茶树菇单孢菌株的单一性分析

doi:10.11924/j.issn.1000-6850.casb19010040

中国农学通报 ›› 2019, Vol. 35 ›› Issue (19): 68-72.doi: 10.11924/j.issn.1000-6850.casb19010040

所属专题：食用菌；食用菌；园艺

茶树菇单孢菌株的单一性分析

祝菱1,2,钱蓉1,2,赵永昌2,3,4,陈卫民2,3,4,陈玉惠1

1.云南省农业科学院生物技术与种质资源研究所;2.西南林业大学生命科学学院

收稿日期:2019-01-08 修回日期:2019-06-08 接受日期:2019-02-22 出版日期:2019-07-08 发布日期:2019-07-08
通讯作者: 陈玉惠
基金资助:
国家自然科学基金“信息素受体多等位基因在田头菇属真菌生物种识别中的作用研究”(31360024)；现代农业（食用菌）产业技术体系 (CARS20)。

The Singleness Analysis of Monokaryon from Agrocybe aegerita

Received:2019-01-08 Revised:2019-06-08 Accepted:2019-02-22 Online:2019-07-08 Published:2019-07-08

摘要/Abstract

摘要： 为了研究担子菌单核菌株单一性，通过形态与分子鉴定比对，以期为食用菌的杂交育种及遗传研究提供参考。用单孢稀释法涂布孢子，挑选单孢菌株进行锁状结构观察，判定的“单核菌株”用A和B交配位点进行扩增分析。结果表明：（1）随机挑选的11个单孢菌株中，10个菌株缺少该结构，被判定为单核体，而剩下的1个菌株具有明显的锁状结构，被判定为异核体；（2）依据交配位点A和B设计的引物扩增结果表明3个“单核体”为非单一性菌株，有两种A交配位点，但只有一种B交配位点。根据锁状结构认定的部分“单核体”可能会混有相同A或B交配型的异源菌株，建议单核体的分离采用单孢分离法，并且要结合分子鉴定。

Abstract: To study the singleness of monokayon from basidiomycetes, the morphology and molecular identification were compared for providing a reference for hybrid breeding and genetic research on edible fungi. The single-spore strain was isolated using the spore-dilution method, and then the clamp connection structure was observed. The decidable “monokayon” was further analyzed based on the amplification of A and B mating loci. The results showed: (1) Of 11 single-spore strains, 10 strains were identified for the monokaryon except 1 dikaryon producing clamp connection structure; (2) The amplified results based on the A and B mating loci showed 3 non-singleness “monokayons” contained two A and one B mating loci. The “monokaryotic strain” which identified by the clamp connection was probably mixed with heterogenous strain carrying the identical A or B mating loci, and it was suggested for obtaining monokaryotic strain using the single-spore isolation method combined with molecular analysis.

中图分类号:

S646.9

祝菱,钱蓉,赵永昌,陈卫民,陈玉惠. 茶树菇单孢菌株的单一性分析[J]. 中国农学通报, 2019, 35(19): 68-72.

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茶树菇单孢菌株的单一性分析

The Singleness Analysis of Monokaryon from Agrocybe aegerita

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