灌施含Bacillus amyloliquefaciens SQR9复合微生物液体肥料在玉米和白菜上的促生效果研究

doi:10.11924/j.issn.1000-6850.casb18030090

中国农学通报 ›› 2019, Vol. 35 ›› Issue (24): 29-35.doi: 10.11924/j.issn.1000-6850.casb18030090

所属专题：玉米；园艺

灌施含Bacillus amyloliquefaciens SQR9复合微生物液体肥料在玉米和白菜上的促生效果研究

卢云峰, 许航, 斯天任, 高凯悦, 李荣, 沈宗专, 沈其荣

南京农业大学

收稿日期:2018-03-18 修回日期:2019-08-08 接受日期:2018-06-06 出版日期:2019-08-26 发布日期:2019-08-26
通讯作者: 李荣
基金资助:
国家重点研发计划“生物炭基肥料及微生物肥料研制”(2017YFD0200805)；江苏省科技计划项目“功能型生物育苗基质产业化关键技术研究”(BY2016077-05)；江苏高校品牌专业建设工程资助项目“农业资源与环境”(PPZY2015A061)；校大学生创新训练项目“养殖场废弃物资源化过程的理化与生物学耦合机制研究”(1713A24)。

Growth Promoting Effect of SQR9 Compound Microbial Liquid Fertilizer on Maize and Cabbage

Received:2018-03-18 Revised:2019-08-08 Accepted:2018-06-06 Online:2019-08-26 Published:2019-08-26

摘要/Abstract

摘要： [目的]明确自行研制的两种不同养分的含Bacillus amyloliquefaciens SQR9复合微生物液体肥料对玉米和白菜的促生效果,以期为新型生物水溶肥料的推广应用提供理论与实践指导。[方法]试验以玉米和白菜为供试材料,共设9个处理：灌施清水处理(CK)、灌施养分含量为100 g.kg-1的化肥处理(CF1)、灌施养分含量为100 g.kg-1的不添加Bacillus amyloliquefaciens SQR9的其他配方一样的对照水溶肥料处理(AA1)、灌施存储3个月后的养分含量为100 g.kg-1的含Bacillus amyloliquefaciens SQR9复合微生物液体肥料处理(AAS1-1)、灌施现配的养分含量为100 g.kg-1的含Bacillus amyloliquefaciens SQR9复合微生物液体肥料处理(AAS1-2)、灌施养分含量为200 g.kg-1的化肥处理(CF2)、灌施养分含量为200 g.kg-1的不添加Bacillus amyloliquefaciens SQR9的其他配方一样的对照水溶肥料处理(AA2)、灌施存储3个月后的养分含量为200 g.kg-1的含Bacillus amyloliquefaciens SQR9复合微生物液体肥料处理(AAS2-1)、灌施现配的养分含量为200 g.kg-1的含Bacillus amyloliquefaciens SQR9复合微生物液体肥料处理(AAS2-2)。[结果]盆栽试验结果表明,不论是在总养分100 g/kg施肥水平下,还是在总养分200 g/kg施肥水平下,相比于不添加Bacillus amyloliquefaciens SQR9的其他配方一样的对照水溶肥料处理、化肥和空白对照,含Bacillus amyloliquefaciens SQR9复合微生物液体肥料更能够促进玉米和白菜的生长,提高其生物学性状,但储存3个月后的和现配的含Bacillus amyloliquefaciens SQR9复合微生物液体肥料在玉米和白菜的生物学性状方面均无显著性差异；根际土壤中芽孢杆菌数量的测定结果表明,含功能菌复合微生物液体肥料的施用能够提高根际土壤中芽孢杆菌的数量。[结论]根部灌施含Bacillus amyloliquefaciens SQR9复合微生物液体肥料可以有效促进玉米和白菜的生长。

关键词: 最最高气温, 最最高气温, 数值天气预报, 多模式集合, 均方根误差, 预报准确率

Abstract: [Objective] This study aimed at evaluating the plant growth promotion effect of two different compound microbial liquid fertilizers containing Bacillus amyloliquefaciens SQR9 on maize and cabbage for providing the theoretical and technical base for the popularization and application of the novel products.[Method] Pot experiments were performed with nine treatments: fertigation of water (CK), fertigation of water soluble chemical fertilizer containing 100 g·kg-1 nutrients (CF1), fertigation of water soluble fertilizer containing 100 g·kg-1 nutrients without Bacillus amyloliquefaciens SQR9 (AA1), fertigation of compound microbial liquid fertilizer containing 100 g·kg-1 nutrients and Bacillus amyloliquefaciens SQR9 after 3 months of storage (AAS1-1), fertigation of freshly produced compound microbial liquid fertilizer containing 100 g·kg-1 nutrients and Bacillus amyloliquefaciens SQR9 (AAS1-2); fertigation of water soluble chemical fertilizer containing 200 g·kg-1 nutrients (CF2), fertigation of water soluble fertilizer containing 200 g·kg-1 nutrients without Bacillus amyloliquefaciens SQR9 (AA2), fertigation of compound microbial liquid fertilizer containing 200 g·kg-1 nutrients and Bacillus amyloliquefaciens SQR9 after 3 months of storage (AAS2-1), fertigation of freshly produced compound microbial liquid fertilizer containing 200 g·kg-1 nutrients and Bacillus amyloliquefaciens SQR9(AAS2-2). [Result]Pot experiment results showed regardless of the nutrient amounts, compared with water soluble fertilizer without Bacillus amyloliquefaciens SQR9, water soluble chemical fertilizer and the blank amended with water, the compound microbial liquid fertilizer containing Bacillus amyloliquefaciens SQR9 efficiently promoted maize and cabbage growth by improving their biological characters. No significant difference in the biological characters of maize and cabbage was observed between the water soluble fertilizers containing amino acids and Bacillus amyloliquefaciens SQR9 freshly produced and stored for 3 months; and application of water soluble fertilizer containing functional microbes increased the number of Bacillus in rhizosphere soil. [Conclusion]The compound microbial liquid fertilizer containing Bacillus amyloliquefaciens SQR9 effectively promoted the growth of maize and cabbage by root irrigation.

卢云峰,许航,斯天任,高凯悦,李荣,沈宗专,沈其荣. 灌施含Bacillus amyloliquefaciens SQR9复合微生物液体肥料在玉米和白菜上的促生效果研究[J]. 中国农学通报, 2019, 35(24): 29-35.

参考文献

[1] 栾江, 仇焕广, 井月, 等. 我国化肥施用量持续增长的原因分解及趋势预测[J]. 自然资源学报, 2013, 11(28): 1869-1878.
[2] 何元胜, 胡晓峰, 岳宁, 等. 微生物肥料的作用机理及应用前景[J]. 湖南农业科学, 2012 (10): 13-6.
[3] Blake L, Goulding K W T, Mott C J B, et al. Changes in soil chemistry accompanying acidification over more than 100 years under woodland and grass at Rothamsted Experimental Station UK[J]. European Journal of Soil Science, 1999, 50: 401-412..
[4] 吕家珑, 张一平, 王旭东, 等. 长期单施化肥对土壤性状及作物产量的影响[J]. 应用生态学报, 2001, 12(4): 569-572. .
[5] Yadav S N. Formulation and estimation of nitratenitrogen leaching from corn cultivation[J]. Journal of Environmental Quality, 1997, 26: 808-814
[6] 中华人民共和国农业部. NY/T 798-2015, 复合微生物肥料[S]
[7] 张玲, 李宝泽. 微生物肥料在果品生产中应用的潜力[J]. 北方果树, 2016(5): 1-3
[8] 孟瑶, 徐凤花, 孟庆有, 等. 中国微生物肥料研究及应用进展[J]. 中国农学通报, 2008, 24(6): 276-283.
[9] 许景钢, 孙涛, 李嵩. 我国微生物肥料的研发及其在农业生产中的应用[J]. 作物杂志, 2016(1): 1-6.
[10] 程乾斗, 王有科. 微生物肥料在农作物生产中的应用[J]. 现代园艺, 2013(1): 60-61.
[11] Gray E J, Smith D L. Intracellular and extracellular PGPR: commonalities and distinctions in the plant-bacterium signaling processes[J]. Soil Biology and Biochemistry, 2005, 37: 395-412
[12] 曹云. SQR9微生物有机肥防治黄瓜枯萎病的效应与机制研究[D].. 南京农业大学, 2011
[13] Cao Y, Zhang Z H, Ling N, et al. Bacillus subtilis SQR9 can control Fusarium wilt in cucumber by colonizing plant roots[J]. Biology and Fertility of Soils, 2011, 7: 495-506
[14] 张楠, 吴凯, 等. 根际益生菌解淀粉芽孢杆菌SQR-9在香蕉根表的定殖行为研究[J]. 南京农业大学学报, 2014, 37(6): 59-65
[15] 梁晓琳, 孙莉, 等. 利用Bacillus amyloliquefaciens SQR9研制复合微生物肥料[J]. 土壤, 2015, 47(3): 558-563
[16] 何欣, 郝文雅, 杨兴明, 等. 生物有机肥对香蕉植株生长和香蕉枯萎病防治的研究[J]. 植物营养与肥料学报, 2010, 16(4): 978－985
[17] Wang B B, Yuan J, Zhang J, et al. Effects of novel bioorganic fertilizer produced by Bacillus amyloliquefaciens W19 on antagonism of Fusarium wilt of banana[J]. Biology and Fertility of Soils, 2013, 49: 435-446
[18] Kinsella K, Shulthess C P, Morris T F, et al. Rapid quantification of bacillus subtilis in the rhizosphere[J]. soil biology and biochemistry, 2009, 41: 374-379
[19] 沈萍, 范秀容, 李广武主编. 微生物学实验(第三版)[M]. 北京: 高等教育出版社, 2002
[20] 龙彬, 万强, 周艳, 等. 生物有机–无机复合肥的研制及其在西瓜上的施用效果研究[J]. 湖南农业科学, 2008(5): 90–91
[21] 韩晓玲 , 张乃文, 贾敬芬. 生物有机无机复合肥对番茄产量、品质及土壤的影响[J]. 土壤肥料, 2005(3): 51–53
[22] ZHANG M, SHI J J, CAO L L, et al. Algal sludge from Taihu Lake can be utilized to create novel PGPR-containing bio-organic fertilizers[J].Journal of Environmental Management, 2014, 132: 230-236.
[23] 袁玉娟. Bacillus subtilis SQR9的黄瓜促生和枯萎病生防效果及其作用机制研究[D]. 南京农业大学, 2011.
[24] 张苗, 施娟娟, 曹亮亮, 等. 添加三种外源蛋白研制生物有机肥及其促生效果[J]. 植物营养与肥料学报, 2014, 20(5):1194 -1202.
[25] 巩子毓, 高旭, 黄炎, 等. 连续施用生物有机肥提高设施黄瓜产量和品质的研究[J]. 南京农业大学学报, 2016, 39(5) : 777－783
[26] Compant, S., Clément, C., Sessitsch, A. 2010. Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization[J]. Soil Biol. Biochem. 42, 669–678.
[27] Berg, G. 2009. Plant-microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture[J]. Appl. Microbiol. Biotechnol. 84, 11–18.
[28] Nsholm T, Ekblad A, Nordin A , et al. Boreal forest plants take up organic nitrogen[J]. Nature, 1998, 392: 914－916.
[29] Kinnersley A M, Robert C D, Kinnersely C Y, et al. Method for increasing fertilizer efficiency[J]. Unite States Patent: 5840656, 1998.
[30] 王蓓, 黄忠阳, 徐明喜, 等. 含氨基酸水溶肥料在设施辣椒和豇豆上的田间效应研究[J]. 土壤通报, 2017, 48(3): 683-691
[31] 王兰天. 含氨基酸水溶肥料在玉米和白菜上的应用效果研究[J]. 河南科学, 2013, 31(07): 972-974.

灌施含Bacillus amyloliquefaciens SQR9复合微生物液体肥料在玉米和白菜上的促生效果研究

Growth Promoting Effect of SQR9 Compound Microbial Liquid Fertilizer on Maize and Cabbage

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