银芩麻杏石甘汤与桔芪麻杏石甘汤抗山羊支原体肺炎研究

doi:10.11924/j.issn.1000-6850.casb2021-0924

摘要/Abstract

摘要：

为了研究方剂对羊支原体肺炎的防治效果，将感染山羊支原体前后的羊采集静脉血，分离血清，用ELISA方法检测IL-10、IFN-γ和TNF-α含量。同时，每日观察临床症状，对迫杀的试验羊进行病理剖检和组织切片观察。结果显示：正常对照组未见异常，阳性对照组症状明显，死亡4只，方剂治疗组均比阳性对照组临床症状有明显的改善，银芩麻杏石甘汤中剂量组全部治愈。7天时阴性对照组与其他7组之间IL-10、IFN-γ和TNF-α差异均极显著(P<0.01)；14天时阴性对照组与其他7组差异极显著(P<0.01)，阳性对照组与6组中药组差异极显著(P<0.01)，银芩中剂量组与其他5组中药组差异显著(P<0.05)；21天时IL-10、IFN-γ阴性对照组与其他7组差异极显著(P<0.01)，阳性对照组与6组中药组差异极显著(P<0.01)，银芩中剂量组与其他5组中药组差异极显著(P<0.01)，银芩中剂量组与阴性对照组差异不显著(P>0.05)。21天时TNF-α阴性对照组与其他7组差异极显著(P<0.01)，阳性对照组与6组中药组差异极显著(P<0.01)，桔芪高剂量组与其他5组中药组差异极显著(P<0.01)，桔芪高剂量组与阴性对照组差异不显著(P>0.05)。由结果得到2个方剂均对治疗羊支原体肺炎有一定的效果，银芩麻杏石甘汤中剂量组对羊支原体肺炎的治疗效果最佳。

关键词: 羊支原体肺炎, 银芩麻杏石甘汤, 桔芪麻杏石甘汤, 细胞因子, 组织观察

Abstract:

To study the effect of prescriptions on mycoplasma pneumonia of goats, venous blood is collected from goat before and after the infection with Mycoplasma capricolumsubsp capricolum, the serum contents of IL-10, IFN-γ and TNF-α were detected by ELISA. At the same time, the clinical symptoms were observed daily, pathological necropsies and tissue section observation were performed on the goats. The results showed that normal control group had no abnormalities, the positive control group had obvious symptoms and 4 goats died. The clinical symptoms of the prescription treatment groups were significantly improved compared with those of the positive control group, and goats of Yinqin Maxingshigan decoction medium dose group were all cured. In terms of IL-10, IFN-γ and TNF-α, on the 7th day, there was significant difference between negative control group and other 7 groups (P<0.01); on the 14th day, there was significant difference between the negative control group and other 7 groups (P<0.01), significant difference between the positive control group and the 6 groups of Chinese medicine treatment (P<0.01), and significant difference between the medium dose group of Yinqin Maxingshigan decoction and other 5 groups of Chinese medicine treatment (P<0.05). As to IL-10 and IFN-γ, on the 21st day, there was significant difference between negative control group and other 7 groups (P<0.01), significant difference between the positive control group and the 6 groups of Chinese medicine treatment (P<0.01), significant difference between the medium dose group of Yinqin Maxingshigan decoction and other 5 groups of Chinese medicine treatment (P<0.01), and no significant difference between the medium dose group of Yinqin Maxingshigan decoction and negative control group (P>0.05). For TNF-α, on the 21st day, the negative control group had significant difference from the other 7 groups (P<0.01), there was significant difference between the positive control group and the 6 groups of Chinese medicine treatment (P<0.01), significant difference between the high dose group of Juqi Maxingshigan decoction and the other 5 groups of Chinese medicine treatment (P<0.01), and no significant difference between high dose group of Juqi Maxingshigan decoction and negative control group (P>0.05). Taken together, both of the two prescriptions have certain effects on mycoplasma pneumonia of goats, and the medium dose group of Yinqin Maxingshigan decoction has better effect.

Key words: mycoplasmal pneumonia of goats, Yinqin Maxingshigan decoction, Juqi Maxingshigan decoction, cytokines, tissue observation

中图分类号:

S858.26

解慧梅, 张君胜, 郭广富, 丁小丽, 高德丽, 孙胜钧. 银芩麻杏石甘汤与桔芪麻杏石甘汤抗山羊支原体肺炎研究[J]. 中国农学通报, 2022, 38(35): 97-104.

XIE Huimei, ZHANG Junsheng, GUO Guangfu, DING Xiaoli, GAO Deli, SUN Shengjun. Study on Anti- Mycoplasma capricolumsubsp capricolum by Yinqin Maxingshigan Decoction and Juqi Maxingshigan Decoction[J]. Chinese Agricultural Science Bulletin, 2022, 38(35): 97-104.

图/表 17

参考文献 29

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组别	0 d	7 d	14 d	21 d
阴性对照组	325.09±20.15^a	327.79±17.32^A	320.36±13.82^aA	320.44±13.73^A
阳性对照组	324.65±18.11^a	355.20±12.45^AB	380.85±15.49^dC	404.10±18.80^C
银芩低剂量	326.12±16.52^a	351.13±12.99 ^AB	351.13±12.99^cB	346.50±12.73^B
银芩中剂量	327.09±18.21^a	352.23±15.52 ^AB	334.82±11.22^bB	326.46±14.12^A
银芩高剂量	326.98±19.01^a	350.43±15.92 ^AB	355.79±13.62^cB	346.79±20.05^B
桔芪低剂量	325.66±16.68^a	351.85±15.72 ^AB	356.41±12.94^cB	357.42±15.45^B
桔芪中剂量	326.61±17.88^a	350.72±10.81 ^AB	351.35±11.64^cB	352.84±14.97^B
桔芪高剂量	327.31±18.32^a	350.19±11.98 ^AB	350.96±17.05^cB	347.56±15.26^B

组别	0 d	7 d	14 d	21 d
阴性对照组	325.09±20.15^a	327.79±17.32^A	320.36±13.82^aA	320.44±13.73^A
阳性对照组	324.65±18.11^a	355.20±12.45^AB	380.85±15.49^dC	404.10±18.80^C
银芩低剂量	326.12±16.52^a	351.13±12.99 ^AB	351.13±12.99^cB	346.50±12.73^B
银芩中剂量	327.09±18.21^a	352.23±15.52 ^AB	334.82±11.22^bB	326.46±14.12^A
银芩高剂量	326.98±19.01^a	350.43±15.92 ^AB	355.79±13.62^cB	346.79±20.05^B
桔芪低剂量	325.66±16.68^a	351.85±15.72 ^AB	356.41±12.94^cB	357.42±15.45^B
桔芪中剂量	326.61±17.88^a	350.72±10.81 ^AB	351.35±11.64^cB	352.84±14.97^B
桔芪高剂量	327.31±18.32^a	350.19±11.98 ^AB	350.96±17.05^cB	347.56±15.26^B

组别	0 d	7 d	14 d	21 d
阴性对照组	45.97±11.25^a	45.45±10.52^A	46.55±11.07^aA	46.50±11.11^A
阳性对照组	43.69±12.08^a	58.01±10.95^B	86.63±11.70^dC	99.76±10.01^D
银芩低剂量	45.76±11.09^a	58.02±11.42^B	61.19±11.72^cB	64.29±10.20C
银芩中剂量	44.67±12.12^a	56.57±10.17^B	55.05±11.18^bB	53.21±11.60^B
银芩高剂量	45.87±11.55^a	57.94±10.33^B	59.51±8.47^cB	63.15±11.09^C
桔芪低剂量	43.95±11.65^a	57.22±11.16^B	61.97±11.38^cB	62.92±11.83^C
桔芪中剂量	44.78±10.98^a	57.80±11.21^B	61.39±11.23^cB	67.63±10.94^C
桔芪高剂量	45.56±12.05^a	57.05±11.31^B	63.24±11.45^cB	58.26±10.51^C

组别	0 d	7 d	14 d	21 d
阴性对照组	45.97±11.25^a	45.45±10.52^A	46.55±11.07^aA	46.50±11.11^A
阳性对照组	43.69±12.08^a	58.01±10.95^B	86.63±11.70^dC	99.76±10.01^D
银芩低剂量	45.76±11.09^a	58.02±11.42^B	61.19±11.72^cB	64.29±10.20C
银芩中剂量	44.67±12.12^a	56.57±10.17^B	55.05±11.18^bB	53.21±11.60^B
银芩高剂量	45.87±11.55^a	57.94±10.33^B	59.51±8.47^cB	63.15±11.09^C
桔芪低剂量	43.95±11.65^a	57.22±11.16^B	61.97±11.38^cB	62.92±11.83^C
桔芪中剂量	44.78±10.98^a	57.80±11.21^B	61.39±11.23^cB	67.63±10.94^C
桔芪高剂量	45.56±12.05^a	57.05±11.31^B	63.24±11.45^cB	58.26±10.51^C

组别	0 d	7 d	14 d	21 d
阴性对照组	163.76±28.15^a	165.26±23.13^A	168.49±26.62^aA	169.02±29.09^A
阳性对照组	162.98±21.98^a	196.95±24.79^B	231.68±24.56^dC	223.50±23.55^C
银芩低剂量	164.56±22.76^a	197.20±25.29^B	209.52±23.53^cB	195.62±28.62^B
银芩中剂量	163.78±25.64^a	196.71±24.17^B	210.85±24.58^cB	189.46±23.25^B
银芩高剂量	165.12±24.09^a	196.81±24.16^B	204.69±28.98^cB	186.90±24.42^B
桔芪低剂量	163.89±22.33^a	196.96±28.66^B	211.92±23.71^cB	194.06±26.53^B
桔芪中剂量	164.65±26.23^a	197.12±23.86^B	210.11±22.14^cB	191.62±28.91^B
桔芪高剂量	163.88±24.69^a	196.28±27.06^B	185.71±30.06^bB	176.46±22.32^A