耐镉根际促生菌WYN5的分子鉴定及其对黑麦草富集镉的影响

doi:10.11924/j.issn.1000-6850.casb2022-0711

摘要/Abstract

摘要：

研究旨在为植物-微生物联合修复提供优异菌种资源及技术支撑。以筛选的高耐镉菌株WYN5为试材，测定该菌株体外促生长能力，并通过生理生化实验，结合菌株形态及系统发育分析进行菌株鉴定；在水培试验中，当镉胁迫质量浓度为0~10 mg/L时，接种菌株WYN5均能促进黑麦草株高、根长、根部干质量和茎叶部干质量的增长，同质量浓度的镉与对照相比，WYN5处理分别提高了26.2%~28.0%、15.0%~50.7%、12.5%~66.7%和10.5%~20.8%。各处理的根部和茎叶镉含量也均呈增加趋势，同质量浓度的镉与对照相比，WYN5处理的根部镉浓度提高了25.1%~128.7%，且均达到显著水平(P<0.05)；茎叶部镉浓度提高了40.8%~64.9%；接种菌株WYN5能够促使黑麦草富集镉，促进镉从根部向茎叶部的转运，在植物-微生物联合修复镉污染土壤中具有一定的应用潜力。

关键词: 耐镉菌株, 黑麦草, 促生长, 水培试验, 镉污染土壤

Abstract:

The aim is to provide excellent strain resources and technical support for plant-microbial joint remediation. The cadmium-tolerant strain WYN5 was selected as the test material to determine the growth promotion ability of the strain in vitro. The strain was identified by physiological and biochemical tests combined with morphology and phylogenetic analysis. In the hydroponic experiment, inoculated strain WYN5 could promote the increase of plant height, root length, dry weight of root, stem and leaf when the cadmium concentration was 0-10 mg/L. WYN5 treatments increased by 26.2%-28.0%, 15.0%-50.7%, 12.5%-66.7% and 10.5%-20.8%, respectively. Cadmium content in roots, stems and leaves of all treatments also showed an increasing trend. Compared with the control, cadmium concentration in roots of WYN5 treatment was increased by 25.1%-128.7%, and reached a significant level (P < 0.05). Cadmium concentration in stems and leaves was increased by 40.8%-64.9%. Inoculated strain WYN5 can promote the enrichment of cadmium in ryegrass and promote the transfer of cadmium from roots to stems and leaves, which has certain application potential in the remediation of cadmium-contaminated soil by plant-microorganism combination.

Key words: cadmium-tolerant strain, ryegrass, growth promotion, hydroponic experiment, cadmium-contaminated soil

杜东霞, 李咏梅, 喻孟元, 王震, 许丽娟, 吴民熙. 耐镉根际促生菌WYN5的分子鉴定及其对黑麦草富集镉的影响[J]. 中国农学通报, 2023, 39(27): 59-66.

DU Dongxia, LI Yongmei, YU Mengyuan, WANG Zhen, XU Lijuan, WU Minxi. Cadmium-tolerant Rhizosphere Growth-promoting Bacteria WYN5: Molecular Identification and Its Effects on Cadmium Enrichment in Ryegrass[J]. Chinese Agricultural Science Bulletin, 2023, 39(27): 59-66.

图/表 11

参考文献 19

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菌株编号	吲哚乙酸产生	铁载体产生	溶磷	溶钾	固氮	耐镉/(mg/L)
WYN1	+	+	-	-	-	220
WYN3	+	-	-	+	-	210
WYN4	-	-	-	+	-	230
WYN5	+	+	+	+	+	230
WYN7	+	+	-	-	+	220
WYN9	-	-	+	+	-	230
WYN10	-	-	-	-	-	200
WYN11	+	-	-	-	-	210
WYN20	-	+	+	+	-	210

菌株编号	吲哚乙酸产生	铁载体产生	溶磷	溶钾	固氮	耐镉/(mg/L)
WYN1	+	+	-	-	-	220
WYN3	+	-	-	+	-	210
WYN4	-	-	-	+	-	230
WYN5	+	+	+	+	+	230
WYN7	+	+	-	-	+	220
WYN9	-	-	+	+	-	230
WYN10	-	-	-	-	-	200
WYN11	+	-	-	-	-	210
WYN20	-	+	+	+	-	210

实验项目	结果	实验项目	结果	实验项目	结果
阴性对照	-	D-海藻糖	+	D-葡糖酸	+
丙酮酸甲酯	+	蔗糖	+	糖质酸	-
溴代丁二酸	+	水苏糖	+	D-棉子糖	+
α-D-葡萄糖	+	龙胆二糖	+	α-D-乳糖	+
D-甘露糖	+	L-丙氨酸	+	L-乳酸	+
D-果糖	+	L-精氨酸	+	柠檬酸	+
D-半乳糖	+	L-天冬氨酸	+	D-苹果酸	+
D-麦芽糖	+	L-谷氨酸	+	吐温40	+
γ-氨基丁酸	+	α-羟丁酸	+	乙酰乙酸	+
丙酸	+	乙酸	+	甲酸	+

实验项目	结果	实验项目	结果	实验项目	结果
阴性对照	-	D-海藻糖	+	D-葡糖酸	+
丙酮酸甲酯	+	蔗糖	+	糖质酸	-
溴代丁二酸	+	水苏糖	+	D-棉子糖	+
α-D-葡萄糖	+	龙胆二糖	+	α-D-乳糖	+
D-甘露糖	+	L-丙氨酸	+	L-乳酸	+
D-果糖	+	L-精氨酸	+	柠檬酸	+
D-半乳糖	+	L-天冬氨酸	+	D-苹果酸	+
D-麦芽糖	+	L-谷氨酸	+	吐温40	+
γ-氨基丁酸	+	α-羟丁酸	+	乙酰乙酸	+
丙酸	+	乙酸	+	甲酸	+

实验项目	结果	实验项目	结果	实验项目	结果
阳性对照	+	1%NaCl	+	萘啶酸	+
利福霉素SV	+	4%NaCl	+	氯化锂	+
十四烷硫酸钠	-	8%NaCl	+	亚碲酸钾	+
1%乳酸钠	+	pH 6.0	+	溴酸钠	+
D-丝氨酸	+	pH 5.0	+	丁酸钠	+