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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (12): 40-45.doi: 10.11924/j.issn.1000-6850.casb18110121

所属专题： 油料作物

• 农学 农业基础科学 • 上一篇    下一篇

不同辐射强度对大豆M1代重要农艺性状的影响

齐波1, 汝玄玄1, 贾召召1, 霍晴1, 曹宇鹏1, 吴润之1, 李曙光2, 杨加银3,2   

  1. 1.淮阴工学院生命科学与食品工程学院;2.江苏徐淮地区淮阴农业科学研究所;3.淮安市农业科学研究院
  • 收稿日期:2018-11-28 修回日期:2018-12-15 接受日期:2018-12-24 出版日期:2019-04-26 发布日期:2019-04-26
  • 通讯作者: 杨加银
  • 基金资助:
    淮安市科技计划（指导性）项目“耐低温鲜食大豆新种质的创制与应用”(HANZ201603)；江苏省博士后科研资助项目“大豆籽粒蛋白质含量 功能型SNPs的发掘与候选基因的定位”(1701008C)；江苏省自然科学基金“大豆蛋白质含量的关联分析与相关基因的发掘”(BK20151285)；国家自然 科学基金“大豆种质群体蛋白质含量全基因组关联分析及优化组合设计育种效果的研究”(31571695)。

Radiation Intensities Affect Important Agronomic Characters of Soybean M1 Generation

  • Received:2018-11-28 Revised:2018-12-15 Accepted:2018-12-24 Online:2019-04-26 Published:2019-04-26

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摘要： 为研究不同强度的60Co γ射线对大豆重要农艺性状的辐照效应,明确适宜辐照剂量,本研究以淮豆13为材料,选取3种60Co γ射线辐照剂量(200 Gy、300 Gy和400 Gy)进行诱变处理。结果表明,不同辐照剂量均明显地降低了M1代群体的田间出苗率和成活率,且随着剂量的增加,出苗率和成活率不断降低,与对照(CK)相比,出苗率和成活率的变化幅度分别为44.34%-18.07%和24.45%-0.89%,其中400 Gy降幅最大；不同剂量处理对大豆始花期均有不同程度的延迟作用,比对照推迟3-5天开花,其中400 Gy对开花期影响最大,延迟时间达到5天；不同剂量处理对M1代群体株高、主茎节数、有效分枝数、单株荚数等重要农艺性状表现为剂量越高,表型差异越大；三种剂量中,300 Gy处理的M1代群体大小适中,且各种极端分化类型均具有一定的比例,表型变异较为丰富,获得目标性状的几率较大,适宜大豆突变体库构建。本研究结果为进一步构建不同类型的大豆突变体库以及深入开展功能基因组学研究提供了重要参考。

Abstract: To study the radiation effects of different intensities of 60Co γ- rays on important agronomic characters of soybean and determine the suitable radiation dose, soybean variety‘Huaidou 13’was used as material and radiated by using 200 Gy, 300 Gy and 400 Gy of γ- rays. The results showed that different radiation dosages significantly reduced the field seedling emergence rate and survival rate of M1 generation population. As the dosage increasing, the emergence rate and survival rate decreased. Compared with the control (CK), the variation range of emergence rate and survival rate was 18.07%-44.34% and 0.89%-24.45%, respectively. Meanwhile, radiation delayed the initial flowering stage of soybean from 3 to 5 days and the maximum delay effect showed up when treated with 400 Gy of radiation. By the same token, radiation affected some other important agronomic traits of M1 generation population, such as plant height, main stem number, effective branch number and pod number per plant, which turned out that the stronger of radiation intensity, the greater of difference in phenotype was. M1 generation group was moderate in size, extreme differentiation types had a certain proportion, phenotypic variation was abundant, and probability of obtaining target traits was high when treated with 300 Gy of radiation, which was suitable for the construction of soybean mutant library. The results of the study provide a foundation for constructing various types of soybean mutant libraries and further research on functional genomics.