复方中药免疫添加剂对施氏鲟生化指标的影响

doi:10.11924/j.issn.1000-6850.casb18070085

中国农学通报 ›› 2019, Vol. 35 ›› Issue (28): 129-136.doi: 10.11924/j.issn.1000-6850.casb18070085

复方中药免疫添加剂对施氏鲟生化指标的影响

冯淇元, 王荻, 刘红柏

中国水产科学研究院黑龙江水产研究所

收稿日期:2018-07-19 修回日期:2019-08-30 接受日期:2018-10-09 出版日期:2019-10-14 发布日期:2019-10-14
通讯作者: 刘红柏
基金资助:
现代农业产业技术体系建设专项资金(CARS-46)。

Effects of Chinese Herbal Compounds as Immune Additives on Biochemical Indexes of Acipenser schrenckii

Received:2018-07-19 Revised:2019-08-30 Accepted:2018-10-09 Online:2019-10-14 Published:2019-10-14

摘要/Abstract

摘要： [目的]为研究中药复方添加剂对施氏鲟的生化指标的影响,[方法]在水温17 ℃条件下,采用投喂饲料的方式研究了复方A(茵陈、板蓝根等)、复方B(三七、大黄等)、复方C(甘草、金银花等)对1+龄施氏鲟的生化指标的影响。每组200尾施氏鲟,三种中药复方分别按照2.0 %、1.0 %、2.5 %的质量比以液体的形式添加到基础饲料中。每天按照鱼体质量的2.5 %投喂制备的饲料,投喂等量的基础饲料作为对照组,连续饲养35天。待饲养结束后,每组取15尾施氏鲟,尾静脉采血,与采集的肝脏组织一同被检测。测定施氏鲟的血清、肝脏中活性物质含量和酶活性的变化。[结果]结果表明：复方A肝脏中白蛋白(ALB)含量高于对照组,其血清中ALB含量低于对照组。B组血清中ALB含量高于对照组,并且C组血清中ALB含量低于对照组但高于A组。A组肝脏中白蛋白与球蛋白比值(ALB/GLB)高于对照组。A组血清中尿素氮(BUN)含量低于对照组,B组和C组血清中BUN含量与对照组相比无差异。A组和C组血清中肌酐(CREA)含量低于对照组。B组血清中甘油三酯(TG)含量高于对照组。B组血清中乳酸脱氢酶(LDH)活性明显高于对照组,而A组和C组血清中LDH活性明显低于对照组。[结论]综上,说明复方B和复方C可能对机体造成一定损伤,需进一步研究适宜的给药剂量。而复方A按照原药占饲料2.0 %的质量比添加进饲料,对肝脏及肾脏具有一定保护作用,有助于提高施氏鲟的免疫能力。

关键词: 水浸泡法, 水浸泡法, 盆栽试验法, 氯乙烯-醋酸乙烯, 小麦-水稻产量

Abstract: [Objective]The objective of this paper is to study the effect of Chinese herbal compounds as immune additive on biochemical index in Amur sturgeon. [Method]The effects of Chinese herbal compound A (Herba artemisiae, Radix isatidis, etc.), Chinese herbal compound B (Notoginseng, Rhubarb, etc.) and Chinese herbal compound C (Liquorice, Honeysuckle etc.) on biochemical indicators of juvenile Amur sturgeons were studied by feeding fodders under the water temperature of 17 ℃. Each group has 200 Amur sturgeons and then the three traditional Chinese herbal compounds were added to the base feed by the quality of 2.0%, 1.0% and 2.5% in liquid form respectively. To feed the prepared feed daily to Amur sturgeons according to their body mass of 2.5 %, and the Amur sturgeons in the control group was given the same amount of basic feed for 35 d. After the drug administration, to collect the blood of vena caudalis from 15 Amur sturgeons in each group and the liver of Amur sturgeons were collected at the same time. The changes of the content of active substance and enzyme activity in serum and liver were determined. [Result]The results showed that the content of albumin (ALB) in liver of compound A was higher than which in the control group. And the content of ALB in serum of compound A was lower than which in the control group. The content of ALB in serum of compound B was higher than that in the control group. And the content of ALB in serum of compound C was lower than which in the control group but it was higher than which in the compound A. The ratio of albumin to globulin (ALB/GLB) in the liver of compound A was higher than that of the control group. The content of blood urea nitrogen (BUN) in serum of compound A was lower than that in the control group. There was no difference in the content of BUN in serum between compound B and compound C compared with the control group. The content of creatinine (CREA) in serum of compound A and compound C was lower than that in the control group. And the content of triglyceride (TG) in serum of compound B was higher than that in the control group. The activity of lactate dehydrogenase (LDH) in serum of compound B was significantly higher than that of the control group, while the activity of lactate dehydrogenase (LDH) in serum of compound A and compound C was significantly lower than that of the control group. [Conclusion]The study shows that compound B and compound C may cause certain damage to Amur sturgeon, and further study of appropriate dosages is needed. Compound A is added according to the weight of the original drug in the basic feed, which has a certain protective effect on the liver and kidney. And compound A is good to improve the immunity of Amur sturgeon.

中图分类号:

S942.5

冯淇元,王荻,刘红柏. 复方中药免疫添加剂对施氏鲟生化指标的影响[J]. 中国农学通报, 2019, 35(28): 129-136.

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复方中药免疫添加剂对施氏鲟生化指标的影响

Effects of Chinese Herbal Compounds as Immune Additives on Biochemical Indexes of Acipenser schrenckii

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