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中国农学通报 ›› 2022, Vol. 38 ›› Issue (23): 32-38.doi: 10.11924/j.issn.1000-6850.casb2022-0121

所属专题: 生物技术

• 林学·园艺·园林 • 上一篇    下一篇

外源硅对盐胁迫下黄果厚壳桂幼苗的光合荧光及抗氧化特性的影响

黄平升1(), 刘世男2, 李婷1, 覃永华1,3()   

  1. 1广西壮族自治区南宁树木园,南宁 530031
    2广西大学林学院,南宁 530004
    3广西壮族自治区林业勘测设计院,南宁 530011
  • 收稿日期:2022-03-02 修回日期:2022-05-07 出版日期:2022-08-15 发布日期:2022-08-08
  • 通讯作者: 覃永华
  • 作者简介:黄平升,男,1976年出生,广西容县人,工程师,硕士研究生,研究方向:林业经济管理与林业项目资金管理。通信地址:530031 广西南宁市江南区大王滩路1-18号南宁树木园,E-mail: 275313090@qq.com
  • 基金资助:
    广西林业科技推广示范项目(科学研究与技术攻关类)“黄果厚壳桂油材两用林经营及林产品开发”(桂林科研[2021]18号)

Effects of Exogenous Silicon on Photosynthesis and Chlorophyll Fluorescence Characteristics and Antioxidant Enzymes of Cryptocarya concinna Seedlings Under Salt Stress

HUANG Pingsheng1(), LIU Shinan2, LI Ting1, QIN Yonghua1,3()   

  1. 1Nanning Arboretum, Guangxi Zhuang Autonomous Region, Nanning 530031
    2Guangxi University, Forestry College, Nanning 530004
    3Guangxi Forestry Inventory and Planning Institute, Guangxi Zhuang Autonomous Region, Nanning 530011
  • Received:2022-03-02 Revised:2022-05-07 Online:2022-08-15 Published:2022-08-08
  • Contact: QIN Yonghua

摘要:

为探讨外源硅对盐胁迫下黄果厚壳硅的缓解作用。以一年生黄果厚壳桂(Cryptocarya concinna)幼苗为材料,在100 mg/L NaCl胁迫下,添加不同浓度(0、0.5、1、2 mmol/L)外源硅对幼苗进行处理,测定和分析不同处理下幼苗叶片的叶绿素含量、光合、叶绿素荧光特性参数和抗氧化酶含量。结果表明,外源硅能不同程度提高PnTrGsFv/Fm,降低Ci;提高叶绿素a、叶绿素b和类胡萝卜素含量;提高SOD、PPO和APX活性,降低MDA含量。其中PPO在1.0 mmol/L硅处理下效果最佳,其他指标均在 2.0 mmol/L硅处理效果最佳。可见,添加适度浓度硅可以缓解盐胁迫对黄果厚壳桂幼苗的伤害,提高其耐盐能力,该研究为黄果厚壳桂引种栽培提供参考。

关键词: 外源硅, 盐胁迫, 黄果厚壳硅, 光合荧光特性, 抗氧化酶

Abstract:

In this study,one-year-old seedlings of Cryptocarya concinna were treated with different concentrations (0, 0.5, 1 and 2 mmol/L) of exogenous silicon under salt stress at 100 mg/L NaCl. The chlorophyll content, photosynthesis and chlorophyll fluorescence characteristics and antioxidant enzymes were determined and analyzed for investigating the effects of exogenous silicon on C. concinna exposed to NaCl stress. The results indicated that exogenous silicon could increase the contents of chlorophyll a, chlorophyll b and carotenoid, the activities of SOD, PPO and APX, and the values of Pn, Tr, Gs and Fv/Fm, while reduce the MDA content and Ci value. By comparing different treatments, PPO content was the highest under the treatment of adding 1.0 mmol/L Si and other indexes were the highest under the treatment of adding 2.0 mmol/L Si. In conclusion, the addition of appropriate concentration of exogenous silicon can alleviate the damage of salt stress to C. concinna seedlings and improve seedling salt tolerance. This study will provide reference for the introduction and cultivation of C. concinna.

Key words: exogenous silicon, salt stress, Cryptocarya concinna, photosynthesis and chlorophyll fluorescence characteristics, antioxidant enzymes

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