D-101大孔树脂对香椿叶中黄酮类成分的分离纯化及抗氧化活性研究

doi:10.11924/j.issn.1000-6850.casb20190800516

摘要/Abstract

摘要：

为充分利用香椿老叶,用D-101大孔吸附树脂对香椿叶中黄酮类成分进行分离纯化。以黄酮类成分的吸附率、洗脱率及黄酮纯度为指标,研究D-101大孔树脂对香椿中黄酮类成分的吸附能力及其稳定性。同时用清除1,1-二苯基苦基苯肼(DPPH)自由基的能力对香椿叶中黄酮类成分的抗氧化能力进行评价。结果表明,D-101大孔树脂的最佳上样条件为香椿浸提液上样量5 BV,黄酮类成分浓度0.69 mg/mL,流速3 BV/h。最适洗脱条件为70%乙醇洗脱剂,洗脱剂用量5 BV,流速3 BV/h。树脂使用1次时,可将香椿中黄酮类成分的纯度由6.0%提升到45.14%,且树脂稳定性良好,可重复使用6次后再生。经大孔树脂分离纯化后的黄酮类成分具有很强的抗氧化能力,为抗坏血酸的1.33倍。

关键词: 黄酮类成分, 分离纯化, D-101大孔吸附树脂, 香椿叶, 抗氧化活性

Abstract:

The flavonoids in leaves of Toona sinensis were separated and purified with D-101 macroporous resin to get the utmost out of the leaves. The adsorptive capacity and stability of D-101 macroporous resin for flavonoids from T. sinensis leaves were studied with the adsorption rate, elution rate and purity of total flavonoids as indexes. At the same time, the antioxidant activity of T. sinensis flavonoids was evaluated by scavenging 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radicals. The results showed that the optimum sampling condition of D-101 macroporous resin was: the sample size of T. sinensis extract was 5 BV, the flavonoids concentration was 0.69 mg/mL, and the sampling flow rate was 3 BV/h. The optimum elution condition was: 70% ethanol was used as eluent, the dosage of eluent was 5 BV, and the flow rate was 3 BV/h. When the resin was used once, the purity of flavonoids of T. sinensis could be increased from 6.0% to 45.14%, and the resin was stable and could be reused for 6 times before regeneration. The flavonoids isolated and purified by macroporous resin had strong antioxidant activity, which was 1.33 times as much as ascorbic acid.

Key words: flavonoids, separation and purification, D-101 macroporous resin, T. sinensis leaves, antioxidant activity

中图分类号:

TS202.3

邹鸿飞, 舒晓燕, 张月琴, 刘媛, 杨文玉, 赵桂英. D-101大孔树脂对香椿叶中黄酮类成分的分离纯化及抗氧化活性研究[J]. 中国农学通报, 2020, 36(24): 159-164.

Zou Hongfei, Shu Xiaoyan, Zhang Yueqin, Liu Yuan, Yang Wenyu, Zhao Guiying. Flavonoids of Toona sinensis Leaves: Separating and Purifying with D-101 Macroporous Adsorptive Resin and the Antioxidant Activity[J]. Chinese Agricultural Science Bulletin, 2020, 36(24): 159-164.

图/表 7

图1. 温度对大孔树脂吸附能力的影响

图2. 洗脱液浓度对大孔树脂洗脱率的影响

图3. 香椿黄酮泄漏曲线

图4. 上样浓度对黄酮吸附量的影响

图5. 流速对黄酮吸附率的影响

图6. 树脂脱附曲线

图7. 树脂使用稳定性考察

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