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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (2): 123-132.doi: 10.11924/j.issn.1000-6850.casb2024-0100

• 植物保护 • 上一篇    下一篇

取食银杏的粗狭肋鳃金龟肠道可培养细菌的分离鉴定研究

陈杰1(), 龙婷1, 曹易蓉1, 孙淦琳1, 王文倩1, 申开元2, 金思同1, 蔡浩升1, 陈斌1()   

  1. 1 云南农业大学植物保护学院/云南生物资源保护与利用国家重点实验室，昆明 650201
    2 云南希美康农业开发有限公司，云南曲靖 655500
  • 收稿日期:2024-02-06 修回日期:2024-06-03 出版日期:2025-01-13 发布日期:2025-01-13
  • 通讯作者:
    陈斌，男，教授，博士，研究方向：害虫综合治理研究。E-mail：。
  • 作者简介:

    陈杰，男，2000年出生，硕士研究生，研究方向：昆虫生理。E-mail：。

  • 基金资助:
    云南省科技厅科技计划项目“云南省文山市林下三七产业科技特派团(202404BI090010); 国家自然科学基金项目C-型草地贪夜蛾幼虫肠道细菌多样性及寄主适应机理研究(32060616); 云南省教育厅创新团队项目云南省教育厅害虫生物防治科技创新团队(2022[69])

Isolation and Identification of Culturable Intestinal Bacteria of Holotrichia scrobiculata Brenske Feeding on Ginkgo biloba

CHEN Jie1(), LONG Ting1, CAO Yirong1, SUN Ganlin1, WANG Wenqian1, SHEN Kaiyuan2, JIN Sitong1, CAI Haosheng1, CHEN Bin1()   

  1. 1 College of Plant Protection, Yunnan Agricultural University / State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Kunming 650201
    2 Yunnan Ximeikang Agricultural Development Co., Ltd, Qujing, Yunnan 655500
  • Received:2024-02-06 Revised:2024-06-03 Published:2025-01-13 Online:2025-01-13

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摘要：

对取食银杏叶的粗狭肋鳃金龟成虫肠道可培养细菌进行分离鉴定，为粗狭肋鳃金龟寄主适应性机制研究及综合防治提供理论依据。分别采用LB、NA和TYA培养基分离培养，结合16S rDNA序列分析，对取食银杏叶的粗狭肋鳃金龟雌性和雄性成虫肠道可培养细菌进行分离、培养和鉴定，探究取食银杏叶的粗狭肋鳃金龟肠道可培养细菌物种多样性。粗狭肋鳃金龟雌性和雄性成虫肠道可培养细菌组成结构不同，其中雌成虫肠道可培养细菌有4门11科13属14种，优势菌门、科、属和种细菌分别为厚壁菌门Firmicutes、芽孢杆菌科Bacillaceae、芽孢杆菌属Bacillus、枯草芽孢杆菌Bacillus subtilis，其丰度分别为86.58%、86.56%、62.59%和61.64%；雄成虫肠道可培养细菌有3门6科7属11种，优势菌门、科、属和种分别为厚壁菌门Firmicutes、芽孢杆菌科Bacillaceae、片球菌属Pediococcus、戊糖片球菌Pediococcuspentosaceus，丰度分别为98.64%、37.74%、34.68%和34.68%。从优势种细菌来看，雌虫肠道优势种细菌为枯草芽孢杆菌Bacillus subtilis，丰度为61.64%，而雄虫肠道优势种细菌为高山芽孢杆菌Bacillus altitudinis和戊糖片球菌Pediococcuspentosaceus，相对丰度分别为31.91%和34.68%。粗狭肋鳃金龟雌性和雄性成虫肠道共有的可培养细菌有2种，分别为戊糖片球菌Pediococcuspentosaceus和Sphingomonashumi。取食银杏叶片的粗狭肋鳃金龟成虫肠道可培养细菌物种多样性丰富，肠道细菌物种组成随雌、雄性别的不同而存在差异，优势种也不同。

关键词: 粗狭肋鳃金龟, 银杏, 肠道细菌, 可培养细菌, 16S rDNA序列分析, 物种多样性

Abstract:

Isolation and identification of culturable bacteria in the intestinal tract of Holotrichia scrobiculata Brenske adults feeding on Ginkgo biloba leaves provided a theoretical basis for the study of host adaptability mechanism and comprehensive prevention and control of Holotrichia scrobiculata Brenske. The intestinal culturable bacteria of female and male adults of Holotrichia scrobiculata Brenske feeding on Ginkgo biloba leaves were isolated, cultured and identified by LB, NA and TYA medium, combined with 16S rDNA sequence analysis, to explore the species diversity of intestinal culturable bacteria of Holotrichia scrobiculata Brenske feeding on Ginkgo biloba leaves. The composition and structure of culturable bacteria in the intestinal tract of female and male adults were different. There were 4 phyla, 11 families, 13 genera and 14 species of culturable bacteria in the intestinal tract of female adults. The dominant phylum, family, genus and species of bacteria were Firmicutes, Bacillaceae, Bacillus and Bacillus subtilis, with abundances of 86.58%, 86.56%, 62.59% and 61.64%, respectively. There were 3 phyla, 6 families, 7 genera and 11 species of culturable bacteria in the intestinal tract of male adults. The dominant phyla, families, genera and species were Firmicutes, Bacillaceae, Pediococcus and Pediococcuspentosaceus, with abundances of 98.64%, 37.74%, 34.68% and 34.68%, respectively. From the perspective of dominant bacteria, the dominant bacteria in the female intestinal tract were Bacillus subtilis, with an abundance of 61.64%, while the dominant bacteria in the male intestinal tract were Bacillus altitudinis and Pediococcuspentosaceus, with relative abundances of 31.91% and 34.68%, respectively. Pediococcuspentosaceus and Sphingomonas humi were the two common culturable bacteria in the intestines of female and male adults. The diversity of culturable bacterial species in the intestinal tract of Holotrichia scrobiculata Brenske adults feeding on Ginkgo biloba leaves was rich. The composition of intestinal bacterial species varied with the gender of female and male, and the dominant species were also different.

Key words: Holotrichia scrobiculata Brenske, Ginkgo biloba, intestinal bacteria, culturable bacteria, 16S rDNA sequence, species diversity