欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (11): 134-141.doi: 10.11924/j.issn.1000-6850.casb2023-0412

所属专题： 植物保护

• 植物保护 • 上一篇    下一篇

肺形侧耳和白星花金龟幼虫对11种林果枝条废弃物联合转化能力比较

赵威(), 赵传岳, 孔昊, 谢兴同, 王丽()   

  1. 山东农业大学植物保护学院山东省农业微生物重点实验室，山东泰安 271018
  • 收稿日期:2023-05-31 修回日期:2023-08-23 出版日期:2024-04-15 发布日期:2024-04-11
  • 通讯作者:
    王丽，女，1985年出生，山东泰安人，副教授，博士，研究方向：以食用菌为纽带的农林废弃物的资源化利用。通信地址：271018 山东泰安山东农业大学岱宗校区植物保护学院 山东省农业微生物重点实验室，Tel：0538-8242375，E-mail：。
  • 作者简介:

    赵威，男，1999年出生，山东菏泽人，在读硕士，研究方向：以食用菌为纽带的农林废弃物的资源化利用。通信地址：271018 山东泰安山东农业大学岱宗校区植物保护学院 山东省农业微生物重点实验室，E-mail：。

  • 基金资助:
    自治区重点研发计划项目“吨量级白星花金龟规模化养殖技术”(2022B02046); 山东省现代农业产业技术体系食用菌创新团队建设项目(SDAIT-07-06)

Comparison of Combined Transformation Ability of Pleurotus pulmonarius and Protaetia brevitarsis Larvae to 11 Kinds of Forest Fruit Branch Waste

ZHAO Wei(), ZHAO Chuanyue, KONG Hao, XIE Xingtong, WANG Li()   

  1. Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai’an, Shandong 271018
  • Received:2023-05-31 Revised:2023-08-23 Published:2024-04-15 Online:2024-04-11

RichHTML

0

PDF (PC)

13

摘要：

为了探究腐生型食用菌——肺形侧耳和腐食性昆虫——白星花金龟幼虫对11种林果木枝条废弃物的联合转化能力，首先利用肺形侧耳PL2菌株对不同林果木枝条进行生物转化，测定其菌丝生长速度、子实体产量、子实体多糖含量以及子实体浸提液的抗氧化活性；之后采用白星花金龟幼虫对基于11种林果木枝条的肺形侧耳菌糠进行生物转化，并使用Hassan方法综合评价了肺形侧耳与白星花金龟幼虫对11种林果木枝条的联合转化效果。结果显示，不同种类的林果木枝条对肺形侧耳菌株的菌丝生长速度、子实体产量、子实体多糖含量以及子实体抗氧化活性均产生显著差异，在榉木中菌丝生长速度最快(10.05 mm/d)，在金银花枝条中生物学效率最高(68.95%)，在桃木中多糖含量最高(9.52%)，在杨木中综合抗氧化性最强(OD 0.6409)。进一步研究发现，肺形侧耳与白星花金龟幼虫对11种林果木枝条的联合转化效果存在显著性差异，其中对金银花枝条的联合转化效果最优，1 kg金银花枝条可产生846.19 g肺形侧耳子实体，使白星花金龟幼虫虫体增重45.97 g，产生499.54 g虫砂。本研究结果表明，肺形侧耳与白星花金龟幼虫对林果木枝条废弃物的联合转化效果显著，具有一定的应用价值，可为林果木枝条废弃物资源的生态高值化利用提供理论依据。

关键词: 林果枝条, 肺形侧耳, 白星花金龟幼虫, 生物转化

Abstract:

In order to explore the combined transformation ability of saprophytic edible fungus-Pleurotus pulmonarius and saprophytic insect- Protaetia brevitarsis larvae (PBL) to 11 kinds of forest and fruit tree branch waste, P. pulmonarius PL2 strain was used to biotransform different forest and fruit tree branches, and the mycelial growth rate, fruiting body yield, fruiting body polysaccharide content and antioxidant activity of fruiting body extract were determined. After that, the spent substrate of P. pulmonarius based on 11 kinds of wood branches was biotransformed by PBL, and the Hassan method was used to comprehensively evaluate the combined transformation effect of P. pulmonarius and PBL on 11 kinds of wood branches. The results showed that there were significant differences in the mycelial growth rate, fruiting body yield, fruiting body polysaccharide content and fruiting body antioxidant activity of P. pulmonarius strains among different types of branches. The mycelial growth rate was the fastest in beech wood (10.05 mm/d), the biological efficiency was the highest in honeysuckle branches (68.95%), the polysaccharide content was the highest in peach wood (9.52%), and the comprehensive antioxidant activity was the strongest in poplar wood (OD 0.6409). Further studies showed that there were significant differences in the combined transformation effect of P. pulmonarius and PBL on 11 kinds of branches. Among them, the combined transformation effect on honeysuckle branches was the best. 1 kg honeysuckle branches could produce 846.19 g of P. pulmonarius fruiting bodies. The weight gain of PBL was 45.97 g, and the amount of larvae frass production was 499.54 g. The results of this study showed that the combined conversion effect of P. pulmonarius and PBL on forest fruit tree branch waste was significant and had certain application value, which could provide theoretical basis for the ecological high-value utilization of forest fruit tree branch waste resources.

Key words: forest and fruit trees sawdust, Pleurotus pulmonarius, Protaetia brevitarsis larvae (PBL), biotransformation