高温胁迫对耐受性不同的家蚕品种幼虫抗氧化酶活与基因表达的影响

doi:10.11924/j.issn.1000-6850.casb2021-1144

中国农学通报 ›› 2022, Vol. 38 ›› Issue (35): 111-118.doi: 10.11924/j.issn.1000-6850.casb2021-1144

所属专题：生物技术

• 畜牧·动物医学·蚕·蜂 • 上一篇下一篇

高温胁迫对耐受性不同的家蚕品种幼虫抗氧化酶活与基因表达的影响

肖阳(), 李庆荣, 邢东旭, 杨琼()

广东省农业科学院蚕业与农产品加工研究所，广州 510610

收稿日期:2021-11-29 修回日期:2022-01-15 出版日期:2022-12-15 发布日期:2022-12-09
通讯作者: 杨琼
作者简介:肖阳，女，1982年出生，湖南长沙人，副研究员，硕士研究生，主要从事家蚕育种与分子机理方面的研究。通信地址：510610 广东省广州市天河区东莞庄一横路蚕业所，Tel：020-89282649，E-mail：xiaoyang@gdaas.cn。
基金资助:
国家自然科学基金项目“家蚕化学感受蛋白BmCSPs 基因在高温应答中的功能解析”(31802135);2022年省级乡村振兴战略专项资金种业振兴项目“家蚕核心种源创新培育提升与示范推广”;广东省现代农业产业技术体系（蚕桑）(2022KJ124);2022年度高水平广东省农业科技示范市建设资金市院合作项目“佛山基塘农业研究院”

Effects of High Temperature Stress on Antioxidant Enzyme Activity and Gene Expression in Larvae of Silkworm Varieties with Different Tolerance

XIAO Yang(), LI Qingrong, XING Dongxu, YANG Qiong()

Sericulture & Agri-food Research Institute, Guangdong Academy of Agriculture Sciences, Guangzhou 510610

Received:2021-11-29 Revised:2022-01-15 Online:2022-12-15 Published:2022-12-09
Contact: YANG Qiong

摘要/Abstract

摘要：

探讨高温环境对家蚕血淋巴中抗氧化酶活性及其基因表达的影响，为家蚕对高温环境胁迫的应答生理与分子适应机制的揭示提供参考。本实验以高温耐受性家蚕品种932G与敏感性品种HY为研究对象，采用实时荧光定量PCR等方法测定高温胁迫下5龄幼虫血淋巴中的抗氧化酶活性及酶基因表达水平的变化。实验结果表明，受35℃高温胁迫后，耐受性品种与敏感性品种血淋巴中SOD、CAT和GST的相对酶活力变化趋势相似，均为胁迫前期变化幅度较小，中后期显著升高，胁迫后期显著降低，整体变化趋势为先升高后降低。酶基因相对表达水平与酶活变化趋势基本一致，在胁迫中后期出现不同程度的升高，胁迫后期有所降低，但品种间变化幅度有差异，BmSOD与绝大多数BmGSTs基因的相对表达量以耐受性品种的升高幅度较大，而BmCAT则以敏感性品种的升高幅度较大。由此可见，抗氧化酶SOD、CAT和GST在蚕体血淋巴组织中的高温响应趋势较为一致，仅在品种间存在变化幅度的差异。

关键词: 高温胁迫, 耐受性, 抗氧化酶活, 基因表达, 家蚕

Abstract:

The effects of high temperature environment on antioxidant enzyme activity and gene expression in hemolymph of silkworm were explored, which may provide reference for revealing the physiological and molecular adaptation mechanism of silkworm to high temperature environmental stress. The tolerant variety to high temperature 932G and sensitive variety HY were taken as the studied objects, and the changes of antioxidant enzyme activities and enzyme gene expression levels in the hemolymph of the 5th instar larvae under high temperature stress were measured by real-time fluorescence quantitative PCR. The results showed that under 35℃ high temperature stress, the change trend of relative activities of SOD, CAT and GST in tolerant variety and sensitive variety were similar. The change ranges were all small in the early stage of stress, then increased significantly in the middle and late stages, and decreased significantly in the final stage of stress. The overall change trend was first increase then decrease. The relative expression levels of enzyme genes increased in varying degrees in the middle and late stages of stress, and decreased in the final stage of stress, which were basically consistent with the change trend of enzyme activities, but the change ranges among the varieties were different. The relative expression levels of BmSOD and most of BmGSTs genes increased significantly in tolerant variety, while that of BmCAT increased significantly in sensitive variety. In conclusion, the high temperature response trend of antioxidant enzymes SOD, CAT and GST in silkworm hemolymph are relatively consistent, and there are only differences in the change ranges among the varieties.

Key words: high temperature stress, tolerance, antioxidant enzyme activity, gene expression, Bombyx mori

中图分类号:

S881.24

肖阳, 李庆荣, 邢东旭, 杨琼. 高温胁迫对耐受性不同的家蚕品种幼虫抗氧化酶活与基因表达的影响[J]. 中国农学通报, 2022, 38(35): 111-118.

XIAO Yang, LI Qingrong, XING Dongxu, YANG Qiong. Effects of High Temperature Stress on Antioxidant Enzyme Activity and Gene Expression in Larvae of Silkworm Varieties with Different Tolerance[J]. Chinese Agricultural Science Bulletin, 2022, 38(35): 111-118.

图/表 4

参考文献 31

[1]	FELTON G W, SUMMERS C B. Antioxidant systems in insect[J]. Archives of insect biochemistry and physiology, 1995, 29(2):187-197. doi: 10.1002/arch.940290208 URL
[2]	JENA K, KAR P K, KAUSAR Z, et al. Effects of temperature on modulation of oxidative stress and antioxidant defenses in testes of tropical tasar silkworm Antheraea mylitta[J]. J therm biol, 2013, 38(4):199-204 doi: 10.1016/j.jtherbio.2013.02.008 URL
[3]	冯丽春, 沈卫德. 蚕体解剖生理学[M]. 北京: 高等教育出版社, 2015.
[4]	XIAO W F, CHEN P, XIAO J S, et al. Comparative transcriptome profiling of a thermal resistant vs. sensitive silkworm strain in response high temperature[J]. Plos one, 2017, 12(5):e0177641. doi: 10.1371/journal.pone.0177641 URL
[5]	李庆荣, 肖阳, 郑茜, 等. 不同家蚕品种血淋巴总SOD活力的差异及与生命力和产茧量性状的相关性[J]. 蚕业科学, 2012, 38(5):832-838.
[6]	袁燕萍, 赵林川, 魏广卫, 等. 家蚕品种7532和大造在高温冲击下中肠抗氧化酶活性的变化[J]. 蚕业科学, 2010, 36(4):692-696.
[7]	LI J X, LU Z T, MAO T T, et al. Identification of the nucleotide exchange factor BmGrpE and its role under high-temperature stress in silkworm, Bombyx mori[J]. Arch insect biochem physiol, 2020, 104(1):e21664.
[8]	JIANG L, HUANG C L, WANG B B, et al. Enhanced heat tolerance in transgenic silkworm via overexpression of Pyrococcus furiosus superoxide reductase[J]. Insect biochemistry and molecular biology, 2018, 92:40-44. doi: S0965-1748(17)30196-0 pmid: 29170068
[9]	WANG Y M, XIE E Y, GUO H Z, et al. Overexpression of Bmhsp19.9 protects BmE cells and transgenic silkworm against extreme temperatures[J]. International journal of biological macromolecules, 2020, 150:1141-1146. doi: S0141-8130(19)35815-5 pmid: 31739025
[10]	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J]. Methods, 2001, 25(4):402-408. doi: 10.1006/meth.2001.1262 URL
[11]	SUZUKI N, KOUSSEVITZKY S, MITTLER R, et al. ROS and redox signalling in the response of plants to abiotic stress[J]. Plant, cell & environ. 2012, 35, 259e270.
[12]	GENG X M, LIU X, JI M, et al. Enhancing heat tolerance of the little dogwood Cornus canadensis l. f. with introduction of a superoxide reductase gene from the hyperthermophilic archaeon Pyrococcus furiosus[J]. Front. plant sci. 2016, 7, 26.
[13]	APEL K, HIRT H. Reactive oxygen species: metabolism, oxidative stress, and signal transduction[J]. Annu. Rev. plant Biol. 2004, 55, 373e399.
[14]	IM Y J, JI M, LEE A, et al. Expression of pyrococcus furiosus superoxide reductase in Arabidopsis enhances heat tolerance[J]. Plant physiol, 2009, 151:893e904..
[15]	KAI H, HIRASHIMA K, MATSUDA O, et al. Thermotolerant cyclamen with reduced acrolein and methyl vinyl ketone[J]. J. exp. bot, 2012, 63, 4143e4150
[16]	CHOI Y S, LEE K S, YOON H J, et al. Bombus ignitus Cu,Zn superoxide dismutase (SOD1): cDNA cloning, gene structure, and up-regulation in response to paraquat, temperature stress, or lipopolysaccharide stimulation[J]. Comparative biochemistry and physiology. part b, 2006, 144(3):365-371. doi: 10.1016/j.cbpb.2006.03.014 URL
[17]	黄晓峰, 林夏丹, 朱倩婷, 等. 四种不同来源的超氧化物歧化酶(SOD)对黑腹果蝇寿命、繁殖力和抗逆能力的影响[J]. 昆虫学报, 2013, 56(7):765-771.
[18]	ENAYATI A A, RANSON H, HEMINGWAY J. Insect glutathione transferases and insecticide resistance[J]. Insect molecular biology, 2005, 14(1):3-8. pmid: 15663770
[19]	SHEEHAN D, MEADE Q, FOLEY V, et al. Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian menbers of an ancient enzyme superfamily[J]. Biochem.j, 2001, 360:1-16.
[20]	DUBOVSKIY I M, MARTEMYANOV V V, VORONTSOVA Y L, et al. Effect of bacterial infection on antioxidant activity and lipid peroxidation in the midgut of Galleria mellonella L. larvae (Lepidoptera, Pyralidae)[J]. Comparative biochemistry and physiology. part c, 2008, 148(1):1-5.
[21]	肖龙云, 程嘉翎, 王俊, 等. 家蚕氟化物中毒后血淋巴中脂质过氧化物和抗氧化物含量及抗氧化酶活性的变化[J]. 蚕业科学, 2010, 36(6):1047-1051.
[22]	杨伟克, 唐芬芬, 刘增虎, 等. 高温和低温条件下琥珀蚕血淋巴SOD及CAT活性的变化[J]. 江苏农业科学, 2017, 45(1):153-155.
[23]	贾姝, 赫英姿, 于庭洪, 等. 寄生线虫对柞蚕幼虫保护酶活性和免疫相关基因表达的影响[J]. 蚕业科学, 2020, 46(3):336-342.
[24]	唐芬芬, 杨伟克, 朱峰, 等. BmNPV对家蚕抗氧化酶基因表达及其酶活性的影响[J]. 南方农业学报, 2019, 50(10):2308-2313.
[25]	唐芬芬, 杨伟克, 朱峰, 等. 家蚕核型多角体病毒对家蚕酚氧化酶活性及其基因表达的影响[J]. 中国农学通报, 2016, 32(32):25-28.
[26]	MIAO Z Q, TU Y Q, GUO P Y, et al. Antioxidant enzymes and heat shock protein genes from Liposcelis bostrychophila are involved in stress defense upon heat shock[J]. Insects, 2020, 11:839. doi: 10.3390/insects11120839 URL
[27]	King A M, MacRae T H. Insect heat shock proteins during stress and diapause[J]. Annu. rev. entomol. 2015, 60:59-75. doi: 10.1146/annurev-ento-011613-162107 pmid: 25341107
[28]	崔娟. 筛豆龟蝽对温度变化的响应及其生理生化机制[D]. 长春: 吉林农业大学, 2019:68-98.
[29]	夏爱华, 李娜, 贾漫丽, 等. 高温胁迫对家蚕血液蛋白质组的影响[J]. 浙江农业学报, 2015, 27(7):1160-1167.
[30]	王晓迪, 冀顺霞, 申晓娜, 等. 温度胁迫下昆虫表观遗传机制的研究进展[J]. 中国生物防治学报, 2020, 37(3):598-608
[31]	CHEN P, XIAO W F, PAN M H, et al. Comparative genome-wide DNA methylation analysis reveals epigenomic differences in response to heat-humidity stress in Bombyx mori[J]. International journal of biological macromolecules, 2020, 164:3771-3779. doi: 10.1016/j.ijbiomac.2020.08.251 pmid: 32891645

基因	正向引物序列(5’-3’)	正向引物序列(5’-3’)
BmSOD	CAGCTGGAGCTCATTTCAAC	TAAAGTGCGACCAATGATGC
BmCAT	GACAGGGAGCGTATTCCAGA	CCACTCTCTCCACCAACTGT
BmGST 1(GSTe1)	CCTGAAGGCCTGGCCAAAAT	TAGGCAAGAGCGCATCCAAA
BmGST 2(GSTs1)	GGAAAGCTGACATGGGGTGA	AAGCCTTCACTTTGGGCTGT
BmGST 4(GSTz1)	CACTGGATCACAAGGGGCTT	CAGTTCACGGTCTATGCGGA
BmGST 5(GSTe2)	CGCAGATATCCAGGAAGCGT	ATCGACTGGCAACACACAGT
BmGST 6(GSTo2)	ACTGCCGCAAGATCCTTTGA	AGAACACAGTGCCTCGGTTT
BmGST 11(GSTe5)	TTGCCGGTGATGAGTTCTCC	ATCGGCAGTTGAGAGGAACG
BmGST 12(GSTe6)	TGATCAAGAACATCGAGGATGC	TCTGTTTATCCATTTGCTTTTCTCA
BmGST 13(GSTo4)	CGTGGCACAAGATTTCTCGG	GTATTGACTGACGGCGGGAT
BmGST d1	CTCAGCACACAATCCCGACT	GTCGATAATGGCACGTTGGC
BmGST d2	TGGTGAACAAGTACGCCAAAG	ACCTTCTCGTTCTTGGCCTTG
BmGST d3	CGCTTTGGACCTACAGCTCA	ACTGCTACTGTCTCCTCCGT
sw22934	TCCAAAAATGGGCCATCGAA	TGCTGGATTGCAGAAGGTTT

基因	正向引物序列(5’-3’)	正向引物序列(5’-3’)
BmSOD	CAGCTGGAGCTCATTTCAAC	TAAAGTGCGACCAATGATGC
BmCAT	GACAGGGAGCGTATTCCAGA	CCACTCTCTCCACCAACTGT
BmGST 1(GSTe1)	CCTGAAGGCCTGGCCAAAAT	TAGGCAAGAGCGCATCCAAA
BmGST 2(GSTs1)	GGAAAGCTGACATGGGGTGA	AAGCCTTCACTTTGGGCTGT
BmGST 4(GSTz1)	CACTGGATCACAAGGGGCTT	CAGTTCACGGTCTATGCGGA
BmGST 5(GSTe2)	CGCAGATATCCAGGAAGCGT	ATCGACTGGCAACACACAGT
BmGST 6(GSTo2)	ACTGCCGCAAGATCCTTTGA	AGAACACAGTGCCTCGGTTT
BmGST 11(GSTe5)	TTGCCGGTGATGAGTTCTCC	ATCGGCAGTTGAGAGGAACG
BmGST 12(GSTe6)	TGATCAAGAACATCGAGGATGC	TCTGTTTATCCATTTGCTTTTCTCA
BmGST 13(GSTo4)	CGTGGCACAAGATTTCTCGG	GTATTGACTGACGGCGGGAT
BmGST d1	CTCAGCACACAATCCCGACT	GTCGATAATGGCACGTTGGC
BmGST d2	TGGTGAACAAGTACGCCAAAG	ACCTTCTCGTTCTTGGCCTTG
BmGST d3	CGCTTTGGACCTACAGCTCA	ACTGCTACTGTCTCCTCCGT
sw22934	TCCAAAAATGGGCCATCGAA	TGCTGGATTGCAGAAGGTTT

基因	品种	高温胁迫时间/h
基因	品种	1	6	12	18	24	36	48
BmSOD	932G	1.04±0.04^C	0.76±0.09^D*	0.55±0.06^E*	1.00±0.09^C*	1.79±0.32^B*	5.54±0.48^A*	5.24±0.51^A*
BmSOD	HY	0.91±0.10^c	0.24±0.02^d	0.15±0.03^e	0.14±0.02^e	1.06±0.10^c	2.59±0.36^b	3.63±0.41^a
BmCAT	932G	0.93±0.05^B	0.82±0.09^B	0.24±0.09^C*	0.25±0.04^C*	0.32±0.04^C*	0.35±0.03^C*	1.10±0.09^A
BmCAT	HY	0.95±0.11^b	0.87±0.02^b	0.47±0.15^c	0.37±0.05^c	0.84±0.08^b	1.13±0.12^a	1.35±0.12^a
BmGST 1	932G	0.97±0.05^D	0.76±0.09^E	0.55±0.09^F*	0.40±0.04^G*	5.61±0.38^C*	9.19±0.82^B*	11.82±0.88^A*
BmGST 1	HY	1.31±0.11^f	1.22±0.02^f	1.89±0.15^e	2.71±0.28^d	22.20±3.05^c	35.19±2.28^a	28.17±1.96^b
BmGST 2	932G	0.93±0.03^D	1.60±0.12^C	2.80±0.23^B*	7.29±0.50^A*	6.84±0.59^A	4.16±0.43^B*	3.45±0.48^B*
BmGST 2	HY	1.04±0.28^e	1.36±0.15^de	1.54±0.16^d	1.82±0.11^c	6.96±0.10^b	11.05±0.82^a	7.33±0.49b
BmGST 4	932G	0.86±0.12^F	1.35±0.11^E*	2.56±0.28^D*	4.09±0.38^C*	5.26±0.45^B*	7.83±0.63^A*	7.65±0.32^A*
BmGST 4	HY	1.16±0.21^bc	0.98±0.07^c	1.28±0.13^b	1.32±0.15^b	1.52±0.14^ab	1.83±0.24^a	1.34±0.10^b
BmGST 5	932G	1.17±0.09^G	7.64±0.11^F*	24.08±0.90^E*	82.23±1.62^C*	105.73±2.82^A*	99.00±1.37^B*	76.20±1.08^D*
BmGST 5	HY	1.09±0.05^g	2.30±0.13^f	3.84±0.24^e	8.67±0.38^d	31.45±0.46^c	67.67±0.95^b	70.16±1.04^a
BmGST 6	932G	1.01±0.05^EF*	1.05±0.04^E*	0.94±0.05^F*	3.47±0.06^D*	6.82±0.09^B*	7.29±0.11^A*	4.11±0.05^C*
BmGST 6	HY	1.17±0.04^d	0.82±0.06^e	0.57±0.04^g	0.68±0.03^f	1.92±0.06^b	2.41±0.08^a	1.60±0.10^c
BmGST 7	932G	1.07±0.05^G*	4.24±0.06^F*	23.90±0.91^E*	96.56±1.42^D*	278.08±8.63^C*	497.83±12.59^A*	401.51±10.27^B*
BmGST 7	HY	0.86±0.05^g	6.04±0.12^f	27.31±1.01^e	75.33±1.58^d	102.96±3.65^c	156.52±5.02^a	138.28±8.48^b
BmGST 8	932G	0.93±0.02^D*	0.73±0.12^E	1.25±0.28^F*	1.41±0.29^G*	31.04±0.87^C*	68.38±1.54^A*	57.56±1.98^B*
BmGST 8	HY	0.99±0.02^d	0.75±0.03^f	0.86±0.02^e	0.88±0.04^e	14.46±0.66^c	34.22±1.05^a	28.29±0.83^b
BmGST 9	932G	1.06±0.05^F	1.16±0.06^F*	2.22±0.03^D*	1.53±0.02^E*	44.26±0.95^C*	137.00±4.83^A*	116.53±3.60^B*
BmGST 9	HY	0.97±0.04^d	0.83±0.02^e	0.78±0.05^e	0.56±0.03^f	26.62±0.73^c	37.36±0.77^a	32.37±1.28^b
BmGST 10	932G	1.03±0.06^G	2.35±0.27^F*	5.48±0.32^E*	11.62±0.36^D*	30.27±0.80^C*	39.54±1.02^A*	34.82±0.54^B*
BmGST 10	HY	1.01±0.03^d	0.88±0.01^e	0.32±0.02^f	0.16±0.01^g	12.78±0.45^c	48.92±1.03^a	25.26±0.86^b
BmGST 11	932G	1.02±0.02^D*	0.72±0.01^E	0.36±0.02^F*	0.12±0.01^G*	47.83±0.94^C*	169.53±4.37^A*	132.69±3.82^B*
BmGST 11	HY	1.03±0.02^e	0.72±0.02^f	0.66±0.01^g	1.31±0.04^d	17.74±0.65^c	61.25±2.72^a	47.08±1.59^b
BmGST 12	932G	0.92±0.03^F*	0.86±0.01^F*	1.44±0.04^E*	2.29±0.13^D*	5.84±0.20^A*	5.01±0.14^B*	3.47±0.09^C*
BmGST 12	HY	1.02±0.04^g	1.37±0.05^f	3.45±0.04^c	6.35±0.15^a	4.33±0.11^b	1.68±0.03^e	2.28±0.05^d
BmGST 13	932G	0.93±0.02^G	1.88±0.06^F	2.50±0.08^E*	7.37±0.18^D*	562.35±10.33^C*	3583.07±42.80^A*	1022.04±35.27^B*
BmGST 13	HY	0.98±0.03^g	1.64±0.02^f	2.27±0.09^e	5.89±0.16^d	103.36±1.08^c	247.71±4.64^a	198.18±5.29^b
BmGST d1	932G	0.97±0.05^F	1.36±0.06^D*	1.15±0.08^E*	0.83±0.02^G*	4.94±0.10^C*	25.36±0.62^B*	28.27±0.58^A*
BmGST d1	HY	1.03±0.03^g	0.89±0.02^f	2.33±0.05^e	4.46±0.08^d	9.52±0.17^c	33.41±0.88^a	22.73±0.92^b
BmGST d2	932G	1.02±0.01^F	1.11±0.05^E*	0.92±0.03^G*	2.48±0.07^D*	6.44±0.13^C*	29.77±0.46^A*	26.02±0.59^B*
BmGST d2	HY	1.02±0.02^c	0.78±0.01^d	0.21±0.01^f	0.03±0.00^g	0.57±0.01^e	1.40±0.03^b	1.86±0.05^a
BmGST d3	932G	0.98±0.02^D	1.03±0.01^C	0.91±0.02^E*	0.55±0.02^F*	1.03±0.04^CD*	2.05±0.05^B*	2.28±0.04^A
BmGST d3	HY	0.98±0.01d	0.52±0.02e	0.13±0.01g	0.26±0.02f	1.67±0.03c	3.39±0.24a	2.08±0.16b

基因	品种	高温胁迫时间/h
基因	品种	1	6	12	18	24	36	48
BmSOD	932G	1.04±0.04^C	0.76±0.09^D*	0.55±0.06^E*	1.00±0.09^C*	1.79±0.32^B*	5.54±0.48^A*	5.24±0.51^A*
BmSOD	HY	0.91±0.10^c	0.24±0.02^d	0.15±0.03^e	0.14±0.02^e	1.06±0.10^c	2.59±0.36^b	3.63±0.41^a
BmCAT	932G	0.93±0.05^B	0.82±0.09^B	0.24±0.09^C*	0.25±0.04^C*	0.32±0.04^C*	0.35±0.03^C*	1.10±0.09^A
BmCAT	HY	0.95±0.11^b	0.87±0.02^b	0.47±0.15^c	0.37±0.05^c	0.84±0.08^b	1.13±0.12^a	1.35±0.12^a
BmGST 1	932G	0.97±0.05^D	0.76±0.09^E	0.55±0.09^F*	0.40±0.04^G*	5.61±0.38^C*	9.19±0.82^B*	11.82±0.88^A*
BmGST 1	HY	1.31±0.11^f	1.22±0.02^f	1.89±0.15^e	2.71±0.28^d	22.20±3.05^c	35.19±2.28^a	28.17±1.96^b
BmGST 2	932G	0.93±0.03^D	1.60±0.12^C	2.80±0.23^B*	7.29±0.50^A*	6.84±0.59^A	4.16±0.43^B*	3.45±0.48^B*
BmGST 2	HY	1.04±0.28^e	1.36±0.15^de	1.54±0.16^d	1.82±0.11^c	6.96±0.10^b	11.05±0.82^a	7.33±0.49b
BmGST 4	932G	0.86±0.12^F	1.35±0.11^E*	2.56±0.28^D*	4.09±0.38^C*	5.26±0.45^B*	7.83±0.63^A*	7.65±0.32^A*
BmGST 4	HY	1.16±0.21^bc	0.98±0.07^c	1.28±0.13^b	1.32±0.15^b	1.52±0.14^ab	1.83±0.24^a	1.34±0.10^b
BmGST 5	932G	1.17±0.09^G	7.64±0.11^F*	24.08±0.90^E*	82.23±1.62^C*	105.73±2.82^A*	99.00±1.37^B*	76.20±1.08^D*
BmGST 5	HY	1.09±0.05^g	2.30±0.13^f	3.84±0.24^e	8.67±0.38^d	31.45±0.46^c	67.67±0.95^b	70.16±1.04^a
BmGST 6	932G	1.01±0.05^EF*	1.05±0.04^E*	0.94±0.05^F*	3.47±0.06^D*	6.82±0.09^B*	7.29±0.11^A*	4.11±0.05^C*
BmGST 6	HY	1.17±0.04^d	0.82±0.06^e	0.57±0.04^g	0.68±0.03^f	1.92±0.06^b	2.41±0.08^a	1.60±0.10^c
BmGST 7	932G	1.07±0.05^G*	4.24±0.06^F*	23.90±0.91^E*	96.56±1.42^D*	278.08±8.63^C*	497.83±12.59^A*	401.51±10.27^B*
BmGST 7	HY	0.86±0.05^g	6.04±0.12^f	27.31±1.01^e	75.33±1.58^d	102.96±3.65^c	156.52±5.02^a	138.28±8.48^b
BmGST 8	932G	0.93±0.02^D*	0.73±0.12^E	1.25±0.28^F*	1.41±0.29^G*	31.04±0.87^C*	68.38±1.54^A*	57.56±1.98^B*
BmGST 8	HY	0.99±0.02^d	0.75±0.03^f	0.86±0.02^e	0.88±0.04^e	14.46±0.66^c	34.22±1.05^a	28.29±0.83^b
BmGST 9	932G	1.06±0.05^F	1.16±0.06^F*	2.22±0.03^D*	1.53±0.02^E*	44.26±0.95^C*	137.00±4.83^A*	116.53±3.60^B*
BmGST 9	HY	0.97±0.04^d	0.83±0.02^e	0.78±0.05^e	0.56±0.03^f	26.62±0.73^c	37.36±0.77^a	32.37±1.28^b
BmGST 10	932G	1.03±0.06^G	2.35±0.27^F*	5.48±0.32^E*	11.62±0.36^D*	30.27±0.80^C*	39.54±1.02^A*	34.82±0.54^B*
BmGST 10	HY	1.01±0.03^d	0.88±0.01^e	0.32±0.02^f	0.16±0.01^g	12.78±0.45^c	48.92±1.03^a	25.26±0.86^b
BmGST 11	932G	1.02±0.02^D*	0.72±0.01^E	0.36±0.02^F*	0.12±0.01^G*	47.83±0.94^C*	169.53±4.37^A*	132.69±3.82^B*
BmGST 11	HY	1.03±0.02^e	0.72±0.02^f	0.66±0.01^g	1.31±0.04^d	17.74±0.65^c	61.25±2.72^a	47.08±1.59^b
BmGST 12	932G	0.92±0.03^F*	0.86±0.01^F*	1.44±0.04^E*	2.29±0.13^D*	5.84±0.20^A*	5.01±0.14^B*	3.47±0.09^C*
BmGST 12	HY	1.02±0.04^g	1.37±0.05^f	3.45±0.04^c	6.35±0.15^a	4.33±0.11^b	1.68±0.03^e	2.28±0.05^d
BmGST 13	932G	0.93±0.02^G	1.88±0.06^F	2.50±0.08^E*	7.37±0.18^D*	562.35±10.33^C*	3583.07±42.80^A*	1022.04±35.27^B*
BmGST 13	HY	0.98±0.03^g	1.64±0.02^f	2.27±0.09^e	5.89±0.16^d	103.36±1.08^c	247.71±4.64^a	198.18±5.29^b
BmGST d1	932G	0.97±0.05^F	1.36±0.06^D*	1.15±0.08^E*	0.83±0.02^G*	4.94±0.10^C*	25.36±0.62^B*	28.27±0.58^A*
BmGST d1	HY	1.03±0.03^g	0.89±0.02^f	2.33±0.05^e	4.46±0.08^d	9.52±0.17^c	33.41±0.88^a	22.73±0.92^b
BmGST d2	932G	1.02±0.01^F	1.11±0.05^E*	0.92±0.03^G*	2.48±0.07^D*	6.44±0.13^C*	29.77±0.46^A*	26.02±0.59^B*
BmGST d2	HY	1.02±0.02^c	0.78±0.01^d	0.21±0.01^f	0.03±0.00^g	0.57±0.01^e	1.40±0.03^b	1.86±0.05^a
BmGST d3	932G	0.98±0.02^D	1.03±0.01^C	0.91±0.02^E*	0.55±0.02^F*	1.03±0.04^CD*	2.05±0.05^B*	2.28±0.04^A
BmGST d3	HY	0.98±0.01d	0.52±0.02e	0.13±0.01g	0.26±0.02f	1.67±0.03c	3.39±0.24a	2.08±0.16b

高温胁迫对耐受性不同的家蚕品种幼虫抗氧化酶活与基因表达的影响

Effects of High Temperature Stress on Antioxidant Enzyme Activity and Gene Expression in Larvae of Silkworm Varieties with Different Tolerance

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