关键词: 哈蟆油蛋白多肽, 双酶法, 透皮作用, 分子质量, 黏度

Abstract:

A comparative study on relative molecular mass, viscosity and skin permeation effect of ranae oviductus peptides before and after enzymolysis was carried out. Ranae oviductus peptides were prepared through hydrolyzing ranae oviductus protein by pepsin and alkaline protease. The viscosimeter was used to detect the changes of viscosity during the enzymatic hydrolysis process. Taking the mouse skin as a transdermal barrier, percutaneous rate and the relative molecular weight distribution of percutaneous peptides at different times were detected by apparatus of transdermal absorption and high performance liquid chromatograph (HPLC), respectively. The results showed that the viscosity of ranae oviductus peptides decreased from 5278.85 mPa·s (1.0 g/100 mL) to 12.56 mPa·s (7.0 g/100 mL); the peak area percentage of ranae oviductus proteins with the relative molecular mass 1147 kDa went down from 75.36% to 8.83%, however, the percutaneous rate of ranae oviductus peptides increased from 4.50% to 9.14%. When the percutaneous time was 36 h, percutaneous peptides with the relative molecular mass less than 1 kDa were the main component in percutaneous fluid, accounting for about 62%, followed by percutaneous peptides with the relative molecular mass of 2.46 kDa, accounting for 13.34%. In general, the ranae oviductus peptides prepared by double-enzyme method have low molecular weight and good water solubility, and could penetrate the skin of mouse easily.

Key words: ranae oviductus peptides, double-enzyme method, skin permeation effect, molecular mass, viscosity