不同基因型甜菜根际土壤有机氮分布特征研究

doi:10.11924/j.issn.1000-6850.casb18090058

中国农学通报 ›› 2019, Vol. 35 ›› Issue (5): 107-114.doi: 10.11924/j.issn.1000-6850.casb18090058

所属专题：生物技术；园艺

• 资源环境生态土壤气象 • 上一篇下一篇

不同基因型甜菜根际土壤有机氮分布特征研究

王秋红¹, 周建朝¹, 王孝纯¹, 宋柏权^1,2, 邓艳红¹

1.黑龙江大学农作物研究院/中国农业科学院甜菜研究所;2.黑龙江省普通高校甜菜遗传育种重点实验室

收稿日期:2018-09-12 修回日期:2019-01-11 接受日期:2018-10-25 出版日期:2019-02-13 发布日期:2019-02-13
通讯作者: 周建朝
基金资助:
“十三五”国家糖料现代农业产业技术体系项目“甜菜养分管理与土壤肥料岗位”(CARS-170204)；黑龙江省科学基金项目“甜菜氮效率基因差异的生理机制研究”(C2018053)。

Distribution Characteristics of Soil Organic Nitrogen in Rhizosphere of Different Sugarbeet Genotypes

Received:2018-09-12 Revised:2019-01-11 Accepted:2018-10-25 Online:2019-02-13 Published:2019-02-13

摘要/Abstract

摘要： 摘要：土壤中除无机氮外，有机态氮也是植物生长发育的重要营养来源。为探明不同基因型甜菜对根际土壤有机氮吸收利用以及有机氮组分在根际的空间分布情况，分别选取对土壤有机氮吸收效率高、低的品种各1个，采用Kuchenbuch-周氏微型盆及田间网袋法，研究了甜菜苗期及大田全生育期根际土壤有机氮各组分的变化规律，建立了土壤氮矿化及各组分在根际空间分布的数学模型。结果表明：2周苗龄的甜菜根际土壤有机氮各组分含量高低顺序为：酸解总氮(ATN，acidolysis total nitrogen)>氨基酸态氮(AAN，amino acid nitrogen)>氨态氮(AN，ammonia nitrogen)>氨基糖态氮(ASN，amino sugar nitrogen)。其中AAN和AN在根际形成明显的耗竭面，即距离根表越远含量越高，在距根表15 mm时达到最高值，随后的变化渐趋平稳，其分布规律可用方程Y=Y0+Aln(X)拟合；ASN含量较低，随根际距离远近变化不显著；不同基因型甜菜对根际有机氮的耗竭能力各异，有机氮吸收效率高的品种均高于低的品种。田间自然条件下，甜菜根际土壤中的ATN、AAN及AN的含量在苗期增长最快，随后缓慢上升并逐渐趋于恒定。室内及田间试验结果表明，施氮处理后土壤有机氮的矿化量低于未施氮处理，其中有机氮吸收效率高的品种根际土壤有机氮的矿化量高于低的品种。

关键词: 白细胞介素2, 白细胞介素2, 重组杆状病毒, 联合免疫, 器官, 病理组织学

Abstract: Apart from inorganic nitrogen in soil, organic nitrogen is also an important nutrient source for plant growth and development. In order to ascertain the absorption and utilization of organic nitrogen in the rhizosphere and the spatial distribution of organic nitrogen components in the rhizosphere of different genotype sugar beet, one each of varieties with high and low organic nitrogen efficiency was selected. Using Kuchenbuch-Zhou''s miniature and field net bags, the variation regularities of organic nitrogen components in the rhizosphere of sugar beet seedlings and the whole growth period of the field were studied, and the mathematical model of soil nitrogen mineralization and the spatial distribution of each component in the rhizosphere was established. The results showed that the content of organic nitrogen in the rhizosphere soil of 2 weeks old seedlings was as follows: acid hydrolysis total nitrogen (ATN) > amino acid nitrogen (AAN) > ammonia nitrogen (AN) > amino sugar nitrogen (ASN). Among them, AAN and AN form obvious depletion surface in the rhizosphere, that is, the farther away from the root surface, the higher the content. The highest value is reached at 15mm from the root surface, and the subsequent changes become more stable. The distribution law can be obtained by the equation Y =Y0+Aln(X) fitting. The content of ASN is low, and it is not significant with the distance between the rhizosphere. The different sugarbeet varieties have different depletion ability to rhizosphere organic nitrogen, and the varieties with high organic nitrogen absorption efficiency are all Higher than the low variety. Under natural conditions in the field, the contents of ATN, AAN and AN in the rhizosphere soil of the beet grew fastest at the seedling stage, then slowly increased and gradually became constant. The results of laboratory and field experiments showed that the mineralization of soil organic nitrogen was lower than that of non-nitrogen treatment after nitrogen application, and the mineralization of rhizosphere soil organic nitrogen was higher than that of low variety.

王秋红,周建朝,王孝纯,宋柏权,邓艳红. 不同基因型甜菜根际土壤有机氮分布特征研究[J]. 中国农学通报, 2019, 35(5): 107-114.

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不同基因型甜菜根际土壤有机氮分布特征研究

Distribution Characteristics of Soil Organic Nitrogen in Rhizosphere of Different Sugarbeet Genotypes

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