铁胁迫, SOD, POD, APX, CAT, 渗透调节

Abstract:

To study the effect of iron enrichment on the growth and physiological changes of sugar beet seedlings, a hydroponic experiment was carried out with 7 treatments (0.06, 0.12, 0.24, 0.48, 0.96, 1.92 and 3.84 mmol/L with 6 repeats). The changes of various physiological indexes of sugar beet seedlings were determined and analyzed under different concentrations of Fe2+. The results showed that along with the increase of Fe2+ stress concentration, the growth of sugar beet was inhibited, the leaf area of the above-ground part decreased, the relative electrical conductivity of the leaf increased, and the relative water content decreased. The underground part showed that the root system became shorter, the area became smaller, and the color turned darker. At the same time, the antioxidant enzyme activity of sugar beet plants increased under high iron stress, and the accumulation of soluble sugar and proline involved in osmotic regulation increased to varying degrees. With the increase of Fe2+, the changing trend first increased and then decreased, and reached the maximum when the Fe2+ stress concentration was 0.96 mmol/L. The results indicate that the antioxidant enzymes and osmotic regulators of sugar beet plants play an important regulatory role in high concentration of iron stress, but when the Fe2+concentration exceeds a certain level, this regulatory mechanism could be significantly inhibited.

Key words: sugar beet, iron stress, SOD, POD, APX, CAT, osmotic regulation

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