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中国农学通报 ›› 2019, Vol. 35 ›› Issue (4): 136-142.doi: 10.11924/j.issn.1000-6850.casb18010160

• 食品 营养 检测 安全 • 上一篇    下一篇

栀子不同部位中栀子苷和西红花苷Ⅰ的提取工艺和含量测定

许林, 陈建荣   

  1. 湖南长沙开福区洪山路98号长沙学院生物与环境工程学院生物制药2班
  • 收稿日期:2018-01-30 修回日期:2019-01-04 接受日期:2018-04-04 出版日期:2019-01-31 发布日期:2019-01-31
  • 通讯作者: 陈建荣
  • 基金资助:
    湖南省自然科学基金项目“基于转录组的栀子萜类生物合成关键功能基因的挖掘”(2017JJ2294);湖南省科技创新计划(重点研发计划 2018NK2041)

Geniposide and Crocin Ⅰ in Different Parts of Gardenia: Extraction Technology and Content Determination

  • Received:2018-01-30 Revised:2019-01-04 Accepted:2018-04-04 Online:2019-01-31 Published:2019-01-31

摘要: 为优选栀子提取工艺,测定栀子不同部位中栀子苷和西红花苷Ⅰ含量,利用正交实验,采用超声、回流、微波3种提取方法对栀子不同部位栀子苷和西红花苷Ⅰ的提取工艺,同时建立两者的HPLC同步测定方法。结果表明,微波法的提取效果较好。果皮最佳提取工艺为提取温度为80℃,时间为25 min,料液比为1:15,乙醇浓度为95%;果仁最佳提取工艺为提取时间为30 min,料液比为1:20,温度为80℃,乙醇浓度为75%。采用Waters WATO54275-C18(4.6 mm×250 mm,5 μm)色谱柱,以乙腈-0.1%磷酸水为流动相,流速为1 mL/min,进行梯度洗脱,栀子苷和西红花苷Ⅰ检测波长分别为238、440 nm。HPLC测定结果表明,‘林海1号’栀子果皮中栀子苷、西红花苷Ⅰ最高含量分别为13.53、3.3 mg/g;果仁中栀子苷、西红花苷Ⅰ最高含量分别为44.64、7.72 mg/g。上述结果为栀子种质评价和不同部位的开发利用提供科学参考和依据。

关键词: WRF, WRF, 积云参数化方案, 微物理方案, 暴雨模拟

Abstract: To optimize the extraction process, we used an orthogonal experiment to evaluate the extraction of geniposide and crocin I from different parts of gardenia by ultrasonic, reflux and microwave methods, and at the same time, established an HPLC method for simultaneous determination of these two components. The results showed that the extraction effect of the microwave method was the best. The optimum extraction process from pericarp was: extraction temperature was 80℃, time was 25 min, the ratio of material to liquid was 1:15, and the concentration of ethanol was 95%. The optimum extraction process from kernel was: the extraction time of 30 min, the ratio of material to liquid of 1:20, the temperature of 80℃, and the concentration of ethanol of 75%. The HPLC separation was performed on a Waters WATO54275-C18 (4.6 mm×250 mm, 5 μm) column eluted with the mobile phase of acetonitrile-0.1% phosphoric acid, the flow rate was 1 mL/min. The detection wavelength of geniposide and crocin I was 238 and 440 nm. The HPLC results showed that the highest contents of geniposide and crocetin I in the pericarp of gardenia‘Linhai-1’was 13.53 and 3.3 mg/g, respectively. The highest contents of geniposide and crocetin I in the kernel of gardenia‘Linhai-1’was 44.64 and 7.72 mg/g, respectively. The above results have certain reference value for the germplasm evaluation and the development and utilization of different parts of gardenia.